Changes in selected FAs level as influenced by inclusion of fresh lucerne (Medicago sativa L.) into a feeding ration were assesed in Czech Fleckvieh (dual purpose) and Holstein cows. Each of the balanced groups of 12 cows in mid-lactation was fed with a ration containing 12.7 kg (11.7% of dry matter) of fresh lucerne and then with a ration with preserved forage (maize and grass silages) only. Total content of nutritionally desirable C18 acids was 34.1 and 30.5% of total fatty acids in Czech Fleckvieh and Holstein cows, respectively (P=0.0693). The response of the breeds on the change in feeding differed. The changes in fatty acid composition in the Czech Fleckvieh cows were less extensive in stearic and oleic acids, while more extensive in essential linoleic and linolenic acids than those in the Holstein cows. Addition of lucerne into ration in both breeds has caused greatest changes in polyunsaturated fatty acids (P<0.01), particularly alpha-linolenic acid (P<0.001).
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