In this paper several studies were carried out using a sterilization method with a corona electrical discharge plasma. A tip-to-plane configuration were used with negative and positive polarity power supplies to perform a parametric sterilization studies on E. coli (ATCC 25922) and Bacillus subtilis (ATCC168) bacteria. Plasma has several effects, but the most important are the bacteria destruction on packaging surface and food products for safer products and less likely to be contaminated during longer storage, and the non-aggressive sterilization of heat-sensitive surgical instruments such as endoscopes. These studies present the bacteria resistance and interaction phenomenon by each polarity of the direct current (DC) plasma source on the surfaces. The obtained results show that relatively short treatment time of the order of few minutes is sufficient to remove a significant wet charge of E. coli (Gram G-) by positive polarity and only 11 min by negative polarity. On the other hand, only 65% of Bacillus subtilis (Gram G+) charge was sterilized by positive polarity and 12% by negative polarity for 40 min treatment time. To conclude this work, a comparison was made between the two polarities.
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W artykule przedstawiono szereg metod sterylizacji z wykorzystaniem plazmy przy wyładowaniu koronowym. Badano efekt przy różnych rodzajach bakterii. Stwierdzono pełną sterylizację po czasie od kilku do kilkunastu minut. Ale w przypadku niektórych bakterii nawet 40 minut dawało efekt tylko w 65%.
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