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tom Vol. 27, no. 3
175-189
EN
A non-stationary environmental noise fluctuations can be treated as some composition of local state strips having arbitrary fluctuating pattern in a locally stationary time period. Its non-stationary characteristics can be seen in the form of its changing stage from a locally stationary state to another one by means of its temporal change of probability distribution. For a non-stationarity with continuously slow temporal change, the noise evaluation method is derived by considering the temporal change of moment statistics or distribution parameters. On the other hand, for a non-stationarity with stepwise rapid variation like an ON/OFF operation of machine, the noise evaluation method is derived by considering in principle the occurring probability of each of locally stationary state based on the mutually exclusive property. In the experimental consideration, the above contrastive two evaluation methods have been applied to actual cases with slowly changing environmental noise and rapidly changing machine noise.
EN
Retinal lipids of crayfish, kept at 4°C under continuous darkness for 3 weeks, consisted mainly of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS) were minor contributors. PI, involved in the phototransduction cascade, never reached greater concentrations than 7% of the total. High concentrations of polyunsaturated fatty acids (PUFA) such as 20:4n-6, 20:5n-3 and 22:6n-3 (DHA, docosahexaenoic acid) were present in PC, PE and PS, but scarce in SM and PL In retinae of crayfish kept at 4°C in darkness for 3 weeks and then exposed to white light (6 h; ca. 4,500 lx), SM and PS remained seemingly unaffected. PC, however, significantly decreased within 10 min to 65% of the initial value and 50% at 180 min. To study the reduction of PC, lipids of retinae suspended in physiological solution with/without phospholipase C (PLC) and phospholipase A2 (PLA2) inhibitors such as DMDA (=DEDA), manoalide, ET-I8-OCH3, and U-73122 were measured. Only free fatty acids (FFA) of retinae with inhibitors of PLA2 like DMDA and manoalide decreased. Retinae irradiated by white light for 3 h displayed a significant reduction of PC, compared with those that had remained in continuous darkness. However, the PC of retinae with PLA2-inhibitors was not decreased by light. Our results provide evidence that not only photoreceptor cell PLC, but also PLA2 is activated by light.
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