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EN
The aim of the present work was to evaluate the influence of exercise on serum homocysteine and oxidative stress in Thoroughbreds during official races. For our study, ten Thoroughbred mares, 5 years old and clinically healthy, were used. The horses were trained to take part in an official 2100 meter race. Reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. Blood samples, collected from each horse at rest, immediately after the race, and 30 and 180 min after the end of the race, were collected by jugular venipuncture using vacutainer tubes with no additive for the assessment of dROMs, Oxy-adsorbent and SHp by means of a UV spectrophotometer, while serum total Hey values were determined by high performance liquid chromatography with fluorimetric detection and isocratic elution. Statistical analysis, one way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P<0.05 was considered statistically significant) for all parameters studied. Moreover, a coefficient of linear correlation (r) was computed for values of dROMs and sHcy for all sampling times but a significant linear regression (r = 0.94) was found only after the race. The variations of sHcy, dROMs, Oxy-adsorbent and SHp during the experimental period considered suggest the important role of oxidative status in the athletic horse. The systematic analysis of oxidative stress and its influence on homocysteine levels contribute to the clinical evaluation and assessment of the athletic performance of the horse.
EN
The daily rhythm of platelet aggregation and clotting parameters during training were investigated in six Thoroughbred horses. Blood samples were collected every 4 h for 48 h. Platelet aggregation was measured with an aggregometer and subsequently according to the trends recorded, the percentage of aggregation was calculated. Prothrombin time (PT), activated partial thromboplastin time, thrombin time, and fibrinogen concentration were assessed by means of a coagulometer. A one-way repeated- measures analysis of variance was used to determine significant differences (P<0.05) and the single cosinor method was applied. On each single day, a significant effect of time on the percentage of aggregation and PT was observed. A cosinor analysis identified the nictemeral rhythm of percentage of aggregation and PT that showed a nocturnal acrophases on both days 1 and 2. We can assert that physical exercise could be an event regulating the daily rhythms of platelet aggregation and clotting parameters in the horse.
EN
The neonatal period represents a transition phase from the sheltered intrauterine to the exposed extrauterine environment. Changes in haematological profile in five pairs of twin kids and their dams were investigated over a month after the birth. During that period red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and platelets (PLT) level were determined in all animals. Two-way ANOVA showed an increase in RBC (P<0.001), WBC (P<0.0001), MCV (P<0.0001), MCHC (P<0.0001) and PLT (P<0.001). Knowledge of the blood physiological reference values in goat kids provides information useful for the diagnosis and treatment of some neonatal diseases and contributes to recognizing the adaptation mechanisms developing over their first month of life.
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