Over a four-year period some microbiological aspects of soil subsystem and proportion of legumes were studied in permanent, oversown and temporary grassland under different level of fertilizer application. The research was carried out in Banská Bystrica, Slovakia, at the altitude of 460 m. The proportion of legumes markedly increased in temporary and oversown grasslands, but decreased with the fertilizer application. There was a positive relationship between CO₂ production in soil and the legume proportion in sward (Figures 1 and 3). However, the correlation between soil CO₂ production and the ratio of soil CO₂ with added N and glucose (NG) to basal CO₂ (B) production (NG/B) was negative. The ratio of NG/B is an indicator of soil organic matter quality (Figures 4 and 5). It was concluded that soil organic matter was more easily degradable in grassland with higher proportion of legumes.
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