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Grafted serotonergic neurons can reverse changes in gene expression in motoneurons produced by spinal cord injury in rats

100%

Miazga K. , Joachimiak E. , Fabczak H. , Slawinska U.

Acta Neurobiologiae Experimentalis

2015

tom 75

nr Supl.

BACKGROUND AND AIMS: Serotonin, which is supplied to the spinal cord by serotoninergic cells localized in the raphe nuclei and parapiramidal areas of the medulla, plays a very important role in control of the spinal locomotor central pattern generator (CPG). In our previous study we showed that intraperitoneal application of: 8-OH-DPAT (5-HT1A and 5-HT7 serotonin receptor agonist) and quipazine (mainly 5-HT2A serotonin receptor agonist), or intraspinal transplantation of serotonergic cells isolated from 14-day old rat embryo brain stem, facilitates locomotor-like hindlimb movements in spinal rats (spinal cord total transection between Th9 and Th10). 5-HT7 and 5-HT2 serotonin receptor antagonists blocked the locomotor-like hindlimb movements that had been restored in spinal rats grafted with embryonic serotoninergic cells. The aim of the present study was to examine the influence of spinal cord total transection and transplantation of serotonin neurons isolated from the 14-day old rat embryo brain stem on changes in expression of genes encoding 5-HT2A, 5-HT2C and 5-HT7 serotonin receptors in populations of motoneurons innervating tibialis anterior, gastrocnemius lateralis, and extensor caudae medialis muscles. METHODS: For motoneurons labeling a method of retrograde staining using intra muscle injection with cholera toxin B subunit conjugated with Alexa Fluor 555 was used. Motoneurons were then collected by using the laser capture micro-dissection method, and changes in expression of genes encoding serotonin receptors were analyzed by Real-time PCR. RESULTS: The results show that total spinal cord transection changed expression of genes encoding 5-HT2A, 5-HT2C and 5-HT7 serotonin receptors in ankle flexor and ankle and tail extensor muscles. Grafting of serotonin neurons reverses the effects of spinal cord injury on expression of these genes. CONCLUSION: This is the first demonstration that grafts of serotonergic neurons can reverse changes in gene expression in motoneurons produced by spinal cord injury.

The expression of gamma-tabulin gene changes during the cell cycle, and its dependence on the microtubular cytoskeleton network in a ciliate Tetrahymena pyriformis

100%

Joachimiak E. , Miceli C. , Kaczanowska J.

Cellular and Molecular Biology Letters

2002

tom 07

nr Suppl.

Two antibodies against membrane skeleton proteins: epiplasmin B [135kDa] and fenestrin [64 kDa] are markers of the cell polarity of the ciliate Tetrahymena thermophila

100%

Kaczanowska J. , Joachimiak E. , Kiersnowska M. , Kaczanowski A.

Cellular and Molecular Biology Letters

2002

tom 07

nr Suppl.

Syndrome of the failure to turn off mitotic activity in Tetrahymena thermophila: in cdaA1 phenotypes

100%

Joachimiak E. , Kaczanowska J. , Kiersnowska M. , Kaczanowski A.

Acta Protozoologica

2004

tom 43

nr 4

Rola cytoszkieletu w rozwoju organizmow jednokomorkowych [na przykladzie orzeska Tetrahymena i porownawczo drozdza Schisosaccharomyces pombe]

88%

Kaczanowska J. , Wloga D. , Kiersnowska M. , Joachimiak E. , Kaczanowski A.

Kosmos

2000

tom 49

nr 4

537-557

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

75%

Kotwica J. , Ciuk M. A. , Joachimiak E. , Rowinski S. , Cymborowski B. , Bebas P.

Journal of Physiology and Pharmacology. Supplement

2006

tom 57

nr 8

107-123

The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.