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PL
W listopadzie 2009 roku w potoku Rzepnik, w dopływie Skawinki (dorzecze Górnej Wisły), odłowiono 5 kiełbi białopłetwych Romanogobio albipinnatus complex. Ryby te preferują duże nizinne rzeki, dlatego nowe stanowisko w zaledwie 10-kilometrowym cieku o szerokości koryta wynoszącej 2–3 m jest bardzo nietypowe. Jest to pierwsze stwierdzenie kiełbia białopłetwego na terenie Małopolski
EN
Five specimens of Romanogobio albipinnatus complex (Fig. 1) were caught in the Rzepnik Stream (49°59´24˝N, 19°48´20˝E; a tributary of the Skawinka River, within the Upper Vistula drainage basin). The gudgeons were of 52.0–57.5 mm in standard length. It is a very unusual occurrence site of R. albipinnatus (Fig. 2), which commonly inhabits large lowland rivers. Due to general morphological resemblance to G. gobio complex, small size and mainly nocturnal activity, the knowledge about the distribution of the whitefin gudgeon in the territory of Poland is very limited and it seems underestimated
EN
While the ichthyofauna of Polish rivers is relatively well documented, few data are available on the ichthyofauna in running streams on fish farms. The present study focused on three streams at the Experimental Fisheries Station of the University of Agriculture in Krakow. During ten catches conducted from March 2011 to March 2012, a total of 626 fish belonging to 16 species were caught. Stone moroko, Pseudorasbora parva, which is an invasive alien species, was also noted. A brown trout spawning nest was observed in one of the streams. This indicates the important role of such ecosystems in preserving biodiversity.
EN
Background. Endocrine disrupting chemicals (EDCs), among them polychlorinated biphenyls (PCBs), are able to change the hormonal regulation of reproduction at the hypothalamo-pituitary-gonadal (HPG) axis in many vertebrates. The aim of the presently reported study was to determine the effects of highly chlorinated PCBs mixture—Delor 106—on the in vivo and in vitro luteinizing hormone (LH) secretion from the pituitary gland of female Prussian carp, Carassius gibelio (Bloch, 1782), at the time of natural spawning. Materials and Methods. In the in vivo experiment we tested exposure to Delor 106: acute (one week at the concentration of 700 ng · L–1) and chronic (5 weeks at increasing weekly concentrations, from 140 through 700 ng · L–1). Blood samples from all females were taken before intoxication (0 week) and subsequently every week (week 1 through 5) till the end of the experiment. In the in vitro experiment enzymatically dispersed pituitary cells obtained from sexually mature Prussian carp females were incubated for 5 or 24 h in the presence of Delor 106 at the concentrations of: 1, 5, 10, 50, or 100 ng · mL–1 of medium. LH levels in the incubation medium as well as in blood plasma were measured using ELISA method. Results. Long-term (3 and 4 weeks) exposure to the Delor 106, gradually added to the water, caused a significant elevation of spontaneous LH secretion. Pituitary cells incubation with Delor 106 at the concentration range from 10 through 100 ng · mL–1 of medium resulted in the significant increase of spontaneous LH secretion after 5 and 24 h. Conclusion. The results obtained in this study show that highly chlorinated biphenyl—Delor 106—changes the secretion of luteinizing hormone in Prussian carp in the spawning season, acting, at least in part, directly at the pituitary level.
EN
The effect of different cadmium concentrations on sperm motility in common carp was investigated. The motile activity of spermatozoa was evaluated by means of computer assisted sperm analysis (CASA) using three major parameters characterizing sperm movement - VCL, VAP and VSL. Moreover, subjective microscopic observations were performed in order to evaluate the average time of sperm movement. The following cadmium concentrations were tested: 10,50 100, 200, 500, 1.000 and 2.000 ppm. Computer assisted analysis and microscopic observations both showed that cadmium decreases the motility of carp spermatozoa in all tested concentrations, and that lethal effects were detectable at a concentration of 500 ppm (as determined by CASA) or 1.000 ppm (when manual microscopic observations were performed). Additionally, it was shown that low cadmium concentrations have the most negative influence on straight line velocity, which suggests the possible negative influence of cadmium on the ability of sperm to fertilize female gametes.
