Wyniki wyszukiwania - Biblioteka Nauki

1

Grzyby patogenne jako źródło insektycydów

100%

Bogus M. I.

Biotechnologia

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2000

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nr 3

2

Drapiezne grzyby nicieniobojcze

63%

Czygier M. , Bogus M. I.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 1

25-31

EN

Gastrointestinal nematodes are considered a serious economic problem affecting the livestock industry around the world. Current methods of their control, relaying mainly on organic drugs, are not sustainable because parasites develop resistance to anthelmintic and bacause of increasing public concem about chemicals residues in livestock products and environment. Nematode-trapping fungi offer a very promissing, nonchemotherapeutic approach to nematode parasite control. Their potential in preventing nematodosis is well documented. In this paper we outline the present knowlege on mechanisms involved in trapping and killing nematodes by the predacious nematode-destroying fungi.

3

Enzymatic activities analysis of Conidiobolus coronatus colonics with high and low pathogenicity to Galleria mellonella larvae

63%

Wieloch W. , Bogus M. I.

Acta Biochimica Polonica

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2006

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tom 53

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nr Supplement 1

4

The effect of fungal infection on the hemolymph composition of Neurotoma nemoralis larvae

63%

Bogus M. I. , Wloka E.

Pestycydy

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2005

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nr 3

177-181

5

Optimization of reaction conditions to determine mycelial elastolytic activity of the entomopathogenic fungus Conidiobolus coronatus

63%

Wloka E. , Bogus M. I.

Acta Biochimica Polonica

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2006

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tom 53

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nr Supplement 1

6

The influence of hibernation on Dendrolimus pini haemocytes

63%

Kedra E. , Bogus M. I.

Pestycydy

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2005

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nr 4

139-142

7

Exploring pathogenicity potential of Conidiobolus coronatus against insect larvae in various infection conditions

63%

Wieloch W. , Bogus M. I.

Pestycydy

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2005

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nr 4

133-137

8

Extraction of an insecticidal protein fraction from the parasitic fungus Conidiobolus coronatus [Entomophthorales]

63%

Bogus M. I. , Scheller K.

Acta Parasitologica

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2002

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tom 47

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nr 1

EN

From our experiments it appears that parasitic fungus, Conidiobolus coronatus, infected 12 insect species we have tested, while another 8 species did not suffer after contact with this pathogen. The culture filtrates and mycelial homogenates of C. coronatus contain toxic proteins. By means of two steps FPLC: gel filtration followed by ion-exchange chromatography, we isolated a 30 kDa toxic protein from the culture filtrate. The 30 kDa protein appeared to be very potent on Galleria mellonella larvae: LD₅₀ was less than 5 ng/larva. Rabbit polyclonal anti-30 kDa immunoglobulins specifically recognized this fungal toxin. Using antibodies we showed that 30 kDa mycotoxin is present in C. coronatus conidia, hyphae and is released by the fungus into the incubation liquid medium.

9

Histopathology of Conidiobolus coronatus [Entomophthorales] infection in Galleria mellonella [Lepidoptera] larvae

63%

Bogus M. I. , Szczepanik M.

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nr 1

EN

The histopathology of Conidiobolus coronatus infected Galleria mellonella larvae was investigated. In order to distinguish between morphological changes accompanying the dying process and changes connected with the activation of host-defense mechanisms, two fungal strains were used: A(+) killing 90-100% of Galleria larvae and A(-) causing 20-35% mortality in infected hosts. C. coronatus infection resulted in the appearance of black spots on the insect cuticle. Cross-sections revealed callosity and melanization of the cuticle at the infection site. Melanized epidermal cells together with the haemocytes accumulating beneath the place of infection formed a haemocytic capsule. Characteristic changes were observed in the Malpighian tubule morphology of insects surviving infection. Up to 60% of tubules inside survivor become brown, thick and fragile. Histological studies of the deformed tubules revealed the disappearance of the brush border and pycnosis of epithelial cell nuclei taking place at the time of termination of the exposure to the fungal pathogen. Twenty four hours later lumens of deformed Malpighian tubules were filled to capacity with a brown substance and surrounded by numerous layers of haemocytes forming a non-melanized nodule. All other organs of the infected Galleria larvae remained unchanged. No fungal structures were found inside the haemocoel and organs of the host larvae. The fat bodies of the survivors as well as of the dying insects were not changed and contained lipid droplets, suggesting that larval death was due to mycotoxin(s) produced by the pathogenic fungus Saprophytic development of C. coronatus was observed in the case of only one cadaver out of the 53 investigated.

10

Encapsulation potential of insect haemocytes towards entomopathogenic fungus Conidiobolus coronatus

63%

Kedra E. , Bogus M. I.

Pestycydy

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2004

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nr 3-4

11

The role of enzymes produced by entomopathogenic fungi in pathogenesis of insects

63%

Czygier M. , Bogus M. I.

Pestycydy

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2000

|

nr 1-2

12

Grzyby entomopatogenne jako potencjalne zrodlo insektycydow

51%

Bogus M. I. , Modlinska E. , Samborski J.

Pestycydy

|

1997

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nr 1-2

39-51

13

Wplyw wolnych kwasow tluszczowych na wzrost, sporulacje i wirulencje pasozytniczego grzyba Candidiobolus coronatus

38%

Babiarz E. , Bogus M. I. , Czygier M. , Kucinska J. , Samborski J. , Szafranek J.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 4

763-768

EN

Five free fatty acids (FFA): C16:0, C18:0, C18:1, C18:2 and C18:3 were introduced into culture media in order to investigate differential development of pathogenic fungus Conidiobolus coronatus as a function of FFA concentration. All tested FFA showed fungistatic action inhibiting hyphae growth and sporulation. Fungal colonies grown in the presence of FFA showed decreased virulence.