Wyniki wyszukiwania - Biblioteka Nauki

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Postprandial decrease in LDL-cholesterol in men with metabolic syndrome

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Skoczyńska A. , Turczyn B. , Wojakowska A. , Kreczyńska B. , Skoczyńska M. , Wojtas K.

Open Medicine

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2014

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tom 10

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nr 1

EN

Background: In some epidemiological studies, blood lipids are determined at non-fasting state, which may impact cardiovascular risk estimation. The aim of this study was to evaluate postprandial LDL-C changes in men with newly diagnosed metabolic syndrome (MetSy). Methods: 36 male patients were examined: 12 men with and 24 men without MetSy. The fat tolerance test was performed before and after a three-month hypolipidemic treatment. Serum lipids were measured using routine methods, lipid peroxides (LPO) colorimetrically, apoli- poproteins A-I, B, and hsCRP immunoturbidimetrically. Results: The postprandial increase in triglycerides was associated with a decrease in LDL-C and a small decrease in apo B. In men with MetSy, the mean change in LDL-C (-19.5 ± 2.3 mg/dl) was greater than in healthy men (-5.7 ± 3.8 mg/dl). All lipid changes (ΔTG, ΔLDL-C and ΔLPO) were linearly dependent on the postprandial non-LDL-choles- terol. After three months of hypolipidemic treatment, in all men with MetSy, the apoB/apoA-I ratio remained the same as before the therapy. Conclusion: In men diagnosed with MetSy, postprandial decreases in LDL-cholesterol may cause underestimation of cardiovascular risk. After three months of hypolipidemic treatment, there was only a partial reduction in this risk, as the apoB/apoA-I ratio remained the same.

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The effect of polyunsaturated fatty acids on cardiovascular risk factors and urinary mercury in workers employed at electrolytic production of chlorine

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Skoczyńska A. , Cyran M. , Turczyn B. , Wojakowska A. , Smyk B. , Skoczyńska M.

International Journal of Occupational Medicine and Environmental Health

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2021

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tom 34

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nr 4

551-564

EN

ObjectivesWhile inorganic mercury is being gradually withdrawn from industry, environmental exposure to mercury is recognized as one of the greatest present toxicological problems. The aim of this study was to evaluate the effect of polyunsaturated fatty acids (PUFAs) supplementation on selected cardiovascular risk factors and the urinary mercury (Hg-U) concentration in workers occupationally exposed to mercury vapor.Material and MethodsOverall, 38 workers of an electrolyzer hall (Hg-U: 46.6±35.7 μg/g creatinine) and a control group of 60 employees not exposed to Hg (Hg-U: 4.3±15.5 μg/g creatinine) were included in a clinical cross-over study. Clinical and laboratory tests were carried out 4 times: before and after a 3-month period of PUFAs supplementation (1000 mg daily), then after a 3-month break, and then after another 3-month period of PUFAs supplementation.ResultsThe baseline heart rate (HR) and serum triglyceride levels were higher in the Hg-exposed workers than in the controls, whereas systolic blood pressure (SBP) and cholesterol (C) levels exceeded normal values in both groups. There was a positive correlation between high-density lipoprotein 3 cholesterol (HDL3-C) and Hg-U levels. The PUFAs use was associated with a decrease in both HR and SBP. After the first stage of supplementation, a decrease in the Hg-U concentration was observed. In a multivariate logistic regression model, decreases in Hg-U were associated only with exposure to mercury; ORΔHg = 0.562 (95% CI: 0.323–0.979), p < 0.042. After the second 3-month period of PUFAs supplementation, a significant association between HDL3-C and a Hg-U decrease was shown: OR HDL3 = 1.222 (95% CI: 1.01–1.46), p < 0.033.ConclusionsIn the workers exposed to mercury vapor, PUFAs supplementation led to some beneficial effects on HR and SBP. The first stage of supplementation was associated with a decrease in Hg-U in which HDL3 metabolism probably plays an important role.

