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Characterization and localization of 11S-type storage proteins during olive seed development and germination

100%

Jimenez J. C. , Alche J. D. , Wang W. , Rodriguez-Garcia M. I.

nr 3 suppl.

Observations on microtubules and nuclei motility in the pollen tube of olive [Olea europaea L.]

100%

Rodriguez-Garcia M. I. , Alaoui M. M. , De la Flor Diaz J. , Del Carmen Fernandez M.

nr 1

In order to study microtubule organization during pollen germination and pollen tube growth in Olea europaea, we applied immunofluorescence microscopy using mouse monoclonal antibody against α-tubulin as primary antibody and FITC-conjugated goat antimouse IgG as secondary antibody. DAPI enabled observation of the vegetative nucleus entering the emerging pollen tube before the generative cell. The latter then overtakes the vegetative nucleus once both are inside the pollen tube. The generative cell remains ahead of the vegetative nucleus until it is finally divided into two gametes. This cell division occurs when the generative cell is close to the tip of the pollen tube. Possible connections between microtubules and nuclear migration in the pollen tube are discussed.

Ole e 10, a 1,3-β-glucanase from olive pollen, co-localizes to callose during pollen tube growth

88%

Suarez C. G. , Barral P. , de Dios Alche J. , Rodriguez R. , Rodriguez-Garcia M. I.

tom 47

nr 1

Temporal and spatial gene expression of Ole e 3 allergen in olive [Olea europaea L.] pollen

88%

De D. A. J. , Cismondi I. A. , Castro A. J. , Hamman-Khalifa A. M. , Rodriguez-Garcia M. I.

nr 1

This paper describes the distribution of Ole e 3 allergen and its transcripts in the developing anther and in mature olive pollen. Northern blot and RT-PCR analyses over the course of pollen development show that Ole e 3 transcripts accumulate exclusively in mature pollen. This gene therefore corresponds to a "late gene." The sequences amplified by RT-PCR display high identity with those already reported for Ole e 3, including two Ca2+-binding motifs. Immunoblot analysis of the allergen shows that Ole e 3 accumulates during the same stage as the corresponding transcripts, suggesting a transcriptional regulation mechanism for the expression of the gene. Finally, the use of transmission electron microscopy techniques has shown that (a) the allergen is located mainly in the vicinity of membrane systems and in the aperture regions of the mature pollen grain, and (b) Ole e 3 transcripts are detectable after using in situ RT-PCR. These data are significant clues to the biological roles of the protein in olive pollen biology. Such putative functions are discussed.