Wyniki wyszukiwania - Biblioteka Nauki

1

Metoda immunofluorescencji skuteczna technika w diagnozowaniu u bydla zakazenia wirusem bialaczki bydla

100%

Kaczmarczyk E. , Bojarojc-Nosowicz B.

Przegląd Hodowlany

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2009

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tom 77

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nr 03

5-6

2

Comparative analysis of the PCR and IMF methods in the diagnosis of Bovine Leukemia Virus infections

100%

Bojarojc-Nosowicz B. , Kaczmarczyk E.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 12

EN

The application of sensitive methods, enabling the detection of early infections and to diagnose the bovine leukemia virus (BLV), which ensures the eradication of this virus from herds. The aim of this study was to compare the PCR test with the IMF method for BLV diagnosis. The study involved 59 Holstein-Friesian cows aged 3-7 years and 53 heifers aged 2-6 months. Statistically significant differences were observed with respect to the effectiveness of the PCR and IMF methods during the first month after calving in cows and in older group heifers. The results of this comparison indicate that the IMF method permits the identification of a greater number of BLV carries than the PCR test. In addition, IMF may be more useful for diagnosing BLV infections than PCR.

3

Polymorphism of blood leucocyte acid phosphatase and the profile of blood plasma proteins in cows naturally infected with bovine leukaemia virus

100%

Kaczmarczyk E. , Bojarojc-Nosowicz B.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 1

4

Populations of peripheral blood lymphocytes and the activity of acid phosphatase of blood leucocytes in cows susceptible and resistant to natural infections with Bovine Leukaemia Virus

80%

Kaczmarczyk E. , Bojarojc-Nosowicz B. , Fiedorowicz A. , Majewski M.

Polish Journal of Veterinary Sciences

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2001

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tom 04

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nr 3

5

Tumor necrosis factor - alpha (TNFalpha) gene polymorphism and expression of membrane - bound TNFalpha protein on CD11bplus and IgMplus cells in cows naturally infected with bovine leukemia virus

80%

Bojarojc-Nosowicz B. , Kaczmarczyk E. , Stachura A. , Kubinska M.

Polish Journal of Veterinary Sciences

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2015

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tom 18

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nr 3

EN

The aim of this study was to determine whether SNP at position -824 (promoter region) of the TNFα gene significantly differentiates the size of IgM+, CD5+ and CD11b+ cell subpopulations and affects the expression of membrane-bound TNFα protein (mTNFα) on these cells and their susceptibility to BLV infections. In this study, significant differences were determined for the first time between TNFα genotypes and the percentage of cells with the CD11b+TNFα+p24+ immunophenotype. Furthermore, greater expansion of lymphocytes with the IgM+TNFα+p24+ immunophenotype was reported in cows with the G/G genotype than in A/A homozygotes. Cells with the above immunophenotype were more frequently observed in cows with persistent leukocytosis than in aleukemic cattle. Our results suggest that polymorphism of the TNFα -824 A>G gene and mTNFα protein expression play an important role in the pathogenesis of enzootic bovine leukosis.

6

Polymorphism in the region of bovine prion protein gene and selected haematological indices in cows naturally infected with bovine leukaemia virus

80%

Kaczmarczyk E. , Bojarojc-Nosowicz B. , Czarnik U.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 1

EN

The objective of this study was to determine whether insertion-deletion (indel) 23 bp polymorphism of the bovine prion protein (PRNP) gene differentiates the total number of leukocytes and lymphocytes and the number of virus-infected lymphocytes. The experimental materials comprised 119 Black-and-White Polish Holstein-Friesian cows. Bovine leukosis was diagnosed by an indirect immunofluorescence based on the detection of viral protein p24 in bovine lymphocytes infected with leukaemia virus (BLV). Indel23 polymorphism was determined by PCR. Blood haematological parameters (total leukocyte counts, total lymphocyte counts, and their percentages) were determined at a specialist haematological laboratory. The examined indices were analysed in three replications, at one-month intervals. It was found that indel23 pohmorphism significantly differentiated blood leukocyte counts and the total number and percentage of lymphocytes. Cows with the 23 bp del/del genotype showed significantly higher leukocyte and lymphocyte counts than animals with the remaining two genotypes. Higher values of the analysed haematological parameters noted in homozygotes with 23 bp deletion are similar to the values reported in cows affected by persistent lymphocytosis, thus pointing to an adverse effect of this genotype on the haemopoiesis process. The variations between indel23 genotypes and the number and percentage of BLV-infected lymphocytes are less obvious and more difficult to interpret.

