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EN
The purpose of the research carried out in the Kampinos Forest in 1963 was to determine the toxoplasm prevalence among the birds. The smears of the brain, pancreas, liver and blood were tested for the presence of the Protozoa. The preparations on the slides were stained with the Giemza method. A total of 330 birds belonging to 27 species were examined. No toxoplasm was found in 1550 preparations. However, the studies revealed the presence of the Protozoa of the family Haemoproteidae (Doflein, 1916) belonging to the Haemoproteus and Leucocytozoon genera. The Haemoproteus fringillae was found in the blood of 11 bird species, and Leucocytozoon sp. in the blood of 2 species.
EN
An investigation for toxoplasmosis was carried out with 50 women with pregnant pathology (the delivery of foetuses with developing defectiveness, spontanous abortion and mortification of pregnancy), as well as with 50 healthy women making a controlling group. In the former group positive serological and allergic reactions for ioxoplasmosis were much more frequent than in the latter. Combination. mother Rh+ father Rh - occured twice more frequently in the group with the pregnant pathology than in the controlling one. Blood analysis of the mothers with positive reactions for toxoplasmosis showed slight devi.ations in the form of somewhat increased lymphocytosis and monocytosis and sometimes somewhat increased acid-absorbing leucocyte number. Passage carried out in 23 cases on the mice chosen of suspected material and the search of parasite under the microscope did not bring positive results. The whole of investigations did not lead the formerly appointed aim, i. e. to the definition of the role of toxoplasmosis in the pregnant pathology because of the imperfection of diagnostic methods. They make however grounds to the conclusion that toxoplasmosis in probably quite frequent disease here and in our present conditions a whole set of factors must decide about its diagnosis. These factors are leaded by a characteristic clinical picture and positive serological and allergic reactions for toxoplasmosis.
EN
A homogenate prepared from dried T. spiralis larvae was fractionated by means of paper electrophoresis on a starch block in borate buffer at pH of 8.6. The immunologie activity of fractions was examined by diffusion and gel inmmunoelectrophoresis using complement fixation, ring precipitation tests, as well as determinining protein, saccharides and enzyme levels. It was found that the fractions obtained possess the properties of protein antigens. Among 26 fractions, those from 9 to 14 proved to be serologically active. The antigens were found to contain acide phosphatase and hvaluronidases.
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