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1 Genetica Polonica

1 1994

1 Chwilkowska B.

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New effective method of obtaining chlorate-resistant cell lines of monohaploid potato

100%

Chwilkowska B.

nr 4

303-314

The studies were performed with the aim to develop a more effective method for obtaining in vitro chlorate-resistant potato cell lines with genetic changes of enzymes participating on mineral assimilation and used for studying metabolism of this pathway.These lines were isolated from Solanum tuberosum monohaploid cells (2n=x=12), plated into selection media, differing in KClO3 concentration and incubated in the suspension.Duplication of cell population in the suspension occured at day 7 of culture.A results of performed cell selection were 13 chlorate resistant cell lines, which may be referred to the so-called "stable variants".