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Comparative hybridization of the Y chromosome in selected species of Bovidae

100%

Kozubska-Sobocinska A. , Slota E. , Pienkowska-Schelling A. , Schelling C.

nr 1

Probe generated by Y chromosome microdissection in useful for analysing the sex chromosomes of the domestic horse

88%

Pienkowska-Schelling A. , Bugno M. , Owczarek-Lipska M. , Schelling C. , Slota E.

nr 2

173-178

Microdissection of chromosomes is an invaluable tool for physical isolation of single chromosomes, chromosome arms or chromosome bands. In the present study we have generated a fluorescence in situ hybridization (FISH) probe from the microdissected Y chromosome of the domestic horse. For the identification of the Y chromosome, we used the CBG technique. A biotinlabelled probe in the FISH technique gave a specific signal on all Y chromosomes and an additional signal in the interstitial part of the long arm of the X chromosome. This area showed an additional C band detected by the CBG method and characteristic for this chromosome. The probe obtained can therefore be used for analysis of the Y and X chromosomes and their copies in the genome, and, in the case of the X chromosome, also for gene mapping.