Wyniki wyszukiwania - Biblioteka Nauki

1

The role of adhesion protein CD44 in the shape changes of astrocytes

100%

Konopka A. , Swiech L. , Jaworski J. , Wilczynski G. M. , Dzwonek J.

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nr Suppl.1

EN

CD44 is a widely distributed type I transmembrane glycoprotein and functions as the major hyaluronan receptor on most cell types. CD44 through interaction with actin cytoskeleton affects the transmission of signals from the outside to the inside of the cell in many tissues and organs. Primary cultures of astrocyte are diverse in their morphology and many factors can influence on it. In vivo astrocyte also are able to change their shape in response to various stimuli. The appearance of reactive astrocytes in vivo with thicker and longer processes and increased cellular content of glial fibrillary acidic protein (GFAP) has been observed in the CNS after various types of injury caused by physical, chemical, and pathological trauma. Furthermore, it has been showed that CD44 expression increases after brain injury. In our study we investigated the influence of knock down of CD44 by specific shRNA and CD44 overexpression on the astrocytes shape changes. Our results indicate that knock down of CD44 in astrocytes results in more regular and flat shape. In contrast the overexpression of CD44 promotes more irregular, radial-like shape of astrocyte. Our data support the hypothesis that CD44 plays role in morphological changes of astrocyte and give the opportunity to investigate its role in pathological processes such as brain injury.

2

Association between exposure to air pollution and incidence of atrial fibrillation

100%

Blaszczyk R. T. , Gorlo A. , Dukacz M. , Konopka A. , Glowniak A.

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2023

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tom 30

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nr 1

3

Wpływ jednorazowych i dwukrotnych odpasów w żywieniu loch karmiących na efekty odchowu prosiąt i kondycję loch w technologii fermy Bisprol

100%

Rak B. , Kapelanska J. , Kapelanski W. , Kujawiak J. , Konopka A.

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tom 264

4

Electrodes with various amount of nickel for truck frame repairs

100%

Miros M. , Majewski W. , Piwnik J. , Węgrzyn T. , Konopka A.

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tom T. 11, z. 4

21--27

EN

The goal of this paper is to analyze the exploitation and safety conditions of weld steel structure of car body truck frames after welding repairs made by covered electrodes. The main role of conditions in connected with materials, alloy elements in steel and filer materials, welding technology, state of stress and temperature. Because of that, good selection of steel and choice of welding method for proper steel structure repairs are very important. For responsible steel, structures like truck frames and low alloy steel are used, very often with small amounts of carbon and some amounts of alloy elements such as Ni and Mo in low alloy steel and their welds. To study the frame’s flexibility and resistance, welded joints have been analyzed. Samples have been welded by steel electrodes with various amounts of Ni.

PL

Celem artykułu jest analiza właściwości eksploatacyjnych oraz bezpieczeństwa naprawianych ram pojazdów ciężarowych z użyciem spawania. Ważną rolę odgrywają połączenia materiałów, pierwiastki stopowe, materiały dodatkowe, technologia spawania, stan naprężeń oraz temperatura. Bardzo istotne są odpowiedni dobór stali oraz metody spawalniczej. Na odpowiedzialne konstrukcje stalowe, jak np. ramy pojazdów ciężarowych, jest używana niskostopowa stal, często z niską zawartością węgla oraz dodatkiem pierwiastków stopowych, takich jak Ni oraz Mo. Do oceny elastyczności oraz wytrzymałości połączeń spawanych w ramach pojazdów ciężarowych, analizowano właściwości połączeń wykonanych elektrodami o różnej zawartości Ni.

5

Expression and function of extracellular matrix metalloproteinase-9 in human epilepsy

72%

Konopka A. , Grajkowska W. , Ziemianska K. , Roszkowski M. , Rysz A. , Koperski L. , Kaczmarek L. , Wilczynski G. , Dzwonek J.

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nr S

EN

Focal cortical dysplasia (FCD) is a developmental brain disorder characterized by abnormalities of cytoarchitecture and neuronal morphology. FCD is associated with pharmacologically intractable forms of epilepsy in both children and adults. The mechanisms that underlie FCD-associated seizures are unclear. It is believed that a pathological plasticity, including abnormality of synaptic connections, plays the crucial role in this disease. Recent studies indicate the role of interactions between nerve cells and the extracellular matrix in the processes of plasticity. Matrix metalloproteinases are enzymes, which are able to degrade the extracellular matrix components, so they can play an important role in these interactions. Results of experiments using rodent models showed that extracellular matrix metalloproteinase-9 (MMP-9) can play an important role in epileptogenesis. There is no data demonstrating that MMP-9 is involved in the development of epilepsy in human. The aim of this study was to determine whether MMP-9 might play a role in FCD - related epilepsy. Expression of MMP-9 was investigated in human brain tissue derived from people suffering from epilepsy. The immunohistochemistry and antibody microarray methods were used. The control group consisted of the autopsy brain samples. The results indicate that the expression of MMP-9 in the human brain tissue with FCD is increased. The highest immunoreactivity occurs in cytoplasm of abnormal neurons. Moreover, among the 7 tested matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13), MMP-9 is present in greatest concentration in the FCD tissue homogenates. The results support the hypothesis of the possible role of MMP-9 in the development of human epilepsy and give an opportunity to develop new treatments.

6

Quality of life of children suffering from motor disabilities as evaluated by their parents

72%

Wojtkowski J. , Sienkiewicz D. , Okurowska-Zawada B. , Paszko-Patej G. , Konopka A. , Okulczyk K. , Średzińska K. , Dmitruk E. , Mirska A. , Kułak W.

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tom 7(2)

60-66

EN

Purpose: We assessed the quality of life of children with motor disabilities in comparison with healthy children, as evaluated by their parents, using the CHQ-PF28 questionnaire (Child Health Questionnaire—Parent Form). Materials and methods: In a prospective study, we evaluated the quality of life of 105 children with motor disabilities. Results: Our research showed lower quality of life in the group of children with motor disabilities compared with controls, both in terms of physical and psychosocial health. Significant correlations between independent walking and physical functioning, general behavior, and mental health of children suffering from motor disabilities were found. According to the average indices of quality of life of children suffering from motor disabilities, depending on sex, the greatest differences occurred in behavior and change of health status, while the smallest differences in self-esteem and parental involvement, compared with controls. In the case of healthy children, the largest differences appeared in the perception of pain, behavior, and self-esteem; whereas, the smallest variations occurred in the change of health status and physical activity. Conclusion: Children suffering from motor disabilities demonstrate lower quality of life compared with healthy children.