Wyniki wyszukiwania - Biblioteka Nauki

1

Separation of matrine and oxymatrine from Sophora flavescens extract through cation exchange resin coupled with macroporous absorption resin

100%

Chen H. , Luo S. , Zheng X. , Fan H.

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tom Vol. 18, nr 2

31--39

EN

A simple method for separation of matrine and oxymatrine from Sophora flavescens was developed with cation exchange resin coupled with macroporous resin. Based on the adsorption characteristics of matrine and oxymatrine, 001×732 cation exchange resin was used to absorb target alkaloids for removing most of the foreign matter, while BS-65 macroporous resin was chosen to purify these alkaloids. The result showed that the equilibrium adsorption data of matrine and oxymatrine on 001×732 resin and BS-65 resin at 30°C was fitted to Langmuir isotherm and Freundlich isotherm, respectively. The contents of matrine and oxymatrine were increased from 0.73% and 2.2% in the crude extract of the root of Sophora flavescens to 67.2% and 66.8% in the final eluent products with the recoveries of 90.3% and 86.9%, respectively.

2

Impact of various 2, 4-D concentrations and different vitamin mixtures on in vitro culture of coconut (Cocos nucifera L.) by utilizing seedlings shoot tip

100%

El-Gioushy S. F. , Rui L. , Fan H.

Acta Scientiarum Polonorum. Hortorum Cultus

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2020

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tom 19

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nr 2

EN

In this experiment, seedlings as sources for shoot tips was assembled from the coconut research institute (CRI) of Chinese Academy of tropical agricultural sciences germplasm, Wenchang, Hainan, China. The shoot tips were cultured on Y3 medium to study the impact of ten concentrations of 2, 4-D and three vitamin mixtures on survival, death and development % on in vitro culture establishment stage of coconut (Cocos nucifera L.). The obtained results revealed that all 2,4-D concentrations 4,7, 75, 100, 120 and 140 mg/L + any vitamin mixture did not give any development and high concentrations of 2, 4-D increased the death %. In any case, it can be concluded that the rate of differences in survival, death and development percentages exhibited by 2, 4-D concentrations was more pronounced than the analogous ones resulted by vitamin mixtures. At any rate, utilizing of (2, 4-D at concentration 25 mg/L+ vitamin mixture 1 or 2) exhibited the greatest values of survival percentage and reduced death percentage. Just as, increased the percentages of development in in vitro culture establishment stage of coconut (Cocos nucifera L.) through seedlings shoot tip.

3

Comparative study of PCR-based approaches for the genetic characterization of three strains of Acidithiobacillus caldus isolated from different sites in China

88%

Wu X. , Duan H. , Fan H. , Zhang Z. , Liu L.

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nr 4

EN

Comparative study of the genetic characteristics among three Acidithiobacillus caldus strains isolated from different typical environments in China was performed using a combination of molecular methods, namely sequencing analysis of PCR-amplified 16S rRNA genes and 16S-23S rRNA gene intergenic spacers (ITS), repetitive element PCR (rep-PCR), arbitrarily primed PCR (AP-PCR) fingerprinting and random amplified polymorphic DNA (RAPD). Both of the 16S rRNA gene and 16S-23S rRNA gene intergenic spacers sequences of the three strains exhibited small variations, with 99.9-100%, 99.7-100% identity respectively. In contrast, according to the analysis of bacterial diversity based on rep-PCR and AP-PCR fingerprinting, they produced highly discriminatory banding patterns, and the similarity values between them varied from 61.97% to 71.64%. RAPD analysis showed that banding profiles of their genomic DNA exhibited obvious differences from each other with 53.44-75% similarity. These results suggested that in contrast to 16S rRNA genes and 16S-23S rRNA gene intergenic spacers sequencing analysis, rep-PCR, AP-PCR fingerprinting and RAPD analysis possessed higher discriminatory power in identifying these closely related strains. And they could be used as rapid and highly discriminatory typing techniques in studying bacterial diversity, especially in differentiating bacteria within Acidithiobacillus caldus.

4

Numerical study of wave transmission over double submerged breakwaters using non-hydrostatic wave model

88%

Liang B. , Wu G. , Liu F. , Fan H. , Li H.

