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1

Wplyw preparatow TFX-Polfa i lewamizolu na reaktywnosc immunologiczna swin w przebiegu amyloidogenezy

100%

Rotkiewicz Z.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

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1995

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tom 22,Suppl.B

1-59

PL

W przebiegu doświadczalnej amyloidozy u świń, wywołanej domięśniowym podawaniem kazeinianu sodu, określono wpływ lewamizolu i preparatu „TFX-Polfa" na reaktywność układu immunologicznego w zakresie nieswoistej i swoistej odporności komórkowej i humoralnej. Tę ostatnią indukowano żywą szczepionką wirusa chA. Z przeprowadzonych badań wynika, że w okresie przedamyloidowym następuje wzrost, a w fazie amyloidowej spadek wartości wszystkich badanych parametrów, tj. liczby limfocytów T i B, ich aktywności metabolicznej, aktywności fagocytarnej leukocytów, poziomu dopełniacza, lizozymu i przeciwciał seroneutralizujących wirus chA. Immunorekonstrukcyjny wpływ badanych immunostymulatorów był większy w drugiej fazie - amyloidowej i przejawiał się zmniejszeniem odkładania złogów amyloidu w narządach, co oznacza, że wymierna skuteczność preparatu „TFX-Polfa" była wyższa niż lewamizolu.

EN

The effect of levamisole and „TFX-Polfa" preparation on the immunological system reactivity concerning cellular and humoral immunity, specific and non-specific, was determined in the course of experimental amyloidosis induced by means of sodium caseinate injected intramuscularly. The humoral immunity was induced using attenuated ADV vaccine. The research results show that the values of all examined parameters, ie the number of T and В lymophocytes, their metabolic activity, phagocytic activity of leucocytes, the levels of complement, lysozyme and seroneutralising antibodies for ADV increased at the stage of pre-amyloidosis and decreased at that of amyloidosis. The immunoreconstructive effect of examined immunostimulators was bigger in the phase of amyloidosis and was revealed by decrease in deposit accumulation in the organs; the efficiency of „TFX-Polfa" preparation was higher than that of levamisole.

2

Wpływ dożołądkowego podawania roztworu HCl prosiętom oseskom na obraz patomorfologiczny błony śluzowej i zachowanie się mikroflory żołądka i dwunastnicy

63%

Rotkiewicz T. , Rotkiewicz Z.

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tom 47

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nr 08

EN

The investigations were carried out on 60 piglets divided into 5 groups of age from 6 h to 14 days. The piglets of experimental groups (C, D, E) were intragastrically administered 0.18% solution of HCl. The HCl was administered on day 3 to group C, 7th day to group D and 10th day to group E daily up to 14 days. At 14th day all the piglets were slaughtered and tissues from stomach and duodenum were collected and processed for macroscopic histopathologic, histochemical, microbiological and for scanning and transmission electron microscopic study. The short time (group E) administration of HCl produced no morphological changes in the mucous membranes of the stomach and duodenum, but the strong time (groups C and D) administration of HCl produced deformation and desquamation of the epithelium of duodenal villi. There was increased production of mucin and decreased activities of lactic and succinate dehydrogenases on the surface of mucous membranes and endocrine cells were also stimulated. Microbial counts of stomach showed decreased counts of haemolytic strains of Escherichia coli, Streptococcus, Micrococcus and increased counts of Saccharomyces were observed in experimental piglets.

3

Effect of Methisoprinol and KLP-602 on the development of immunocompetent organs and selected biochemical indices of the allantoic fluid of chicken embryos

51%

Malaczewska J. , Rotkiewicz Z. , Siwicki A. K.

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nr 3 Suppl.

4

Laktoferyna - mechanizmy dzialania przeciwwirusowego

51%

Malaczewska J. , Rotkiewicz Z. , Siwicki A. K.

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nr 10

1104-1107

EN

Lactoferrin (LF) is an iron-binding protein from the transferrin family present in mucus secretions such as milk, tears, saliva, seminal and vaginal fluids and also found in several organs and blood. It is well known for having antibacterial, immunomodulatory and anti-inflammatory properties. Lactoferrin is also capable of inhibiting the replication of both DNA- and RNA-viruses. Studies indicate that LF inhibits infection of the host cell by directly binding to virus particles or by binding to target cell molecules that the virus uses as a receptor or co-receptor. Additionally, LF regulates the antiviral response of the immune system through stimulating cells which play an important role during the early phases of viral infection, e.g. natural killer cells, monocytes, macrophages and granulocytes. The use of lactoferrin as an antiviral drug has a promising future, especially in cases of patients with immunosupression.

