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Content available remote 3D matrigel culture improves differentiated functions of HepG2 cells in vitro
100%
Kinasiewicz J. , Kawiak J. , Weryński A.
Biocybernetics and Biomedical Engineering
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2006
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tom Vol. 26, no. 4
47-54
EN
In our study, the influence of culture conditions (monolayer and 3D Matrigel) of hepatocyte cell line (buman hepatoma cell line, HepG2) on their metabolic activity was assessed. Albumin synthesis by HepG2 cells was significantly higher in Matrigel culture as compared to monolayer culture of the cells. 3D culture improved urea production by HepG2 cells. The detoxification experiments revealed that HepG2 cells do not metabolize lidocaine neither in monolayer nor in 3D culture. 3D culture significantly improved cell polarity analyzed by F-actin staining and carboxyfluorescein excretion. In conclusion, the 3D Matrigel culture improves highly differentiated hepatic functions of HepG2 cells.
2
Content available remote The influence of transplantation of encapsulated porcine islets on glycemia in diabetic mice
100%
Kinasiewicz J. , Sabat M. , Godlewska E. , Orłowski T.
Biocybernetics and Biomedical Engineering
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2004
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tom Vol. 24, no. 4
67-70
EN
Transplantation of pancreatic islets is a promising therapy of type I diabetes. Human donor shortage may be alleviated by transplantation of porcine islets. Unfortunately to prevent graft rejection immunosuppresion or immunoisolation is mandatory. In this paper we demostrate, that xenotransplantation of porcine islets microencapsulated in algiate/poly-L-lysine/alginate microcapsules to streptozotocin diabetic mice can reverse hypoglykemia but unfortunatelly the fenomenon of primary graft nonfunction (PNF) can frequently be noted.
3
Content available remote Electrostatic microencapsulation of living cells
88%
Lewińska D. , Bukowski J. , Kożuchowski M. , Kinasiewicz J. , Weryński A.
Biocybernetics and Biomedical Engineering
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2008
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tom Vol. 28, no. 2
69-84
EN
Microencapsulation of different biologically active material for diverse applications have received increasing interest over the last 20 years. Microencapsulation of living cells seems to be a very promising and prospective technology, especially useful in biotechnology and medical applications. One of the most convenient and precise method for this purpose is an electrostatic technique. Electrostatic droplet generation could be performed using single- or multi-nozzle devices, significantly improving efficiency of the process. The usage of an impulse voltage generator allows to manufacture spherical and uniform microbeads with sizes from 0.2 to 3.0 mm of very narrow size distribution. Proposed two-liquid droplet electrostatic formation technique provides preparation of core/shell microbeads, where all cells are immobilized deeply inside a matrix and surrounded with cell-free polysaccharide layer. Such a solution prevents from cell protrusion out of the capsule. Applied electrostatic field is safe for encapsulated living cells and does not cause any cell dysfunction.
4
Content available The influence of porcine pancreas digestion parameters and islet histomorphology on islet isolation outcome
75%
Kinasiewicz J. , Sabat M. , Antosiak-Iwanska M. , Godlewska E. , Sitarek E. , Orlowski T.
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tom 14
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nr 2
EN
Transplantation of the pig islets of Langerhans is considered as the future treatment for patients suffering from type I diabetes mellitus. Despite the adaptation of modified Ricordi method and highly purified collagenase, the results of pancreas digestions are precarious. Selection of proper donor and optimal digestion procedure are fundamental. The aim of this study was to assess the impact of pancreas procuring parameters on pig islets yield. The pancreata were harvested from 69 market sows weighting over 150 kg. After intraductal injection of cold collagenase solution pancreata were transported in UW solution or under conditions of two layer method (TLM). In laboratory pancreata were digested at 37℃ according to Ricordi isolation method or stationary in the bottle. The particular parameters of isolation procedure were considered as substantial. Pig weight, volume of infused collagenase solution, TLM application and pancreas dividing before digestion positively affected islet yield. Additionally, the influence of pancreatic islet tissue histomorphology on isolation outcome was studied. Proper donor selection as well as adequate digestion parameters could improve pig islet recovery during islet isolation.
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Content available The influence of porcine pancreas digestion parameters and islet histomorphology on islet isolation outcome
75%
Kinasiewicz J. , Sabat M. , Antosiak-Iwańska M. , Godlewska E. , Sitarek E. , Orłowski T.
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