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tom No. 57 (4)

308--317

EN

In the present work, a non-hydrostatic wave model SWASH (an acronym of Simulating WAves till SHore) is used to simulate the wave transmission over double trapezoidal submerged breakwaters. The numerical results were compared with the results of the physical model. The comparison indicated the capability of SWASH model to predict the wave transmission over double submerged breakwaters. Influencing factors such as breakwater spacing S/L0, where L0 is the deep-water wavelength, and current were investigated in detail. Moreover, the effects of current on wave transmission were also analyzed. When the relative submerged depth R/H, where R is the submerged depth and H is the wave height, remains at 1.0, the appropriate relative breakwater spacing S/L0 is about 1.11. Current has no obvious effect on the appropriate S/L0, but it will change the shape of wave spectrum. Dissipation of super harmonic wave components is more obvious than that of lower harmonic wave components.

5

Investigation on biomass performance of a submerged membrane bioreactor for treating soy sauce wastewater

88%

Lv S. , Liang Z. , Li X. , Fan H. , Zen Y.

Environment Protection Engineering

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2016

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tom Vol. 42, nr 1

135--148

EN

A pilot study for investigating a membrane bioreactor (MBR) performance in treating soy sauce wastewater was divided into three stages. At stage 1, the MBR was operated without excess sludge withdrawal, and pollutants removal increased gradually and entered into a pseudo-stable phase eventually along with increasing mixed liquor suspended solid (MLSS). At stage 2, the growth of biomass and removal of pollutants were obviously decreased by lowering temperature. At stage 3, membrane permeate flux and sludge yield under various MLSSs were studied. Additional polishing steps including coagulation and oxidation were validated to be effective to ensure the treated wastewater to meet the discharge limits.

6

Numerical study of wave transmission over double submerged breakwaters using non-hydrostatic wave model

88%

Liang B. , Wu G. , Liu F. , Fan H. , Li H.

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tom 57

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nr 4

7

A comparative cell wall proteomic analysis of cucumber leaves under Sphaerotheca fuliginea stress

75%

Meng X. , Song T. , Fan H. , Yu Y. , Cui N. , Zhao J. , Meng K.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 11

8

Natural Fibres From the Bark of Mulberry Branches for Textile Application

75%

Dong Z. , Ding Z. , Zhang S. , Zhang Y. , Fan H. , Yang Y.

Fibres & Textiles in Eastern Europe

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2017

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tom Vol. 25, no. 3 (123)

20--25

EN

Fibers with low gum content were extracted from the bark of mulberry branches by a combination of bacteria and peroxide treatments. The bark of mulberry branches, with 30% cellulose, is a copious and inexpensive source of natural fibers. However, fibers extracted by microwave, enzyme or alkali had a high gum content (15.5% for hemicellulose and 8.6% for lignin), which rendered them difficult to be made into high- value textiles. In this research, strains with high polygalacturonase activities and subsequent hydrogen peroxide decreased the hemicellulose content to 2.5% and lignin content to 2.4%. Mulberry fibers in our study could be spun into yarns with a fineness of 18.2 tex. Compared to flax yarns, mulberry fiber yarns had a tenacity 20% higher, an elongation 18% higher and an unevenness 30% lower. Cotton/mulberry fiber fabrics had softer and smoother hand than cotton/flax fabrics. Overall, the fibers in our study show better potential for industrial textile applications than those in previous studies.

PL

Kora gałęzi morwy jest bogatym i niedrogim źródłem włókien naturalnych. W pracy przedstawiono proces otrzymywania włókien z kory morwy z wykorzystaniem szczepów bakteryjnych oraz nadtlenku wodoru, co zmniejszyło zawartość hemicelulozy do 2,5% i zawartości ligniny do 2,4%. W porównaniu do przędz lnianych, przędze z włókien morwy wykazywały o 20% wyższą wytrzymałość, o 18% wyższe wydłużenie i o 30% niższą nierównomierność. Tkaniny bawełniane z dodatkiem morwy miały bardziej miękki i gładszy chwyt, niż tkaniny bawełniane z dodatkiem lnu. Stwierdzono, że metoda pozyskiwania włókien z kory morwy przy zastosowaniu bakterii i nadtlenku wodoru może być stosowana do wyodrębniania włókien morwowych w celu produkcji wyrobów o wysokiej wartości i szerokim spektrum możliwości zastosowań przemysłowych.