5

Wplyw zywienia prosiat w okresie okoloodsadzeniowym pasza zakwaszana HCL na obraz morfologiczny i sklad mikroflory zoladka i dwunastnicy

51%

Rotkiewicz T. , Rotkiewicz Z. , Przala F.

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nr 06

257-259

EN

The study was carried out on 28 days old piglets divided into two groups of forty each. One group received fodder supplemented with exogenous 0.18% HCl and a control group was fed on unacidified fodder. The piglets were slaughtered at the age of 35, 42, 49 and 56 days. Distinct morphological lesions were found in the gastric mucosa and duodenum of piglets fed on acidified fodder and slaughtered at 49—56 days of age. Histopathological changes included the demaged duodenal villi with cellular infiltration and an increased amount of acid mucin. The gastrointestinal microflora showed a decrease in the number of E. coli and an increase of yeast; the number of Lactobacillus strains remained unchanged.

6

Porownanie testow: transformacji blastycznej, hamowania migracji leukocytow oraz alergicznego testu skornego u cielat sztucznie zakazonych wirusem BHV-1

51%

Wisniewski J. , Trybala E. , Rotkiewicz Z. , Grabowska G.

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tom 49

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nr 03

143-136

EN

In calves at the age of 2—3 months experimentally infected with BHV-1 clinical signs, humoral and cellular responses and the intensity of delayed skin hipersensitivity were examined. The examinations were performed from 3 to 56 day post infection, e.g. at early period of infection. It was found that apart from typical clinical signs and humoral response to BHV-1 infection, cellular immune response measured by the test of blastic transformation of leukocytes appeared by about 7 days before humoral immune response and that it persists only up to the day 21 post infection. In the leukocyte migration tests, leukocyte inhibition was noted at the day 7 and from that day since the end of observation acceleration of leukocyte migration was noted. A delayed skin hipersensitivity measured by allergic skin test appeared between day 21 and 28 post infection.

7

Effect of Lactobacillus casei colonization on the immune system of the alimentary tract in gnobiotic piglets

51%

Rotkiewicz T. , Rotkiewicz Z. , Bomba A.

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nr 3

8

Effect of Lactobacillus acidophilus and Bifidobacterium sp. on the course of Cryptosporidium parvum invasion in new-born piglets

51%

Rotkiewicz T. , Rotkiewicz Z. , Depta A. , Kander M.

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nr 2

EN

The investigations were carried out on suckling piglets, naturally infected with Cryptosporidium parvum. Starting from the 3rd day, two experimental groups were given intragastrically Lactobacillus acidophilus culture (1 x 10⁸ cells) or Bifidobacterium sp. culture (1 x 10⁸ cells) per day. Cryptosporidium was present in the stomach, microvillus brush border of the jejunum, ileum and colon. These findings were associated with severe atrophy of the villi and mild lymphoid infiltration in the lamina propria. Haematological and biochemical tests in piglets showed significant differences including a reduced haemoglobin level, a decrease in the erythrocyte count, an increase in the haematocrit value, leukocyte count, total protein value and in alkaline phosphates and LDH activity. Bacterial flora was less abundant in piglets receiving Lactobacillus acidophilus and Bifidobacterium; no haemolytic E.coli rods were isolated. After daily administration of the bacteria, the diarrhoea was moderate, lasting up to 2 days, and the number of oocysts in faeces was significantly lower. This suggests that Lactobacillus acidophilus and Bifidobacterium sp. may help to control C. parvum infection in new-born piglets.

9

Cellular response of calves experimentally infected with IPV or IBR strains of bovine herpesvirus type 1 [BHV1]

51%

Wisniewski J. , Swiecicka-Grabowska G. , Rotkiewicz Z. , Wojcik R.

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1999

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tom 02

10

Serological and allergic response of calves experimantally infected with IPV or IBR strains of bovine herpesvirus type 1 [BHV1]

45%

Wisniewski J. , Rutherford M. , Swiecicka-Grabowska G. , Rotkiewicz Z. , Wojcik R.

