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EN
The aim of the study was to determine whether non-essential amino acids (NEAA) in form of wheat gluten (WG) have a threonine (Thr)- sparing effect on gut structure in young pigs. A two-factorial experiment was conducted on 12 groups of 6 pigs. Pigs of 12.5 kg initial body weight (BW) were fed for about 20 days diets differing in the Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible Thr · kg–1) and WG level (20.4, 40.4 and 60.4 g of WG protein · kg–1 of respective WG20, WG40 and WG60 diets). After slaughter at about 22.4 kg of BW, samples of duodenum, middle jejunum, ileum and proximal colon were taken for histological analysis. Morphology of all segments was not affected neither by Thr level nor by Thr and WG levels interaction, whereas effect of WG level differed among segments. In the duodenum, villi length and tunica mucosa thickness were decreased by WG60. In the duodenum and proximal colon, crypt depth was greater in WG20 than WG60 and/or WG40 groups, whereas in the ileum was greater in WG60 than WG20 group, and in the middle jejunum it was not affected. Villi length : crypt depth ratio was affected by WG only in the ileum, being greater in WG20 group. Myenteron was thicker in the ileum and proximal colon in WG60 than WG20 and WG40 group, respectively. The results do not evidence a sparing effect of NEAA provided by WG on Thr utilization for maintaining the proper gut structure in young pigs.
EN
This study focuses on the variability of chromosomal location and number of ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis and Lolium perenne cultivars, as well as on identification of rDNA-bearing chromosomes in their triploid and tetraploid F. pratensis × L. perenne hybrids. The rDNA loci were mapped using fluorescence in situ hybridization (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a probe, and F. pratensis genomic DNA as a block. FISH detected variation in the number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis mostly additional signals of 5S rDNA loci occurred, as compared with standard F. pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne genomes approved a higher number of rDNA sites as well as variation in chromosomal rDNA location in L. perenne. A greater instability of F. pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. pratensis and on chromosome 3 in L. perenne are useful markers for these chromosomes in intergeneric Festuca × Lolium hybrids.
EN
A potential source of potato dietary fibre (PDF) is potato pulp, a waste product of the starch industry. The refined potato pulp (PDF preparation) contains 50-60% of DF, 10-20% of starch and 6% of protein (on dry matter basis). PDF consists of about 55% of insoluble (mainly cellulose) and 45% of soluble (mainly pectins) fraction; has low viscosity and relatively high water-holding capacity but lower fecal bulking properties than cellulose. It is intensively fermented in the large intestine, yielding a high amount of acetate and butyrate. Feeding PDF does not affect greatly morphology and morphometry of the intestinal tract. It delays the rate of passage of digesta (as compared with cellulose), reduces fat and protein digestibility in the whole digestive tract and does not affect ileal protein digestibility (as compared with cellulose and pectin). It seems to improve mineral absorption. Few experimental results do not allow to conclude whether PDF decreases blood concentration of triglycerides, total cholesterol or LDL fraction. Data on the effects of PDF on glucose and insulin levels are also scarce and insufficient.
EN
The effects of potato protein concentrates (PPC) differing in dietary solanidine glycoalkaloids (SGA) and trypsin inhibitor activity (TI), and of potato sprouts with differing SGA levels, were studied in rats. In Experiment 1, semi-purified isoprotein diets containing casein or one of six PPC contributing from 116 to 439 mg SGA · kg–1 and from 0.13 to 0.45 mg · g–1 of TI activity, were used. Weight gain was significantly greater on the diet containing PPC with low SGA and low TI than on PPC with high SGA and moderate TI contents, and also higher than on casein. Weight gain was also slightly depressed on diets with the highest TI activity. Substitution of PPC for casein resulted in lowering the pH of caecal digesta and increasing short-chain fatty acids concentration. Feeding diets with the greatest SGA content induced depression of protein and organic matter digestibility while diets with the greatest TI activity decreased protein digestibility but did not affect pancreas weight. In Experiment 2, a commercial rat diet, supplemented with increasing amounts of potato sprout meal providing SGA in the range from 0 to 300 mg · kg–1, was used. Neither growth performance, organ weights, caecal parameters, nor enzyme activities were affected by the diet. It is postulated that the less evident response of rats to SGA in the natural ingredient than in the semi-purified diet was due to the attenuating effect of more intensive bacterial fermentation. Potato trypsin inhibitor should be considered as an antinutritional factor in PPC; interactive effects of SGA and TI are presumable.
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