9

Effects of iletamine-zolazepam-xylazine-tramadol combination on biochemical and haematological parameters in cats

75%

Li L. , Dong J. , Lu D. , Jiang S. , Lin D. , Fan H.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 3

EN

Cats were injected with 2.4 mg/kg of tiletamine-zolazepam, 0.36 mg/kg of xylazine, and 0.8 mg/kg of tramadol, then blood was collected from their peripheral veins at 0.5, 1, 4, 8, 24, 48, 72, and 168 h after the injection. Before injection, the same cats were used as control group (0 h). Biochemical and haematological parameters were measured. The results revealed that the combination of the compounds produced good immobilisation, with minimal changes over time in biochemical and haematological parameters, which were within biologically acceptable limits.

10

The accumulation of pigment in fiber related to proanthocyanidins synthesis for brown cotton

75%

Li T. , Fan H. , Li Z. , Wei J. , Lin Y. , Cai Y.

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nr 2

EN

Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.

11

The involvement of TsFtsH8 in Thellungiella salsuginea tolerance to cold and high light stresses

75%

Liu X. X. , Fu C. , Yang W. W. , Zhang Q. , Fan H. , Liu J.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 03

12

Short‑term exposure to high‑intensity sound induces hearing loss and apoptosis in guinea pigs

75%

Liu Y. , Wang H. , Liu Z. , Gu Y. , Xin L. , Liu J. , Fan H.

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2019

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tom 79

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nr 2

EN

The aim of this study was to investigate the effect and underlying mechanisms of short-term high-intensity sound exposure on guinea pigs to mimic the effects of non-lethal anti-riot weapons. A total of 92 male adult guinea pigs were exposed to high-intensity sound at 0 dB, 110 dB and 130 dB for 5 min. Basic clinical observation, repellent behaviour detection, peripheral blood routine examination, serum cortisol detection and hearing ability assessment were performed to analyse the functional changes after high intensity sound exposure. Meanwhile, routine haematoxylin and eosin staining, scanning electron microscopy and transmission electron microscopy were used to observe the structure of the cochlear tissue. To investigate the mechanisms underlying the tissue changes, the levels of apoptosis and caspase 3, 8 and 9 were detected using TUNEL staining and immunohistochemistry. After short-term exposure to high-intensity sound, the guinea pigs exhibited fear and agitation, increased repulsive behaviour, high serum cortisol and an increase in auditory threshold. The inner hair cells and outer hair cells exhibited degeneration. In addition, apoptosis was observed in the cochlear tissue. After short-term exposure to high-intensity sound, the guinea pigs exhibited not only stress reactions but also impaired hearing and signs of hair cell degeneration. Apoptosis in the cochlear tissue may play an important role in the functional and structural injuries caused by high-intensity sound.

13

Proteome-level investigation of Cucumis sativus - derived resistance to Sphaerotheca fuliginea

75%

Fan H. , Ren L. , Meng X. , Song T. , Meng K. , Yu Y.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 07

EN

Powdery mildew, caused by Sphaerotheca fuliginea (Sf), is a widely distributed and destructive disease of greenhouse and field-grown cucumber plants and causes great yield loss. The objective of this research is to tentatively identify proteins that are differentially expressed in cucumber and are involved in modulating resistance to Sf-inoculation. We comparatively analyzed proteins differentially expressed in Sf-inoculated cucumber leaves using a pair of sister lines, B21-a-2-2-2 (highly susceptible) and B21-a-2-1-2 (highly resistant). To eliminate the interference of ribulose-1,5-bisphosphate carboxylase with low-abundance proteins, total proteins were pre-fractionated by 24 % polyethylene glycol (PEG) and the proteins from supernatant were analyzed by 2-DE. We were successful in establishing the identities of 20 proteins and those identified from the resistant line included proteins involved in metabolic, regulatory, and defense pathways. Our findings are discussed within the context of C. sativus–S. fuliginea interaction and tolerance to this pathogen. The results suggest that the resistance in cucumber is closely related to the enhancement of its primary metabolism, and ethylene modulated signaling in cucumber defense responses against powdery mildew, then defense-related proteins can be up-regulated as a result of altered gene expression.

