With the recent and continuing discovery of further cryptic bat species, it is essential to find morphological species discriminating characters. Pipistrellus pipistrellus (common pipistrelle) and Pipistrellus pygmaeus (soprano pipistrelle) have been recognized as separate species since 1997, but no reliable morphological species discriminating trait has yet been found. The most commonly used morphological species discrimination traits are ‘wing vein’ pattern and shape and color of the penis, but these have not been validated on sets of genetically identified specimens. The baculum (os penis) has long been used successfully in species discrimination in bats and other mammals. In this study, we tested the reliability of the established traits and demonstrated how to reliably separate the common pipistrelle and the soprano pipistrelle by simple baculum measurements. The bacula of museum specimens of these two species and of Pipistrellus hanaki were imaged with high-resolution microCT. Several measurements were taken on the size-calibrated volume images, and their value for species discrimination was tested by discriminant analysis with leave-one-out cross validation. We showed that P. pipistrellus and P. pygmaeus specimens can be discriminated by measuring the projected length, height, and width of the baculum (n = 48; all but one classified correctly). Geometric morphometrics was used to analyze and locate variations in baculum shape. Principal component analysis of baculum variation was not sufficient to separate these species. Most of the interspecific variation in baculum shape can be found in the proximal third (the base) of the baculum, and most individual variation can be observed in lateral view, especially in the dorsoventral curve. Quantitative details of morphology are becoming more important to distinguish cryptic species and understand their phylogeographic distributions. The simple baculum measurements can be used to classify single specimens and could be taken without microCT, on a resected baculum.
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The purpose of this paper is to evaluate the performance of the TSMP-assisted Digisonde (TaD) topside profiling technique. We present systematic comparisons between electron density profiles and TEC parameters extracted from TaD model with (a) CHAMP-derived TEC parameters, (b) CHAMP reconstructed profiles, (c) ground based GPSderived TEC parameters, and (d) profiles reconstructed from RPI/ IMAGE plasmagrams. In all cases, TaD follows the general trend of plasmaspheric observations derived from the above datasets. Especially during storm cases, TaD shows remarkable agreement with the variations of the ground based GPS-derived TEC parameters. Overall, the comparison results shows that TaD method can be adopted by EURIPOS to provide the electron density distribution up to plasmaspheric heights in real-time.
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We apply a model-assisted technique to construct the topside electron density profile based on Digisonde measurements. This technique uses the Topside Sounder Model (TSM), which provides the plasma scale height, O+-H+ transition height, and their ratio Rt = HT/hT, derived from topside sounder data of Alouette and ISIS satellites. The Topside Sounder Model Profiler (TSMP) incorporates TSM and uses the model quantities as anchor points for the construction of topside density profiles. TSMP provides its model ratios with transition height and plasmaspheric scale height. The analysis carried out indicates that Digisonde derived F-region topside scale height Hm is systematically lower than one derived from topside sounder profiles. To construct topside profiles by using Hm, a correction factor of around 3 is needed to multiply the neutral scale height in the ?-Chapman formula. It was found that the plasmaspheric scale height strongly depends on latitude and its ratio with the F-region scale height expresses large day-to-day variability.