Wyniki wyszukiwania - Biblioteka Nauki

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Natural xenobiotics inducing apoptosis in hepatoma cells: in vitro study

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Starosta M. , Tyszka-Czochara M. , Bukowska-Strakova K. , Dołhańczuk-Śródka A.

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tom Vol. 12, No. 2

335--343

EN

The term “xenobiotics” refers to substances which are not produced within the human body, but may occur in human environment. Some of biologically active chemicals may be synthesized by plants. Many studies have been conducted with regard to plant secondary metabolites and the beneficial impact of various plant-derived compounds on human health has been demonstrated. Recent research have shown chemopreventive properties of catechins. (+)-catechin ((2R,3S)-2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5,7-triol) and (-)-epigallocatechin gallate (EGCG; [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)chroman-3-yl] 3,4,5-trihydroxybenzoate) are compounds abundant in daily human nutrition. Growing body of evidence reports pro-apoptotic effects of catechins. Both catechins have been shown to modulate intracellular pathways network in tumor cells thereby regulating signaling cascades, which results in programmed death of cancer cells, apoptosis. Apoptosis is one of the most potent mechanisms to defense against cancer. During apoptosis, the activation of caspases results in biochemical and morphological changes associated with specific changes in cell phenotype. Besides apoptosis, tumor cells may be also eliminated from organism via necrosis, however the latter process may evoke local inflammation, which in turn may result in detrimental effects within the body. Xenobiotics with potential to cause apoptosis of cancer cells may be considered in modern combined anticancer therapies and/or to assist existing treatments.

PL

W środowisku występują substancje obce o charakterze ksenobiotyków, czyli związków chemicznych niesyntetyzowanych w sposób naturalny w ciele człowieka. Wiele takich aktywnych biologicznie związków może być produkowanych przez rośliny. Przeprowadzone dotychczas badania wskazują, że drugorzędowe metabolity roślinne mogą wywierać pozytywny wpływ na zdrowie człowieka. Na przykład wyniki ostatnich obserwacji potwierdziły chemoprewencyjne właściwości katechin. (+)-katechina (ang. (2R,3S)-2-(3,4dihydroxyphenyl)-3,4dihydro-2H-chromene-3,5,7-triol) oraz (-)-galusan epigallokatechiny (ang. EGCG; [(2R,3R)-5,7-dihydroxy-2(3,4,5-trihydroxyphenyl)chroman-3-yl] 3,4,5-trihydroxybenzoate) to związki o charakterze ksenobiotyków występujące w diecie człowieka. Ostatnie odkrycia zwracają uwagę w szczególności na proapoptotyczne właściwości związków z tej grupy. Obydwa wymienione związki chemiczne mogą modyfikować przebieg wewnątrzkomórkowych ścieżek sygnałowych w komórkach nowotworowych. Takie oddziaływanie może uruchamiać kaskadę aktywności prowadzącą do apoptozy, programowanej śmierci komórki neoplastycznej. Apoptoza jest jednym z mechanizmów, które wykorzystuje się w nowoczesnych terapiach przeciwnowotworowych. Apoptozę charakteryzuje szereg zmian w przebiegu procesów biochemicznych i morfologii komórki. Komórki nowotworowe mogą być eliminowane również na drodze nekrozy, jednakże ten typ śmierci komórkowej często prowadzi do lokalnego stanu zapalnego, co jest zjawiskiem niekorzystnym dla organizmu. Drugorzędowe metabolity roślinne, takie jak (+)-katechina i (-)-galusan epigallokatechiny, mogą wykazywać zdolność do wywoływania śmierci apoptotycznej komórek nowotworowych i potencjał ten może być wykorzystywany w nowoczesnych przeciwnowotworowych terapiach kombinowanych lub do wspierania istniejącego leczenia.

2

Recent advances in the role of AMP-activated protein kinase in metabolic reprogramming of metastatic cancer cells: targeting cellular bioenergetics and biosynthetic pathways for anti-tumor treatment

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Tyszka-Czochara M. , Konieczny P. , Majka M.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 3

3

Effect of fibrates on branched-chain alpha-ketoacid dehydrogenase complex activity

100%

Knapik-Czajka M. , Tyszka-Czochara M. , Jaskiewicz J.

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nr 2

4

Identification of lipid derivatives in Hep G2 cells

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Gdula-Argasinska J. , Gabracik A. , Tyszka-Czochara M. , Wozniakiewicz M. , Pasko P. , Czepiel J.