14

Salinity improves chilling resistance in Suaeda salsa

75%

Cheng S. , Yang Z. , Wang M. , Song J. , Sui N. , Fan H.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 07

EN

Suaeda salsa L., a C3 euhalophytic herb, is native to saline soils, demonstrates high resistance to salinity stress. The effect of chilling stress on S. salsa under high salinity, particularly the change in unsaturated fatty acid content within membrane lipids, has not been investigated. After a 12 h chilling treatment (4°C) performed under low irradiance (100 μmol m⁻² s⁻¹), the chlorophyll contents, maximal photochemical efficiency of photosystem II (Fv/Fm) and actual PSII efficiency (ΦPSII) were determined. These measurements were significantly decreased in S. salsa leaves in the absence of salt treatment yet there were no significant changes with a 200 mM NaCl treatment. Chlorophyll contents, Fv/Fm and ΦPSII in S. salsa under 200 mM NaCl were higher than those without salt treatment. The unsaturated fatty acid content and the double bond index (DBI) of major membrane lipids of monogalactosyldiacylglycerols, digalactosyldiacylglycerols (DGDG), sulphoquinovosyldiacylglycerols and phosphatidylglycerols (PG) significantly increased following the chilling treatment (4°C) (with 12 h of low irradiance and 200 mM of NaCl). The DBI of DGDG and PG was decreased in the absence of the salt treatment. These results suggest that in the euhalophyte S. salsa, a 200 mM NaCl treatment increases chilling tolerance under conditions of low irradiance (100 μmol m⁻² s⁻¹).

15

Analysis of heavy metal sources and health risk assessment of typical coal mine collapsed lakes in Huaibei Coalfield, Anhui Province, China

63%

Li J. , Gui H. , Hu R. , Chen L. , Xia Y. , Fan H. , Yu H. , Wang M.

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nr 5

16

Genome-wide detection of selective signatures in Simmental cattle

63%

Fan H. , Wu Y. , Qi X. , Zhang J. , Li J. , Gao X. , Zhang L. , Li J. , Gao H.

Journal of Applied Genetics

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2014

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tom 55

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nr 3

17

Hydrothermal synthesis and thermoelectric properties of PbS

63%

Zhang H. , Wang H. , Zhu H. , Li H. , Su T. , Li S. , Hu M. , Fan H.

Materials Science Poland

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2016

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tom Vol. 34, No. 4

754--759

EN

In this paper, hydrothermal approach combined with high pressure sintering method was employed to synthesize PbS. The X-ray diffraction results show that single phase PbS can be obtained by a simple hydrothermal method. The scanning electron microscope results show that the PbS sample has nearly cubic shape and preserves well crystallized and coarse grains after high pressure sintering. The thermoelectric performance of PbS obtained in this study is comparable to that of a PbS sample prepared by conventional method. The carrier type and concentration of PbS can be tuned effectively by doping with Bi. The maximum figure of merit for PbS doped with 1 mol% Bi reaches 0.44 at 550 K, which is about 30 % higher than that of undoped PbS. These results indicate that hydrothermal method provides a viable and controllable way of tuning the electrical transport and thermoelectric properties for PbS.

18

High temperature thermoelectric properties of PbTe prepared by high pressure method

63%

Fan H. , Su T. , Li H. , Zeng Y. , Li S. , Hu M. , Ma H. , Jia X.

Materials Science Poland

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2015

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tom Vol. 33, No. 1

152--156

EN

Thermoelectric materials PbTe were synthesized by high pressure method. The microstructure and temperature dependence of thermoelectric properties were studied. Some second phases with nano-structure were found in the high pressure synthesized samples. Compared with that of PbTe prepared at an atmospheric pressure, the samples prepared by high pressure method had smaller grain sizes, lower resistivity and lower phonon thermal conductivity. The maximum figure of merit, Z reached 8.86 × 104 K1 for the sample prepared at 4 GPa. This value was about twice times higher than that of the sample prepared at ambient pressure and it matched to that of highly doped PbTe.