PL
W niniejszej pracy badano wplyw różnych stężeń kadmu na ruchliwość plemników karpia. Aktywność ruchową plemników oceniano za pomocą wspomaganej komputerowo analizy plemników (CASA) z wykorzystaniem trzech parametrów charakteryzujących poruszanie się plemników - VCL, VAP i VSL. W ramach niniejszych badań mierzono także czas ruchliwości plemników metodą obserwacji mikroskopowych. W doświadczeniach zastosowano następujące stężenia kadmu: 10,50 100, 200, 500, 1000 oraz 2000 ppm. Zarówno komputerowa analiza ruchliwości, jak i obserwacje mikroskopowe wykazaly, że kadm hamuje ruchliwość plemników wykazując efekt letalny przy koncentracji 500 ppm (w przypadku metody komputerowej CASA - rys. 1) lub 1000 ppm (w przypadku subiektywnej oceny czasu trwania ruchu plemników - rys. 2). Wykazano ponadto, że niskie stężenia kadmu w największym stopniu obniżają ruchliwość plemników mierzoną parametrem VSL, co sugeruje prawdopodobny negatywny wplyw tego metalu na zdolność plemników do zaplemnienia.
EN
The aim of the study was to determine the diversity of fish fauna and zoobenthos in three pond canals of the Experimental Fisheries Station of the University of Agriculture in Krakow. The physicochemical parameters of the water were also measured. A total of 1,056 fish of 23 species was caught during four sampling catches performed from May 2013 to August 2013. Six of the species identified were aliens. The number of zoobenthos families noted was similar and ranged from eight to 15 depending on the canal and month. The results suggest that the ecological role of the pond canals is significant.
EN
Background. Polychlorinated biphenyls (PCBs) are persistent and bioacumulative chemical pollutants which exert negative physiological effects on the reproductive system of mature male and female fish. PCBs present in the aquatic environment may also have an influence on developing embryos. The aim of this study was to investigate the effect of Aroclor 1254, a polychlorinated biphenyls mixture, on the embryonic development of Prussian carp, Carassius gibelio (Bloch, 1782), and common carp, Cyprinus carpio L. Materials and Methods. The samples of eggs obtained from 4 females of each species were divided into two dishes and incubated separately after fertilization (common carp) or activation (Prussian carp) with common carp sperm. The incubation, with Aroclor 1254 (1 or 10 ng · mL–1) or in water with no PCB added, lasted for 4 days. The mortality, hatching rate, number of hatched larvae, and number of deformed larvae were observed. Results. After 24 h of incubation of Prussian carp and common carp eggs there were no significant differences in the percentage of living eggs between the Aroclor 1254 treated groups and the control one. The lowest tested concentration of PCB (1 ng · mL–1) accelerated the hatching of Prussian carp larvae at 75 h of incubation. The significant increase in the percentage of deformed larvae was observed only in the experiment with common carp eggs incubated with Aroclor 1254 at the concentration of 10 ng · mL–1. Conclusion. Results of the presented data showed that Aroclor 1254 (at tested concentrations) is not harmful for the development of activated Prussian carp eggs but teratogenic effect was observed in the case of common carp embryos.
EN
The aim of this study was to determine the growth rates of pikeperch, Sander lucioperca (L.), and pike, Esox lucius L., from the Tresna Reservoir. Back-calculations were used to determine fish age. Histological pictures were used to determine the spawning success of the two species compared. The study material was comprised of 18 pikeperch females and 13 males, and 20 pike females and 12 males. The growth rate of pikeperch from the Tresna Reservoir was higher in comparison to populations inhabiting other basins in Poland. The histological analyses of the gonads indicated that pikeperch spawned successfully in the reservoir. The growth rate of the pike in the reservoir was comparable to that of this species in other basins. However, the histological picture of the ovaries indicated that spawning was only partially successful since 25% of females did not spawn and their eggs were in a state of resorption.
PL
Celem badań było określenie tempa wzrostu sandacza, Sander lucioperca (L.) i szczupaka, Esox lucius L. ze zbiornika zaporowego Tresna. Wiek ryb określono metodą odczytów wstecznych. Na podstawie analizy histologicznej gonad określono sukces tarłowy porównywanych gatunków. Materiał badawczy stanowiło 18 samic i 13 samców sandacza oraz 20 samic i 12 samców szczupaka (tab. 1). Tempo wzrostu sandacza w zbiorniku Tresna było wyższe w porównaniu z populacjami żyjącymi w innych akwenach w Polsce (tab. 2). Analiza histologiczna gonad wskazuje na sukces tarłowy sandacza w zbiorniku. Tempo wzrostu szczupaka w zbiorniku Tresna jest porównywalne z tempem wzrostu tych ryb w innych akwenach (tab. 3), natomiast obraz histologiczny jajników wskazywał na częściowy sukces tarłowy, gdyż 25% samic nie przystąpiło do rozrodu i ikra znajdowała się w stanie resorpcji.
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