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RU
Цель работы состояла в определении MIC линкомицина содержащегося в противоспирохеточных препаpатах, для штаммов Treponema hyodysenteriae, изолированных в Польше, а также в оценке профилактической и лечебной эффективности Linco-Spectin в борьбе с дизентерией свиней (DS), MIC 90 линкомицина для Т. hyodysenteriae составлял 20 µг/мл. Linco-Spectin 100 применили 2-кратно профилактически в течение 7—10 дней в корме в дозе 5 мг/кг М.Т., что предотвращало у 1040 свиней заболевание DS, Linco-Spectin 100, употребленный для лечения в течение 7 дней в дозе 10 мг/кг м.т. на 2 фермах (1057 свиней), вызывал исчезновение клиничеемких симптомов через 24—48 часов. На этих фермах не наблюдалось возвращение болезни. Похожие результаты получили на 6 фермах у 3253 свиней, где лечили больных DS свиней препаратом Linco-Spectin 44 в дозе 88 ppm в корме 5 дней. При употреблении же метронидазола отмечали повторные заболевания в стаде. Хорошие результаты профилактического употребления метронидазола против DS получили при дозе 20 мг/кг м.т. после ввода в течение 7 очередных дней. MIC для метронидазола составлял 0,1—1,5 µг/мл. Через некоторые время на тех же самых формах MIC для метронидазола составлял уже 15 µг/мл. Доза 20 мг/кг м.т., употребляемая в лечении, не предотвращала повторных заболеваний DS в стаде. Ронидазол в дозах, рекомендуемых проузводителем, был не эффективен в борьбе с DS MIC 220 составлял 7,5 µг/мл. Результаты работы внушаают, что Linco-Spectin является эффективным средством для предотвращения и лечении дизентерии свиней в Польше.
EN
The purpose of the work was to determine MIC of lincomycin with respect to the strains of Treponema hyodystenteriae isolated in Poland and assess the prophylactic and therapeutic effect of Linco-Spectin in the control of swine dysentery (SD). It was found that MIC of lincomycin regarding T. hyodysenteriae was 20 µg/ml. Linco-Spectin 100 was being administered for 7—10 days at the rate of 5 mg/kg of body weight; the antibiotic protected the pigs under study (1040) against SD. Linco-Spectin used for the treatment of 1057 pigs (10 mg/kg) for 7 days caused the cessation of clinical signs within 24-48 hours. No recurrence of the disease was observed. Similar results were noted in other six farms including 3253 animals, where the pigs were cured with Linco-Spectin 44 (88 ppm in fodder) for 5 days. In contrast using metronidazole there was noted the recurrence of the disease. Positive results of prophylactic usage of metronidazole was reached at the rate of 20 mg/kg given far 7 consencutive days. MIC of metronidazole was 0.1—1.5 µg/ml. However, after some time MIC of the drug was 15 µg/ml. At the rate of 20 mg/kg of metronidazole used for the treatment did not protect the animals from SD. Ronidazole applied according to the prescriptions was ineffective in the control of SD; its MIC was 7.5 µg/ml. The findings indicate that Linco-Spectin may be an effective drug in the prophylaxis and treatment of swine dysentery.
EN
The experiment was carried out on 20 piglets. The vaccine was being administered per os with fodder to 10 piglets at a dose of 3 ml (1.5 X 1010 ml-1) for 10 days. The number of E. coli in the faeces was determined in 5 piglets prior to and at day 5 and 10 in the course of vaccination and at day 5, 10 and 15 after the vaccination. In the next experiment in addition to the number of E. coli the numbers of Lactabacillus, Streptococcus and Staphylococcus-Micrococcus sp. were also determined. In this experiment the number of bacteria was counted prior to and at day 5 and 10 of the vaccination, and at day 10 after vaccination. At day 10 and 15 after the vaccina­tion there was observed a decrease of E. coli colonies. During and after vaccination an increase of Lactoba­cillus sp. was noticed. No differences in the number of Streptococcus or Staphylococcus-Micrococcus sp. were found in the control and experimental piglets.
EN
The experiments were carried out on 12 piglets, 15 kg of body weight of each, 5 weeks old. Eight animals received 3 ml of an oral vaccine (1.5X10lo/ml) once daily for 10 days. Four animals served as control. The level of specifis E. coli anti О and anti К anibodies was determined in sera and in feces on day 4 and 10 of the vaccination and on day 15. The level of antibodies in sera of the experimental and control groups was similar. In the deces, however, the level of antibody rose as a result of vaccination. Prior to vaccination the mean antibody titres were 1.37 log2, on day 4—2, 12, day 10—3, 20, and day 15—2, 83 log2 which indicated a more than a 50% increase in relation to the prevaccination levels. The serum total protein and immuno­globulin levels in vaccinated and control group of piglets did not differ remaining within the lower physiological limits for healthy animals. Histopathological electronmicro- scope studies of intestinal sections of the immunized animals showed morphologic characteristics of antigen stimmulation. There were no differences, however, between control and immunized animals in histoenzymatic studies (alkaline and acid phosphatase tests). Challenge was carried out on 3 im­munized and 2 non-immunized piglets using a mixture of pathogenic E. coli strains administered directly to the jejunum. The immunized piglets did not show any clinical symptoms throughout the 2 weeks. The control piglets did contract colibaeillosis and one animal died. Colibaeillosis was confirmed by gross lesions and histopathologically.
EN
The purpose of the work was to find out whether alimentary deficiences had any influence on the occurrence of diseases of the sexual organs in aurochs. The level of Ca, P, Na, K, Mg, Zn, Cu, protein and glucose was determined in 34 animals aged from eight months to twenty years. Besides, the content of Ca, P, Na, K, Mg, Fe, Zn, Cu, and Mn was evaluated in hay which was being given to the aurochs in the winter season. The animals were divided into 3 groups: a) Healthy animals without any signs of disease in the genital organs, b) Male aurochs with lesions on the prepuce and phallus noted clinically and at necropsy, c) Aurochs with parasitic invasion (F. hepatica) of the liver, lungs and alimentary tract (roundworms). The studies did not confirm the suggestions that alimentary deficiences influenced the occurrence of lesions in the sexual organs. The lower level of Ca, P and Mg in the serum of the aurochs was attributed to the deficiences of these elements in hay.
EN
The goal of the studies was evaluation of the influence of some parameters of cellular immunity on age and anatomo-pathological lesions in the bison. The results of the studies show an influence of the state of health of the bison as measured in a system of assessing points for various gross pathological lesions, on an importent cellular immunity parameter (reactions level of lymphocytes to mitogen (PHA). The better the ability to mobilize lymphocytes the better the health condition of the bison was found to be (lower amount of anatomopathological points). At the same time a higher mobility of lymphocytes was found in older animals. Results of hematological tests. Which showed higher total lymphocyte and leucocyte numbers (per 1 mm³ of blood) also suggest, a better effectiveness of the immunologic system in older animals.
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