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Wystepowanie beta-laktamaz typu ESBL u paleczek z rodzaju Serratia

100%

Ulatowska B. , Czajkowski H. , Gospodarek H. , Mikucka A.

Medycyna Doświadczalna i Mikrobiologia

2000

tom 52

nr 1

17-24

Enzymy o rozszerzonym spektrum działania (ESBL) wśród klinicznych szczepów pałeczek z rodzaju Serratia wykrywano metodą dwóch krążków (w niewielkiej modyfikacji). Badania wykazały, że 57,7% szczepów dawało reakcję dodatnią.

Serratia spp. has been identified as an important opportunistic pathogen agent in nosocomial infections. The aim of the study was the determination of extended spectrum ß-lactamases (ESBL) occurrence among 78 of Serratia spp. strains isolated in 1996-1998 from clinical specimens obtainted from patients of State Clinical Hospital in Bydgoszcz. Identification of Serratia spp. strains was performed in automatic ATB system with ID 32GN strips (bioMérieux). The strains with ESBL activity were detected by double-disc method according to Jarlier et al. (10) with small modifications. Clavulanic acid, tazobactam and sulbactam were used as the inhibitors of ESBLs. Drug-susceptibility was determinated by disc-diffusion method according to NCCLS standards. Forty-five (57,7%) of the strains were ESBL (+). All of them belonged to S. marcescens species. The majority - 91,1% of strains was derived from urine, 3 from wound and 1 from blood. The obtained results indicate the necessity of monitoring of ESBL-producing strains among gram-negative rods from clinical specimen. The aims of such a procedure are to control and to prevent their dissemination within hospital, as well as to avoid therapeutic failures.

Właściwości hemaglutynacyjne Escherichia coli

75%

Janicka G. , Bialek M. , Kruszynska E. , Harenska K. , Mikucka A. , Belzyt E. , Czajkowski H.

Diagnostyka Laboratoryjna

1998

tom 34

nr 2

Wrazliwosc na antybiotyki szczepow Staphylococcus aureus

63%

Kruszynska E. , Bialek M. , Janicka G. , Bugalski R. , Belzyt E. , Czajkowski H. , Harenska K. , Wojciechowska D.

Medycyna Doświadczalna i Mikrobiologia

1997

tom 49

nr 3-4

141-144

Staphylococcus aureus is a very important pathogen in humans about 20%of all bacterial infections are caused by S. aureus. Because the staphylococcal sensitivity patterns have changed, the aim of this study was to investigate the current susceptibility of the S. aureus strains to 9 antibiotics: penicilin (P), amoxacillin / clavulanic acid (AMC), erythromycin (E), cortimoxazole (SXT), tetracycline (T), chloramphenicol (C), mupirocin (MUP), gentamicin (Ge) and vancomycin (Va). Susceptibility testing was performed by disc diffusion technique, by the procedure outlined by the National Committe for Clinical Laboratory Standards (NCCLS). Susceptibility to methicillin was determined using 5 µm methicillin disks, ß-lactamase production in the penicillin - resistant strains was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology system). The microbiological characteristics of the samples: 338 of the isolates were derived, from noses and the throaths 382 - from surgical wound secretions. The analysis of the results showed that most S. aureus strains (82,8%) are penicillin resistant and ß-lactamase producing 13,5% of all strains were methicillin - resistant. 16,5% - were erythromycin resistant; 3,2% - were cotrimoxazolc resistant; 51,4% - were mupirocin resistant; 20,6% - were gentamicin resistant. All of the strains were vancomycin sensitive.