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2013

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tom 60

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nr 4

EN

Metabolism of polyunsaturated fatty acids results in biosynthesis of mediators with different physiological effects. These metabolites include prostaglandins, prostacyclins, isoprostanes and others that are important signalling molecules and regulate a variety of physiological and pathophysiological processes including inflammation. Prostaglandins and isoprostanes are produced by either non-enzymatic lipid peroxidation or by enzyme-induced peroxidation (cyclooxygenases and lipoxygenases). They are used as biomarkers of oxidative stress. The aim of our study was to assess the effect of eicosapentaenoic acid (EPA) supplementation with added benzo(a)pyrene (BaP) on HepG2 cells by using a UHPLC/MS-TOF method. This rapid and simple method was developed for the identification, separation and quantification of 8-iPGF3α, PGF3α, 8-isoPGF2α and 5-iPF2α in cultured cells. The UHPLC/MS-TOF method was validated. The calculated limit of detection was in the range of 0.16-0.50 ng/mL, precision (% RSD): 1.2-2.1% and recoveries better than 88%. This method empowered qualitative and quantitative analysis of the selected individual prostaglandins derived from arachidonic acid and eicosapentaenoic acid from cell extracts.

5

Porównanie aktywności antyoksydacyjnej i całkowitej zawartości polifenoli w kiełkach i różyczkach brokułów

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Pasko P. , Prochownik E. , Galanty A. , Barton H. , Tyszka-Czochara M. , Zagrodzki P. , Zachwieja Z.

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nr 2

6

Antihistaminic activity of lidocaine derivatives in the isolated guinea pig ileum

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Librowski T. , Pytka K. , Salat K. , Rapacz A. , Moniczewski A. , Gdula-Argasinska J. , Tyszka-Czochara M.

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tom 54

EN

Local anesthetics (LAs) are drugs that inhibit membrane depolarization by reducing sodium conductance. In addition to the most prominent and well-defined function of LAs described above, numerous studies have shown that LAs possess many other properties, such as anti-inflammatory, anti-arrhythmic or analgesic activities. Furthermore, LAs are structurally similar to histamine H-receptor antagonists. Thus, LAs can be considered antihistaminic drugs. The aim of the present study was to determine and compare the antihistaminic effects of lidocaine derivatives - JCB-1, JCB-2 and JCB-3 - on the isolated guinea pig ileum. Only JCB-1 and JCB-2 at the highest concentration (10 4M) showed the antihistaminic effect. The studied compounds showed a dose-dependent effect. Interestingly, lower concentrations of the studied compounds (10 8M and 10"I0M) caused the opposite effect - increased the contraction of the ileum induced by histamine. These effects necessitate further studies. Nevertheless, this is the first time that JCB compounds have been demonstrated to possess antihistaminic properties.

7

Differential effects of zinc supplementation on the anti-inflammatory activity of ketoprofen in rats

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Lipkowska A. , Gawel M. , Olbert M. , Gdula-Argasinska J. , Tyszka-Czochara M. , Rij E. , Rak A. , Razny K. , Salat K. , Librowski T.

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2013-2014

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tom 55-56

8

Cytotoxic effects of alpha-lipoic acid and its biodegradation derivatives - a comparative study in an in vitro model of murine embryo fibroblasts

63%

Tyszka-Czochara M. , Dudek M. , Rak A. , Salat K. , Gluch-Lutwin M. , Bilska-Wilkosz A. , Kwiecien I. , Pasko P. , Gdula-Argasinska J. , Librowski T.

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2013-2014

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tom 55-56

9

Influence of gamma-butyrolactone derivatives with analgesic properties on the prostaglandin E2 level and gastric mucosa in rodents

51%

Salat K. , Librowski T. , Gdula-Argasinska J. , Tyszka-Czochara M. , Jastrzebska-Wiesek M. , Moniczewski A. , Rapacz A. , Pytka K. , Filipek B. , Wieckowski K. , Malawska B.

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tom 54

EN

Our recent studies indicate that some derivatives of gamma-butyrolactone (GBL) possess analgesic properties in rodent models of pain and inflammation. Despite this pronounced biological activity, the mechanism of action of these GBL still remains unclear. Searching for the plausible mechanism of their action we investigated whether they were (similarly to nonsteroidal anti-inflammatoiy drugs) capable of influencing the PGE2 level in mice subjected experimentally to zymosan-induced peritonitis. The PGE2 level was evaluated in vitro by means of the ELISA assay. We also investigated the influence of orally administered GBL on the rat gastric mucosa in post mortem studies. We have demonstrated that neither of the GBL derivatives influences the PGE2 level in zymosan-induced inflammation of the peritoneal cavity in mice. The investigated compounds are also devoid of adverse effects within the stomach, typical of cyclooxygenase inhibitors, which also indirectly suggests that their analgesic and anti-inflammatory activities result from mechanisms other than cyclooxygenase inhibition.