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2 Acta Physiologiae Plantarum

2 2013

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Effects of dynamic changes of nutrients on adventitious roots growth and periplocin accumulation in culture of Periploca sepium Bunge

100%

Yin S. , Gao W. , Wang J. , Zhang J. , Man S. , Jing S. S. , Liu H.

Acta Physiologiae Plantarum

2013

tom 35

nr 11

In this study, the dynamics of biomass production, accumulation of periplocin, medium conditions and consumption of carbon, nitrogen and phosphate were investigated in adventitious roots culture of Periploca sepium in shake flasks over a period of 4 weeks. The biomass reached the maximum peak on day 24 (2.46 and 0.213 g of fresh and dry weight, respectively). Similarly, periplocin production got to a peak of 0.083 mg g−1 on day 24, simultaneously. PH in medium had a decrease tendency at the beginning and then remained stable around 4.0, however, EC declined during the whole culture with the nutrients consumed. Sucrose was almost used up on the first 12 days which led to the increase in glucose and fructose. In case of nitrogen, consumption of ammonium is faster than nitrate at the beginning. Phosphate was almost consumed during the first 8 days. Based on the nutrients consumption, the adding time of nutrients (1/2 MS medium and 30 g L−1 sucrose) was investigated and it obtained highest content of periplocin (0.106 mg g−1) and yield (0.513 mg L−1) on day 12.

Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

100%

Dong Y. , Gao W.-Y. , Man S. , Zuo B. , Wang J. , Huang L. , Xiao P.

Acta Physiologiae Plantarum

2013

tom 35

nr 05

Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90º at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90º than the other ones. Aeration rates impacted on the growth ratio, the specific O2 uptake rate (SOUR) of ginseng cells were investigated. 0.4 vvm was selected as the optimal aeration rate with a dry weight of 6.45 g L-1. The specific O2 uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.