Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Evaluation of a Salmonella strain isolated from honeybee gut as a potential live oral vaccine against lethal infection of Salmonella Typhimurium

100%

Zafar H. , Rahman S. U. , Ali S. , Javed M. T.

nr 2

In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by ampli-fication of the 16S rRNA gene. Furthermore, sequencing of the amplicons confirmed all ten isolates as Salmonella strains. The antigenic cross-reactivity was found maximum between the HB1 (strain isolated from honeybee) antiserum and its antigen with an antibody titer of 1:128, while the HB1 antiserum showed a cross-reactive titer range of 1:8 to 1:64. On the basis of the highest geometric mean titer (GMT) shown by the antiserum of the HB1 antigen, it was selected as the best candidate for a cross-reactive live Salmonella oral antigen. Moreover, the HB1 antigen was used a live oral antigen (1×10¹⁰CFU/ml) in a safety test in rabbits and proved to be avirulent. During the animal trial, three different oral doses of the HB1 live oral antigen were evaluated in four different rabbits’ groups (R1, R2, R3, and R4). The dose number 2 of 0.5ml (two drops orally and repeated after one week) gave the best GMT measured by indirect hemagglutination (IHA) as compared to the other two doses, while R4 group was kept as control. Results of the challenge protection test also validated the efficacy of the double dose of the HB1 live vaccine, which gave the highest survival percentage. Results of this study lay the foundation for a potential cross-reactive live oral Salmonella vaccine that has proved to be immunogenic in rabbits.

Medical image registration in image guided surgery: Issues, challenges and research opportunities

100%

Alam F. , Rahman S. U. , Ullah S. , Gulati K.

2018

tom Vol. 38, no. 1

71--89

Multimodal images of a patient obtained at different time, pre-surgical planning, intra-procedural guidance and visualization, and post-procedural assessment are the core components of image-guided surgery (IGS). In IGS, the goal of registration is to integrate corresponding information in different images of the same organ into a common coordinate system. Registration is a fundamental task in IGS and its main purpose is to provide better visualization and navigation to the surgeons. In this paper, we describe the most popular types of medical image registration and evaluate their prominent state-of-the art issues and challenges in image-guided surgery. We have also presented the factors which affect the accuracy, reliability and efficiency of medical image registration methods. It is not possible to achieve highly successful IGS until all the issues and challenges in registration process are identified and subsequently solved.

Comparative growth kinetic study of Newcastle disease virus, infectious bursal disease virus and avian influenza virus in chicken embryo fibroblast and DF-1 cell lines

89%

Anam S. , Rahman S. U. , Ali S. , Saeed M. , Goyal S. M. , Anam S. , Rahman S. U. , Ali S. , Saeed M. , Goyal S. M.