7

Polymorphism and expression of the tumor necrosis factor receptor II gene in cows infected with the bovine leukemia virus

80%

Stachura A. , Brym P. , Bojarojc-Nosowicz B. , Kaczmarczyk E.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 1

EN

A single T>C nucleotide polymorphism (rs42686850) of bovine tumor necrosis factor receptor type II gene (TNF-RII) is located within a sequence with allele-specific affinity to bind E2F transcription factors, considered pivotal in the regulation of cell cycle and cell proliferation. The objective of the study was to determine the effect of this SNP and BLV infection on the TNF-RII gene expression at the mRNA and protein levels in peripheral blood mononuclear cells (PBMC). We noted that analyzed TNF-RII gene polymorphism influenced the expression of the TNF-RII gene at the mRNA level but only in BLV-positive cows. Concurrently, no statistically significant association was found between gene polymorphism and TNF-RII expression at the protein level. However, we found a significant effect of BLV infection status on the amount of TNF-RII mRNA and the percentage of PBMC expressing TNF-RII. These results show an unclear effect of considered T>C polymorphism on TNF-RII gene expression in bovine leukocytes and they suggest the involvement of BLV in modifying the TNF-RII expression in BLV-infected cows potentially implying the EBL (Enzootic Bovine Leukosis) associated pathogenesis.

8

Porownanie testu ELISA i PCR w diagnozowaniu zakazen wirusem enzootycznej bialaczki bydla

80%

Kaczmarczyk E. , Pawelec M. , Bojarojc-Nosowicz B.

Medycyna Weterynaryjna

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2004

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tom 60

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nr 08

824-825

9

Polymorphism of alpha - lactalbumin gene and changes of the profile of peripheral blood lymphocytes in cows naturally - infected with bovine leukaemia virus

80%

Kaczmarczyk E. , Bongarc E. , Bojarojc-Nosowicz B. , Malolepszy J.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 4

10

Polymorphism in the promoter region of the tumor necrosis factor-alpha gene in cattle herds naturally infected and uninfected with the Bovine Leukemia Virus

80%

Bojarojc-Nosowicz B. , Kaczmarczyk E. , Stachura A. , Kotkiewicz M.

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tom 14

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nr 4

EN

The objective of this study was to describe and compare the genetic structure (TNF-α-position 824) of dairy cattle herds infected and not infected with the bovine leukemia virus (BLV). The results of the present study indicate that BLV-positive herds were characterized by similar genetic structure (TNF-α-824A/G). The genetic equilibrium in these herds was preserved, but a tendency to increased frequency of G/G homozygotes was found. The genetic structure of the healthy herd differed considerably from that of leukemic herds.

11

Prion protein gene (PRNP) polymorphism and indicators of secretion disorders of the mammary gland in cows naturally infected with bovine leukaemia virus (BLV)

80%

Czarnik U. , Strychalski J. , Bojarojc-Nosowicz B. , Kaczmarczyk E.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 4

12

Polymorphisms in the bovine tumour necrosis factor receptor type two gene (TNF-RII) and cell subpopulations naturally infected with bovine leukaemia virus

80%

Stachura A. , Bojarojc-Nosowicz B. , Kaczmarczyk D. , Kaczmarczyk E.

