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2 Journal of Applied Genetics

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DNA damage in mouse lymphocytes exposed to curcumin and copper

100%

Urbina-Cano P. , Bobadilla-Morales L. , Ramirez-Herrera M. A. , Corona-Rivera J. R. , Mendoza-Magana M. L. , Troyo-Sanroman R. , Corona-Rivera A.

nr 4

Dietary polyphenolics, such as curcumin, have shown antioxidant and anti-inflammatory effects. Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper. The aim of this study was to evaluate the in vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay. Balb-C mouse lymphocytes were exposed to 50 µM curcumin and various concentrations of copper (10 µM, 100 µM and 200 µM). Cellular DNA damage was detected by means of the alkaline comet assay. Our results show that 50 µM curcumin in the presence of 100-200 µM copper induced DNA damage in murine lymphocytes. Curcumin did not inhibit the oxidative DNA damage caused by 50 µM H₂O₂ in mouse lymphocytes. Moreover, 50 µM curcumin alone was capable of inducing DNA strand breaks under the tested conditions. The increased DNA damage by 50 µM curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay.

Protective in vitro effect of curcumin on copper genotoxicity evaluated by comet and micronucleus assays

84%

Corona-Rivera A. , Urbina-Cano P. , Bobadilla-Morales L. , de J. V.-L. J. , Ramirez-Herrera M. A. , Mendoza-Magana M. L. , Troyo-Sanroman R. , Diaz-Esquivel P. , Corona-Rivera J. R.

2007

tom 48

nr 4

389-396

Curcumin is a phytochemical with antiinflammatory, antioxidant and anticarcinogenic activities. Apparently, curcumin is not genotoxic in vivo, but in vitro copper and curcumin interactions induce genetic damage. The aim of this study was to test if in vivo copper excess induces DNA damage measured by comet and micronucleus assays in the presence of curcumin. We tested 0.2% curcumin in Balb-C mice at normal (13 ppm) and high (65,130 and 390 ppm) copper ion concentrations. The comet and micronucleus assays were performed 48 hr after chemical application. Comet tail length in animals treated with 0.2% curcumin was not significantly different from the control. Animals exposed to copper cations (up to 390 ppm) exhibited higher oxidative DNA damage. Curcumin reduced the DNA damage induced by 390 ppm copper. We observed statistically significant increase in damage in individuals exposed to 390 ppm copper versus the control or curcumin groups, which was lowered by the presence of curcumin. Qualitative data on comets evidenced that cells from individuals exposed to 390 ppm copper had longer tails (categories 3 and 4) than in 390 ppm copper + curcumin. A statistically significant increase in frequency of micronucleated erythrocytes (MNE/10000TE) was observed only in 390 ppm copper versus the control and curcumin alone. Also cytotoxicity measured as the frequency of polychromatic erythrocytes (PE/1000TE) was attributable to 390 ppm copper. The lowest cytotoxic effect observed was attributed to curcumin. In vivo exposure to 0.2% curcumin for 48 hr did not cause genomic damage, while 390 ppm copper was genotoxic, but DNA damage induced by 390 ppm copper was diminished by curcumin. Curcumin seems to exert a genoprotective effect against DNA damage induced by high concentrations of copper cations. The comet and micronucleus assays prove to be suitable tools to detect DNA damage by copper in the presence of curcumin.