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1996

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tom 24

93-100

EN

Eight ten-week old calves, seronegative to BHVl,were divided at random into 2 groups of four. One group was infected with the Cooper (IBR) strain, while the other group was treated with the 509/89 (IPV) strain. Their humoral immunologic response to the virus was cross tested using the seroneutralisation (SN) test (with both strains) and an immunoenzymatic (IBR - EIA, Svanova) test. However, allergic skin tests using two preparations that were obtained from the above mentioned strains,were applied in the same way as in a tuberculin test. The tests showed that immunogenic properties of the used strains are similar (the mean titres of antibodies in both groups did not differ significantly) but they differ as far as antigenic features are concerned. Lower titres of antibodies were detected with the 508/89 (IPV) strain than with the Cooper (IBR) strain. Humoral immunologic response, tested with EIA, was positive in calves infected with the (IPV) strain (on the 35th day p.i.) and negative in the Cooper (IBR) strain infected group. Allergic tests using „homo- and heterological" preparations, showed that a delayed type of hypersensitivity (DTH) of skin developed in animals from the IPV group on day 35 p.i. (heterological preparation), whereas on day 56 p.i. in the case of the homological preparation. The DTH of skin was maintained up to the end of the experiment. Calves from the IBR group, tested for skin allergy with both preparations, reacted only sporadically positively. The possible reasons for the confirmed differences have been discussed.

PL

Osiem 10-tygodniowych cieląt, seroujemnych wobec BHV1, podzielono losowo na dwie grupy po 4 osobniki i zakażono jedne szczepem Cooper (IBR), drugie szczepem 508/89 (IPV). Ich odpowiedź humoralną na wirus badano krzyżowo odczynem seroneutralizacji przy użyciu obu szczepów i immunoenzymatycznie, a odczyn alergiczny skóry dwoma preparatami uzyskanymi we własnym zakresie z wyżej wymienionych szczepów, które aplikowano jak w badaniu tuberkulinowym. Wykazano, iż właściwości immunogenne badanych szczepów są podobne (zbliżone poziomy średnich mian przeciwciał w obu grupach), natomiast różnią się w zakresie antygenowym szczepem IPV (508/89), użytym jako antygen diagnostyczny, wykrywano bowiem niższe miana niż stosując w tym celu szczep Cooper. Odpowiedź humoralna badana odczynem EIA byla pozytywna u zwierząt zakażonych szczepem IPV; nie wykazano natomiast serokonwersji u cieląt pozostałej grupy. Badaniem alergicznym z zastosowaniem preparatów „homo- i heterologicznego" wykazano, że nadwrażliwość skóry typu późnego na preparat heterologiczny rozwinęła się u osobników grupy IPV już w 35., na preparat homologiczny zaś dopiero w 56. dniu po zakażeniu. Wykazana NTP utrzymywała się u reagentów do końca eksperymentu. Cielęta grupy IBR badane obydwoma preparatami reagowały sporadycznie dodatnio. Omówiono możliwe przyczyny stwierdzonych różnic.

11

Effect of laser biostimulation on cell proliferation in the healing of cutaneous surgical wounds in pigs

38%

Chyczewski M. , Rozicka A. , Mikolajczyk A. , Rotkiewicz T. , Holak P. , Jalynski M. , Rotkiewicz Z.

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nr 2

217-221

EN

The objective of this study was to investigate the effect of low-level laser therapy (LLLT) on the proliferation of epithelial and connective tissue cells in the healing of incised cutaneous wounds in pigs. The experiment was conducted on 12 young pigs divided into four groups. Group I (undamaged skin) and group III (damaged skin) served as control. Group II - pigs with undamaged skin and group IV - pigs with incised wounds in the dorsal area were subjected to laser irradiation. Laser biostimulation was carried out using a CTL 1106 MX semiconductor laser in the continuous wave mode of operation at a wavelength of 810 nm and a maximum output of 100 mW. Following three weeks of observation, skin specimens were collected for histopathological analysis (HE), immunohistochemical detection of PCNA, and determination of apoptosis (TUNEL) and presence of mast cells (toluidine blue staining). Laser irradiation administered at E=8 J/cm for 1 min over a period of three weeks accelerated the proliferation of stratum basale cells, stimulated fibroblast proliferation, increased the number of mast cells in the wound area, and inhibited apoptosis in cells participating in the skin regeneration process.

12

Aktywnosc bakterio-, wiruso-, grzybo- i pasozytniczobojcza preparatow Lysoformin 3000, Trichlorol i Desoform

32%

Wisniewski J. , Swiecicka-Grabowska G. , Rotkiewicz Z. , Rotkiewicz T. , Wisniewska M. , Bomba G. , Michalski M. , Szelagiewicz M. , Bah M.

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nr 12

411-414

13

Skutecznosc dezynfekcyjna preparatow Lysoformin 3000, Desoform i Trichlorol w zakladach przetworstwa miesnego i obiektach weterynaryjnych oraz badanie toksycznosci ostrej tych preparatow

32%

Rotkiewicz T. , Wisniewska M. , Bomba G. , Wisniewski J. , Rotkiewicz Z. , Swiecicka-Grabowska G. , Szteyn J. , Jozwik E. , Radkowski M.

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nr 11

372-374