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Evaluation of the virucidal activity of Iris flavissima Pall

100%

Rajtar B. , Machalska A. , Jakubiec A. , Polz-Dacewicz M. , Otgonbaatar U. , Dungerdorj D.

Bulletin of the Veterinary Institute in Puławy

2008

tom 52

nr 3

The aim of the study was the evaluation of the cytotoxicity and virucidal activity of the extracts from underground parts of Iris flavissima Pall. The plant material was extracted using: methanol/H₂O (1:1 v/v) and methanol. The examination of the cytotoxicity of the extracts in the concentration of 0.025; 0.05; 0.1; 0.25; 0.5; 1; 2, and 5 mg mL⁻¹ was carried out on GMK cell cultures. The cultures were incubated at 37°C in an atmosphere with 5% of CO₂ for 24-72 h. The cytotoxicity was measured by the colorimetric MTT (tetrazolium) method. Human enterovirus ECHO 9 was used for the evaluation of the virucidal activity of the extracts. The suspension of the virus was mixed (1:1 v/v) with the examined extracts at the concentrations non-toxic to GMK cell cultures and incubated at 37°C for 1 h, and then the virus was titrated in the GMK cell culture. The titre of the virus was estimated according to the Reed-Muench method. The methanolic and methanol/H₂O extracts were found to be non-toxic to GMK cells at the concentration of 1 mg ml⁻¹ and 2 mg ml⁻¹, respectively. The virucidal effect was observed for the both investigated extracts. The methanolic extract decreased ECHO 9 replication by 1.46 log, and methanol/H₂O extract - by 2.08 log.

Screening for phenolic acids in five species of Iris collected in Mongolia

84%

Machalska A. , Skalicka-Woźniak K. , Widelski J. , Głowniak K. , Purevsuren G. , Oyun Z. , Khishgee D. , Urjin B.

Acta Chromatographica

2008

tom Vol. 20, no. 2

259-267

Five species of Iris commonly used in Mongolian traditional medicine (Iris dichotoma Pall., Iris flavissima Pall., Iris bungei Maxim., Iris lactea Pall., and Iris tenuifolia Pall.) were analyzed for the presence of phenolic acids. This was the first study of the phenolic acid content of these species. Samples containing the phenolic acids were prepared by the method of Świątek and then analysed by HPLC with UV-visible diodearray detection (DAD). Identification was performed by comparing retention times with those of standards. Quantitative determination was performed at the absorbance maximum of each phenolic acid (320 nm for ferulic, p-coumaric, and caffeic acids, 280 nm for trans-cinnamic, syringic, and gallic acids, and 254 nm for vanillic, m-hydroxybenzoic, phydroxybenzoic, and protocatechuic acids). As the result of our study ten phenolic acids, both free and liberated by alkaline and acid hydrolysis, were identified by HPLC. Chromatographic investigation revealed the presence of vanillic acid, protocatechuic acid, trans-cinnamic acid, p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, gallic acid, syringic acid, m-hydroxybenzoic acid, and caffeic acid. Quantitative analysis of these acids was also performed. Finally, the presence or absence of some phenolic acids after alkaline or acid hydrolysis was also observed.