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Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma

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Jelonek K. , Wojakowska A. , Marczak L. , Muer A. , Tinhofer-Keilholz I. , Lysek-Gladysinska M. , Widlak P. , Pietrowska M.

Acta Biochimica Polonica

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2015

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tom 62

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nr 2

265-272

EN

Exosomes are membrane vesicles of endocytic origin that participate in inter-cellular communication. Environmental and physiological conditions affect composition of secreted exosomes, their abundance and potential influence on recipient cells. Here, we analyzed protein component of exosomes released in vitro from cells exposed to ionizing radiation (2Gy dose) and compared their content with composition of exosomes released from control not irradiated cells. Exosomes secreted from FaDu cells originating from human squamous head and neck cell carcinoma were analyzed using LC-MS/MS approach. We have found that exposure to ionizing radiation resulted in gross changes in exosomal cargo. There were 217 proteins identified in exosomes from control cells and 384 proteins identified in exosomes from irradiated cells, including 148 "common" proteins, 236 proteins detected specifically after irradiation and 69 proteins not detected after irradiation. Among proteins specifically overrepresented in exosomes from irradiated cells were those involved in transcription, translation, protein turnover, cell division and cell signaling. This indicated that exosomal cargo reflected radiation-induced changes in cellular processes like transient suppression of transcription and translation or stress-induced signaling.

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Panel of serum metabolites discriminates cancer patients and healthy participants of lung cancer screening - a pilot study

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Roś-Mazurczyk M. , Wojakowska A. , Marczak Ł. , Polański K. , Pietrowska M. , Polanska J. , Dziadziuszko R. , Jassem J. , Rzyman W. , Widlak P.

Acta Biochimica Polonica

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2017

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tom 64

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nr 3

513-518

EN

Introduction. Blood biomarkers may support early diagnosis of lung cancer by enabling pre-selection of candidates for computed tomography screening or discrimination between benign and malignant screening-detected nodules. We aimed to identify features of serum metabolome distinguishing individuals with early-detected lung cancer from healthy participants of the lung cancer screening program. Methods. Blood samples were collected in the course of a low-dose computed tomography screening program performed in the Gdansk district (Northern Poland). The analysis included 31 patients with screening-detected lung cancer and the pair-matched group of 92 healthy controls. The gas chromatography coupled to mass spectrometry (GC/MS) approach was used to identify and quantify small metabolites present in serum. Results. There were several metabolites detected in the sera whose abundances discriminated patients with lung cancer from controls. Majority of the differentiating components were downregulated in cancer samples, including amino acids, carboxylic acids and tocopherols, whereas benzaldehyde was the only compound significantly upregulated. A classifier including nine serum metabolites allowed separation of cancer and control samples with 100% sensitivity and 95% specificity. Conclusions. Signature of serum metabolites discriminating between cancer patients and healthy participants of the early lung cancer screening program was identified using a GC/MS metabolomics approach. This signature, though not validated in an independent dataset, deserves further investigation in a larger cohort study.

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Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy

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Roś-Mazurczyk M. , Wojakowska A. , Marczak Ł. , Polański K. , Pietrowska M. , Jelonek K. , Domińczyk I. , Hajduk A. , Rutkowski T. , Składowski K. , Widłak P.

Acta Biochimica Polonica

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2017

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tom 64

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nr 1

189-193

EN

Radiotherapy causes molecular changes observed at the level of body fluids, which are potential biomarker candidates for assessment of radiation exposure. Here we analyzed radiotherapy-induced changes in a profile of small metabolites detected in sera of head and neck cancer patients using the gas chromatography coupled with mass spectrometry approach. There were about 20 compounds, including carboxylic acids, sugars, amines and amino acids, whose levels significantly differed between pre-treatment and post-treatment samples. Among metabolites upregulated by radiotherapy there was 3-hydroxybutyric acid, whose level increased about three times in post-treatment samples. Moreover, compounds affected by irradiation were associated with several metabolic pathways, including protein biosynthesis and amino acid metabolism.