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1

Wykorzystanie biotechnologii w diagnostyce i zwalczaniu zakażeń wirusem białaczki bydła

100%

Kuzmak J.

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nr 1

2

Serologiczna diagnostyka białaczki bydła: efekty uzycia wydzieliny przedsiarowej do badania krów w okresie zasuszenia

63%

Kuzmak J. , Grundboeck-Jusko J.

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nr 04

RU

Цель рабозы состояла в оценке пригодности серологического исследования домолозивного секрета засушенных короо в св стадах, инфицированных вирусом еЬЬ. У 103 засушенных коров параллельное исследование сыворотки крови и домолозивного секрета критерием ГО, показало положительную реакцию у 73 голов, тогда как исследование самой сыворотки дало положительный результат,. только у 29 голов. Из проведенных наблюдений вытекает необходимость применения домолозвиного секрета в разпознавании еЬЪ.

EN

The aim of the study was an improvement of serological diagnosis of bovine leukosis in the precalving period when the level of blood serum antibodies shows a significant drop. Serum and precolostral udder secretion of 103 cows in dry period were paralelly examined in the AGID test showing presence of specific antibodies in 73 animals. However, examinations of sera gave positive results only in 29 animals. The results indicate that the precolostral udder secretion should be used in a routine serological examination of cows.

3

Spumawirusy - zagadkowe retrowirusy

63%

Materniak M. , Kuzmak J.

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nr 07

744-748

EN

Spumaviruses belong to the family of Retroviridae, genus Spumavirus. They are also commonly known as foamy viruses or syncytial viruses because of inducing the spectacular cytopathic effect in tissue culture, which produces vacuolated “foamy” forms and the appearance of typical syncytia. Foamy viruses are widespread and have been isolated from many mammals, such as primates, pets and livestock animals. The resultant infection is persistent and infected individuals develop a strong antibody response. Although these viruses cause a strong cytophatic effect in cell cultures, they have never been linked to particular symptoms or pathalogical developments. During the last few years the research on spumaviruses has increased, mainly due to their possible zoonotic potential and thus a great deal of activities connected with the development of diagnostic assays and pathogenicity studies were carried out. This paper reviews the present stage of knowledge on spumaviruses found in humans and animals.

4

Latencja retrowirusow na przykladzie wirusa enzootycznej bialaczki bydla [BLV]

63%

Kuzmak J. , Rola M.

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nr 4

335-344

5

Występowanie przeciwciał dla wirusa enzootycznej białaczki bydła w siarze krów białaczkowych

63%

Grundboeck-Jusko J. , Kuzmak J.

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nr 06

RU

Исследовали уровень антител в сыворотке крови 17 положительных серологически коров в период 8 недель перед родами, 2 часа после родов, а тоже через 7 очередных дней. Секрет молочной железы брали от этих коров 2 часа после родов и ежедневно 7 дней. Уровень антител в сыворотке крови и молозиве исследовали тестом ID. Констатировали корреляцию между уровнем титра антител в сыворотке крови и молозиве, а тоже периодом их сохранения в молозиве. Средний титр в молозиве является 8-кратно выше титра в сыворотке крови беременных коров, определенного на 2 месяца перед родами.

EN

In 17 serologically positive cows, aged 4—9 years, the titres of BLV-antibodies in blood serum have been estimated eight weeks before delivery, two hours post partum, and then within seven succeeding days. The secretion of mammary gland was collected from these cows two hours post partum, as well as every day during next week. Both blood serum and colostrum were examined by means of ID test. It has been stated a relationship between the titres of BLV-antibodies in blood sera and in colostrum, as well as between the titre and the survival time of the antibodies in colostrum. The mean titre of antibodies in colostrum occurred to be about eight times higher than that in blood serum of pregnant cows, two months before delivery.

6

Effect of infection with bovine immunodeficiency virus and-or bovine leukaemia virus on lymphocyte blastogenesis to mitogens in sheep

63%

Stec J. , Kuzmak J.

