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3 Polish Journal of Environmental Studies

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Mitochondrial permeability to ions induced by dibenzodioxines and dibenzofuranes. A simple method to measure ion permeability in membranes

100%

Bragadin M. , Perin G. , Manente S. , Raccanelli S.

Polish Journal of Environmental Studies

1997

tom 06

nr 5

This paper proposes a method to demonstrate that 123789 Dibenzodioxine and 2378 Dibenzofurane enhance membrane permeability to the ions in mitochondria from rat liver. The procedure can be employed: i) as a general method to assess the enhancement of membrane permeability by toxic compounds, ii) to study the decrease in conductivity of carrier-systems induced by inhibitors of transport, and iii) to study the behaviour of ion-carrier systems which are potentially dependent on biological membranes.

The respiratory chain of rat liver mitochondria as a probe for toxicological tests in water

100%

Bragadin M. , Manente S. , Jero A. , Perin G.

tom 07

nr 3

The respiratory rate of the mitochondrial respiratory chain is very sensitive to many toxins; some toxins enhance, while others slow the respiratory rate. We propose, therefore, a new simple "in vitro" method in order to assess acute toxicity in water using the response of the mitochondrial respiratory chain from rat liver. On the basis of the response we subdivided all tested toxins into two categories and for each toxin the EC50I value (which is the dose of toxin necessary to halve the maximal respiratory rate) and the EC50u value (which is the uncoupler dose necessary to stimulate the respiratory rate up to fifty percent of its maximum value) are calculated. The comparison between EC50 and LC50 values obtained by fish method allowed us to conclude that our method is a good tool for predicting the toxicity response in fish.

A study of the toxic effects of six dibenzofuranes in mitochondria from rat liver

75%

Manente S. , Iero A. , Fabris R. , Perin G. , Rizzoli V. , Bragadin M.

Polish Journal of Environmental Studies

1999

tom 08

nr 3