Wyniki wyszukiwania - Biblioteka Nauki

1

Zastosowanie technologii macierzy ekspresyjnych, proteomicznych oraz tkankowych w badaniach nad onkogenezą u ssaków

100%

Kempisty B. , Zawierucha P. , Piotrowska H. , Nowicki M.

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tom 67

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nr 11

EN

Technological progress in the past few years has made it possible to apply techniques based on microarrays in the analyses of cancerogenesis. These techniques can be divided into several groups according to the scale of research: (1) gene expression analysis, (2) protein analysis, (3) tissue research. The gene expression analysis makes it possible to compare the levels of gene activity in cancer tissue with those in reference tissue, and to evaluate the progression of cancer and its reaction to treatment. The analysis of proteins provides information on their functional and structural characteristics, and throws light on the protein-ligand interaction and the relations between the presence or absence of certain proteins in specific physiological states. Tissue microarrays are applied to analyze cancer markers and to identify DNA, RNA and proteins. This article presents selected aspects of microarray research and discusses the molecular aspects of cancerogenesis in dogs, referring to several types of genes associated with cancers.

2

Conductive atomic force microscope for investigation of thin-film gate insulators

88%

Wielgoszewski G. , Gotszalk T. , Woszczyna M. , Zawierucha P. , Zschech E.

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3

Uniwersalny sterownik na bazie mikrokontrolera ATmega128 dla mikroskopii bliskich oddziaływań

88%

Garczyński K. , Zielony M. , Zawierucha P. , Kopiec D. , Gotszalk T.

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2010

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tom Vol. 51, nr 9

69-72

PL

Artykuł przedstawia konstrukcję i zastosowanie tanich i uniwersalnych sterowników dla systemów pomiarowych w mikroskopii bliskich oddziaływań. Głównym założeniem projektu jest minimalizacja kosztów budowy takiego systemu poprzez zastąpienie głównej jednostki sterującej, zbudowanej na procesorze sygnałowym, mikrokontrolerem jednoukładowym oraz logiką programowalną. Przedstawione zostaną algorytmy komunikacji między magistralą sterującą i komponentami systemu (karty ADC, DAC) oraz wyniki pomiarów.

EN

This paper shows a construction of universal and Iow cost control drivers for measure systems using in atomic force and scanning microscopy. Main point of this project is to minimize cost of this system and replace main central processing unit, expensive signal processor, by 8bits microcontroller and programmable logic device. l'd like to show algorithms describing Communications with PC, system components like Analog-Digital and Digital-Analog cards and present some measure effects.

4

Molekularne mechanizmy procesów angiogennych i ich udział w patogenezie miażdżycy

75%

Zawierucha P. , Kempisty B. , Sosinska P. , Wojtowicz K. , Nowicki M. , Witkiewicz W.

Postępy Biologii Komórki

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2012

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tom 39

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nr 4

5

Skalowanie optycznego czujnika sił tarcia mikroskopu sił atomowych

75%

Masalska A. , Zawierucha P. , Woszczyna M. , Gotszalk T. , Ritz Y. , Zschech E.

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2008

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tom Vol. 49, nr 6

69-71

PL

Badania zjawisk tarcia, ścierania, poślizgu czy zużycia trybologiczne-go w nanoskali mogą być z powodzeniem przeprowadzane za pomocą mikroskopu sit atomowych (ang. Atomie Force Microscope-AFM) z optyczną detekcją ugięcia belki pomiarowej, która pełni rolę optycznego czujnika sit tarcia. Uzyskanie informacji ilościowych wymaga jednak uprzedniego zastosowania procedury kalibracyjnej pozwalającej na konwersje danych pomiarowych na dane o znaczeniu fizycznym. W niniejszej pracy zaprezentujemy wyniki skalowania mikroskopu sił atomowych metodą odwzorowania pochylenia wzorcowego (ang. Wedge Calibration Method [1]) z wykorzystaniem struktury firmy MikroMasch (TGF11) i struktury wykonanej za pomocą skupionej wiązki jonów (ang. Focused Ion Beam-FIB).