Journal of Veterinary Research

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2019

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tom 63

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nr 2

13

Prion protein gene polymorphism and blood lymphocyte profile in cows naturally infected with bovine leukemia virus

80%

Kaczmarczyk E. , Bojarojc-Nosowicz B. , Czarnik U. , Strychalski J.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 3

14

Composition of protein fractions of blood plasma in heifer calves naturally infected with bovine leukaemia virus

80%

Kaczmarczyk E. , Jaglowska B. , Bojarojc-Nosowicz B.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 4

15

Polymorphism of the alpha-lactalbumin gene and populations and subpopulations of peripheral blood lymphocytes in heifers naturally infected with bovine leukaemia virus

80%

Kaczmarczyk E. , Malolepszy J. , Bojarojc-Nosowicz B. , Bongarc E.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 3

16

Natural BLV infection and polymorphism within the 5'flanking region of the alpha-lactalbumin gene in Black-and-White breed cattle

80%

Bojarojc-Nosowicz B. , Kaczmarczyk E. , Bongarc E. , Malolepszy J.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 4

17

Polymorphism of blood leucocyte acid phosphatase and haematological indices during the first months after calving in cows naturally-infected with the bovine leukaemia virus

80%

Kaczmarczyk E. , Bojarojc-Nosowicz B. , Fiedorowicz A.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 1

18

Profile of peripheral blood lymphocytes in the first months of life of heifers originating from a leukaemic herd

80%

Kaczmarczyk E. , Fiedorowicz A. , Bojarojc-Nosowicz B. , Majewski M.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 4

19

Leucocyte acid phosphatase and metabolic efficiency of phagocytes in the first lactation trimester of cows from a leukaemic herd

80%

Kaczmarczyk E. , Bojarojc-Nosowicz B. , Fiedorowicz A.

Journal of Applied Genetics

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2005

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tom 46

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nr 1

EN

In Black-and-White cattle, polymorphism of acid phosphatase (AcP) of blood leukocytes is determined by a pair of autosomal alleles. The aim of the study was to determine the relationship between AcP polymorphism and the metabolic efficiency of phagocytes in the first months after calving of cows naturally infected with the bovine leukaemia virus. The studied population consisted of 91 Black-and-White cows aged 3-6 years, from one herd. Enzootic bovine leukaemia (EBL) was diagnosed with the immuno-enzymatic ELISA method and a PCR molecular test. Additionally, agarose gel electrophoresis and the cytochemical method were used to determine the AcP polymorphism and activity in leukocytes. The metabolic activity of phagocytes was determined by the nitroblue tetrazolium (NBT) reduction test. Significant differences in metabolic efficiency of granulocytes were observed between cows representing different AcP phenotypes. No significant differences in levels of the analysed indices were observed between the EBL-positive and EBL-negative cows and between the three subsequent months after calving.

20

Relationship between polymorphism of the alpha-lactalbumin gene and susceptibility of lymphocytes to infections with bovine leukaemia virus and to apoptosis in naturally infected cows

80%

Bojarojc-Nosowicz B. , Kaczmarczyk E. , Duzowski M.

Bulletin of the Veterinary Institute in Puławy

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2008

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tom 52

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nr 2

EN

A study was undertaken to analyse the effect of polymorphism of α-lactalbumin (ALA) gene at position -1689 on the susceptibility of lymphocytes to the infection with bovine leukaemia virus (BLV) and to undergoing apoptosis during the first three months after calving. The experiments covered 97 Black-and-White breed cows, aged 3-8 years, from three herds. The infection with BLV was diagnosed on the basis of the presence of the p24 protein of the virus identified by means of the fluorescent antibody test in peripheral blood lymphocytes. The BLV-positive and apoptotic cells were registered with the use of a fluorescence microscope. Analyses of white blood cell counts enabled separating the BLV- infected cows into groups with the aleukaemic form and with persistent lymphocytosis. The results obtained demonstrated a relationship between polymorphism of ALA gene and the susceptibility of lymphocytes to BLV infections and to undergoing apoptosis. The current study, together with the authors' previous research, suggests the possibility of ALA gene participation in mechanisms of BLV infection pathogenesis.