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1 Polish Journal of Environmental Studies

1 2017

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Redox mediators assisted-degradation of direct yellow 4

100%

Nouren S. , Bhatti H. N. , Iqbal M. , Bibi I. , Nazar N. , Iqbat D. N. , Kanwal Q. , Kausar A. , Hussain F.

tom 26

nr 6

Direct yellow 4 degradation was investigated in the presence of various mediators (p-coumaric acid, 1, hydroxybenzotriazole (HOBT), syringaldehyde, vanillin, syringic acid, veratryl alcohol, and pyrocatechol) at pre-optimized conditions of process variables, i.e., pH 5.0, temperature 50ºC, enzyme dose 24 U/mL, and 0.25 mM H₂O₂ concentration. Citrus limon peroxidase (CL-POD) was used for degradation of DY4 dye. In the absence of mediators, the DY4 degradation was 60%, whereas mediators enhanced the POD biodegradation efficiency up to 87%. Among mediators investigated, syringaldehyde showed promising efficiency. We investigated yringaldehyde concentrations in the range of 0.0125-0.5 mM, and 0.025 mM was optimum for maximum dye degradation, which revealed that mediators could be used to enhance the biodegradation of dyes.