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nr 1

EN

Lymphocyte blastogenesis was determined by ³H-thymidine incorporation assay after stimulation of the cell cultures with mitogens: Concanavalin A, phytohaemagglutitin, and pokeweed mitogen. BIV and/or BLV infection were associated with significant changes in lymphocyte blastogenesis to mitogens. The highest responses to all three mitogens were shown by 3 to 4.5 months after infection when compared to control animals, and thereafter were depressed at the end of the experiment Stimulated lymphocytes revealed in 20 - 50 fold increase in blastogenesis over background based on the unstimulated cells. These results demonstrated that a function impairement of lymphocytes can be observed in the course of BLV and/or BIV infection.

7

Evidence for interspecies transmission of small ruminant lentiviruses in sheep and goats in Poland

51%

Olech M. , Croise B. , Kuzmak J. , Valas S.

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nr 2

165-168

EN

A fragment of 394 bp spanning the V1-V2 variable region of small ruminant lentiviruses (SRLV) env gene was amplified by nested-PCR from peripheral blood leukocytes of 27 sheep and seven goats from five Polish flocks. PCR products were then subjected to analysis by the heteroduplex mobility assay. SRLV found in six sheep were more similar to caprine arthritis-encephalitis virus, but no viruses closely related to maedi visna virus were found in the infected goats. However, in one sheep dual infection with SRLV belonging to both A and B group was found. The results showed that interspecies infections of SRLV occur in animals living under natural conditions.

8

Serological survey for bovine immunodeficiency virus in dairy cattle from Poland

51%

Rola-Luszczak M. , Kozaczynska B. , Kuzmak J.

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nr 4

EN

A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seropositive animal, was 82.6%. To demonstrate the correlation of BIV and bovine leukemia virus infection, all sera were analysed for BLV antibodies and there was only a slight association between both infections. Overall, these results show that BIV infection is present in dairy cattle in Poland at a prevalence rate found in other European countries.

9

Effects of ochratoxin A, for DNA repair in cultures of swine lymphocytes and kidney cells

51%

Stec J. , Rachubik J. , Kuzmak J.

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nr 4

10

Application of non-radioactive method of DNA detection in the diagnosis of bovine leukaemia virus infection

51%

Kuzmak J. , Skorupska A. , Moussa A. , Grundboeck J.

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tom 37

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nr 1

11

Determination of circulating immune complexes in the serum of cattle affected with clinical or subclinical forms of leukosis

45%

Kuzmak J. , Grundboeck-Jusko J. , Bicka L. , Jedrzejewska M. , Kozaczynska B.

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nr 2

12

Występowanie CAEV w stadach kóz wschodniej Polski

32%

Gruszecki T. M. , Olech M. , Kuzmak J. , Bagnicka E. , Szymanowska A. , Szczepaniak K. , Gregula-Kania M. , Tomczuk K. , Bojar W.

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nr 08

EN

The aim of the study was to analyze the occurrence of caprine arthritis encephalitis virus (CAEV) in white improved goats, colored improved goats, Saanen goats, Alpine goats and Boer goats, raised in three regions of Poland. The analysis of CAEV infection levels was carried out in goat herds from the provinces in mid-Eastern Poland. The analysis included four goat herds. In total, they encompassed 251 herds of the following breeds: white improved goat, colored improved goat, Saanen goat, Alpine goat and Boer goat. The identification of the infected animals and the assessment of the epidemiological condition of the herds was carried out using serological analysis of blood serum samples. The assay of CAEV-specific antibodies in blood serum was performed using ELISA test. As a result of the observations it was established that with regards to the provinces, the prevalence level ranged from 35.40% in Mazowieckie province to 81.10% in Lubelskie province, and the average infection rate of animals in the studied region was 51.60%. As far as the breeds are concerned, it was found that the observed infection rate of the Saanen goat population was significantly higher (p ≤ 0.05) than the rates for improved white goat (48.90%) and Alpine goat (50.00%). In relation to the colored improved goat, the calculated statistical difference was greater (p ≤ 0.01), and the percentage of infected animals was 32.10%. The analysis of the infection rate in the Boer goat herd revealed that 66.70% of animals are seropositive, and the statistical analysis did not show any significant differences in prevalence compared to other studied breeds. To sum up, it should be noted that the improvement of the situation may be attributed to consistent examination of goats and development of CAEV-elimination programs for herds. When creating programs aimed at the elimination of the virus it is also important to remember to raise the awareness of the farmers with regards to the detrimental effect of CAEV infections.