Wyniki wyszukiwania - Biblioteka Nauki

1

Guarantee of constant train approach warning time at level crossing system

100%

Kmet V. , Rastocny K.

Archives of Transport System Telematics

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2009

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tom Vol. 2, iss. 1

7-11

EN

Th is paper deals with an optimisation problem of train approach warning time at an automatic level crossing system. It discusses possibilities for reducing the train approach warning time and benefi ts resulting from speed discriminators application.

2

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

80%

Kmet V. , Drugdova Z. , Kmetova M. , Stanko M.

Annals of Agricultural and Environmental Medicine

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2013

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tom 20

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nr 2

EN

With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

3

The effect of pectynolitic yeasts on rumen microflora

80%

Kmet V. , Jonecova Z. , Stachova M.

Journal of Animal and Feed Sciences

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1992

|

tom 01

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nr 2

165-170

4

A note on the influence of microgravity on the microbial endoecosystem of Japanese quail

80%

Laukova A. , Boda K. , Kmet V.

Journal of Animal and Feed Sciences

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1993

|

tom 01

|

nr 3-4

295-301

5

Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse

70%

Gregova G. , Kmetova M. , Kmet V. , Venglovsky J. , Feher A.

Annals of Agricultural and Environmental Medicine

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2012

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tom 19

|

nr 1

EN

The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.

6

Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater

70%

Cornejova T. , Venglovsky J. , Gregova G. , Kmetova M. , Kmet V.

Annals of Agricultural and Environmental Medicine

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2015

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tom 22

|

nr 3

7

New insight into genotypic and phenotypic relatedness of Staphylococcus aureus strains from human infections or animal reservoirs

51%

Lisowska-Lysiak K. , Kosecka-Strojek M. , Bialecka J. , Kasprowicz A. , Garbacz K. , Piechowicz L. , Kmet V. , Savini V. , Miedzobrodzki J.

Polish Journal of Microbiology

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2019

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tom 68

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nr 1

EN

Staphylococcus aureus is a common human and livestock opportunistic pathogen, and there is evidence of animal to human transmission.This paper aimed to recognize properties of the isolates from collections of human and livestock S. aureus strains and to estimate compat-ibility of results based on phenotypic tests, microarrays and the spa typing methods. The second goal was to study differences between human and animal isolates in terms of specificity of their hosts and the strain transmission among various hosts. Most strains showed multi-susceptible profiles and produced enzymes on a high level, and they were phenotypically and genetically similar. However, in contrast to the Polish bovine mastitis strains, the Slovakian strains were multi-resistant. In this research, the strains showed significant differences in terms of their phenotypic manifestations and the presence of hemolysins genes; however, other enzyme-encoding genes correlated to a higher extent with the microarrays results. Interestingly, there was a lack of enterotoxin genes in human Poultry-like protein A+ strains in comparison to other human strains. Our study showed that differences between virulence profiles of the human and animal strains cor-related with their origin rather than their hosts, and any trait allowed clearly distinguishing between them based on the microarray results.