EN

Mechanisms of friction, adhesion, lubrication and wear in nanoscale : can be successfully investigated by means of atomic force microscope (AFM) using optical beam deflection sensing. However, to obtain quantitative information the calibration procedure for conversion of measured data to friction forces has to be applied. In order to calibrate our atomic force microscope we applied direct procedure called wedge calibration method [1]. The results of friction force calibration using commercially available grating (TGF11) and dedicated specimen fabricated by focused ion beam (FIB) milling are presented in this paper. We show experimental results for nanotribology on silicon of two different crystallographic orientation, mica and HOPG as well.

6

The in vitro culture supplements and selected aspects of canine oocytes maturation

75%

Zawierucha P. , Bukowska D. , Kempisty B. , Piotrowska H. , Brussow K. , Jaśkowski J. , Nowicki M.

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nr 1

7

Uniwersalny mikroprocesorowy miernik impedancji elektrycznej

75%

Rudek M. , Woźniak K. , Wojciechowski T. , Woszczyna M. , Zawierucha P. , Gotszalak T.

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tom R. 85, nr 11

175-177

PL

W artykule przedstawiona została zasada działania oraz konstrukcja mikroprocesorowego miernika impedancji charakteryzującego się szerokim zakresem mierzonej impedancji od 1 k[om] do 10 M[om] oraz stosunkowo szerokim pasmem pomiarowym od 1 kHz do 100 kHz. Zwarta budowa, niewielkie rozmiary oraz niska cena prezentowanego urządzenia sprawiają, że w mniej precyzyjnych pomiarach stanowi alternatywę dla drogich, komercyjnych rozwiązań tego typu.

EN

In this article theory of operation and design of a microprocessor electrical impedance measurement device is presented. It features broad input impedance range from 1 k[om] to 10 M[om] and relatively wide measurement bandwidth from 1 kHz to 100 kHz. Compact design, small dimensions and low price are its advantages and make an alternative choice to expensive commercial equipment.

8

The in vitro culture supplements and selected aspects of canine oocytes maturation

75%

Bukowska D. , Kempisty B. , Piotrowska H. , Zawierucha P. , Brussow K. P. , Jaskowski J. M. , Nowicki M.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 1

EN

The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved. In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.

9

Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych

51%

Kempisty B. , Walczak R. , Sniadek P. , Dziuban J. , Piotrowska H. , Zawierucha P. , Zaorska K. , Antosik P. , Bukowska D. , Jaskowski J. M. , Nowicki M. , Zabel M.

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tom 67

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nr 10

EN

Evaluation of oocyte developmental competence has an important influence on the ability of these cells to attain maturation, successful fertilization, development of embryo to the blastocyst stage and proper implantation. Factors determining the reproductive potential of gametes included: (1) expression of important transcription factors, (2) epigenetic changes, which influence the silencing of selected genes transcription, (3) transcription regulation, and (4) post-transcriptional regulation. The epigenetic changes mainly include: DNA methylation, histones modifications and changes in chromatin structure in the oocytes. In several studies, the association between oocyte morphology (mostly determined by cumulus cell layers and granularity of cytoplasm) and the ability of these cells to attain maturation and fertilization has been described. The use of biochemical, metabolomical and molecular markers is the most frequently applied tool in the assessment of oocyte developmental competence. However, most of these methods are invasive and lead to the decreased viability of the analyzed cells. Searching for new, objective and noninvasive techniques leads to the development of a microfluidic chip system, which shows the physical (spectral) properties of oocytes and embryos in comparison to the biological parameters. In this article, selected issues associated with the genetic regulation of such processes as: maturation of oocytes, fertilization and early stages of embryonic development, have been presented. Moreover, the regulation of transcription and post-transcriptional modification during oogenesis and embryogenesis in mammals, with special relation to pigs and the possibilities of applying of microfluidics in assessment of oocyte and embryo developmental competence was shown.