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tom 41
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EN
Experimental cucumber and tomato plants were cultivated under glasshouse conditions with or without PGPR. Young plants were infested with spider mites (T. urticae - cucumber and T. cinnabarinus - tomato). Leaves were analysed for protein and amino acid concentration. In infested leaves of the cucumber cultivar, susceptible to spider mites (Corona), an important decrease in protein content occurred in both bacterized and nonbacterized plants. Amino acid content was higher in mile infested leaves and the presence of PGPR did not influence this phenomenon. The content of proteins was also studied in the leaves of the less susceptible cucumber cultivar (Aramis). Spider mile feeding caused a small decrease in their concentration. The presence of bacteria in the root system caused an increase of soluble proteins in the leaves. In infested leaves of the highly susceptible tomato cultivar (Romatos), cultivated without bacteria, an evident increase of amino acid content was found opposite to plants with PGPR. The protein concentration was also increased in injured leaves of this cultivar but the presence of bacteria in the root system of mite infested plants caused a decrease of these compounds in their leaves. lnjured foliage of the less susceptible tomato cultivar (Sionka) had a lower protein concentration in bacterised plants as compared to those nonbacterised and both controls.
PL
Rośliny ogórka i pomidora uprawiano w warunkach szklarniowych, w obecności bakterii sfery korzeniowej (PGPR) i bez bakterii. Młode rośliny porażano przędziorkami (Tetranychus urticae Koch - ogórek i Tetranychus cinnabarinus Boisduval - pomidor). Liście porażonych roślin poddano analizie na zawartość białek rozpuszczalnych i aminokwasów. W porażonych liściach wrażliwej na przędziorki odmiany ogórka (Corona) zaobserwowano znaczny spadek zawartości białek zarówno w przypadku roślin uprawianych bez bakterii jak i w obecności PGPR. W porażonych liściach tej odmiany wzrastała natomiast zawartość wolnych aminokwasów. Badano również zawartość białek rozpuszczalnych w liściach mniej podatnej na przędziorki odmiany ogórka (Aramis). Żerowanie przędziorków powodowało kilkuprocentowe obniżenie zawartości białek, a obecność PGPR w sferze korzeniowej porażonych roślin prawie nie miała wpływu na ich poziom w liściach. W porażonych liściach wrażliwej na przędziorki odmiany pomidora (Romatos) stwierdzono, podobnie jak w przypadku ogórka, istotny wzrost zawartości wolnych aminokwasów. U porażonych roślin tej odmiany, uprawianych w obecności PGPR, obserwowano natomiast spadek zawartości aminokwasów w porównaniu z roślinami kontrolnymi nie traktowanymi bakteriami. W porażonych liściach tej odmiany zaobserwowano wzrost zawartości białek rozpuszczalnych zarówno u roślin z PGPR jak i bez bakterii. Nie obserwowano tego zjawiska u mniej podatnej na przędziorki odmiany pomidora Slonka.
EN
Our investigations suggest that the usually observed avoidance by O. ununguis of current year’s twigs of P. glauca ‘Conica’ as a place for feeding and development may be connected with their morphological and anatomical structure as well their biochemical composition. Needles of the current year’s twigs were covered with a thicker layer of wax and formed more extensive epidermis cell walls, in comparison with needles of 1-year-old twigs. Young needles also showed lower concentration of reducing sugars and soluble proteins, which might additionally reduce their nutritional suitability for mite development. Differences were also found between the 1- year-old growth and current growth in contribution of essential oils and a range of other compounds. Further analyses of the effect of these compounds on mite behavior may provide additional explanation of the feeding preferences of the mite.
EN
Transgenic maize with cry1Ab gene is resistant to target insect – Ostrinia nubilalis (Hbn.). Among non-target herbivorous insects, the rose-grain aphid (Metopolophium dirhodum Walk.) is one of known cereal aphid species. The aim of this study was to assess the behaviour and bionomy of M. dirhodum on three maize cultivars: transgenic (YG), isogenic (ISO) and conventional Bosman in greenhouse and laboratory conditions. To assess aphids preference to maize cultivars the “free choice test” was performed. The number of aphids that settled on preferred plants (at the 4-leaf stage) of various maize cultivars was counted after 24 and 48 h. For the next 8 days new-born nymphs and apterous aphids were counted. Bionomy parameters of females were evaluated on leaves cut from various cultivars and closed in Münger cages. This study was performed in growth chambers using single aphid as one replication. In greenhouse bioassay screens (“free choice test”) the negative impact of transgenic maize on aphids wasn’t stated. However on YG plants the rate of M. dirhodum population development was slower. Similarly, in laboratory tests, aphids that fed on YG plants had lower fecundity, shorter longevity and reproductive period than those fed on ISO and Bosman cvs. This suggests that among cultivars assessed, for some reason the maize plants of YG cv. are the most antibiotic to M. dirhodum.
EN
Maize (Zea mays L.) hybrid with the Bt transgene from the bacterium Bacillus thuringensis (Bt) (DKC 3421 YG) and its non-Bt near-isoline (DKC 3420) as well as Polish cultivar Bosman (HR Smolice) were subjected to drought stress and/or the two-spotted spider mite (Tetranychus urticae, Acarina: Tetranychidae) feeding to assess changes in the relative water content (RWC) and dry weight (DW) in leaves of different age (leaf: 7th, 8th, 9th, 10th). All plants were at the vegetative stage and grew under greenhouse conditions. The effect of both abiotic and biotic stress was analysed 4 and 6 days after treatment. The experiments conducted in two series showed that among examined maize cultivars, T. urticae feeding decreased RWC in Bosman cv. leaves only, while drought altered leaf RWC of plants in a similar manner – the older the leaf, the greater reduction of RWC. Drought alone and drought accompanied by T. urticae feeding significantly decreased RWC of maize leaves in all cultivars (DKC 3421 YG, DKC 3420, Bosman). Concluding, drought had a greater impact on leaf RWC of young maize plants than mite feeding.
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