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1

Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU)

100%

Pyziel A. M.

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tom 59

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nr 1

EN

Dictyocaulosis (Nematoda: Trichostrongyloidea) is a widespread parasitosis of the European bison (Bison bonasus) inhabiting Bialowieza Primeval Forest. Bearing in mind the current coexistence of bison with wild cervids, and with domestic ruminants in the 19th and 20th century, the need arose for molecular identification of lungworm species. Molecular analysis was done on adult lungworms that were obtained from the respiratory track of four free-roaming bison euthanized as a part of the population health control program. As the result of the study four identical small subunit-ribosomal RNA gene sequences from the lungworms were obtained and deposited in GenBank as sequence, 1708 bp long (GenBank KC771250). Comparative analysis of the SSU rRNA sequences revealed the European bison to be a host for the bovine lungworm Dictyocaulus viviparus.

2

Wystepowanie kokcydiow z rodzaju Eimeria u zubrow w Puszczy Bialowieskiej

63%

Demiaszkiewicz A. W. , Pyziel A. M.

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nr 1

27-30

3

Coprology of a single Northern white-breasted hedgehog (Erinaceus roumanicus): first report of Isospora rastegaievae in Poland

63%

Pyziel A. M. , Jezewski W.

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nr 3

4

Przypadek dirofilariozy powiekowej u psa w Polsce

63%

Demiaszkiewicz A. W. , Pyziel A. M.

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nr 04

5

Rola łosi (Alces alces) w rozprzestrzenianiu parazytoz

51%

Filip K. J. , Demiaszkiewicz A. W. , Pyziel A. M.

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nr 05

6

The first report of Angiostrongylus vasorum (Nematoda; Metastrongyloidea) in Poland, in red foxes (Vulpes vulpes)

51%

Demiaszkiewicz A. W. , Pyziel A. M. , Kuligowska I. , Lachowicz J.

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tom 59

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nr 4

EN

Angiostrongylus vasorum belongs to the superfamily of Metastrongyloidea. This nematode occurs in foxes, dogs and other predators. The Nematode A. vasorum place themselves in the pulmonary artery and its branches, and in the right ventricle and atrium of the heart. Numerous species of land snails are the intermediate hosts of the parasite. In 2013, lungs and hearts of 76 foxes shot in the Forest District Głęboki Bród in Augustowska Primeval Forest were parasitologically necropsied. Four of the examined foxes were infected with the nematode A. vasorum, a prevalence of 5.2%. In one fox pericardium there were 6 male and 6 female nematodes. In the remaining three foxes nematodes were localized in the pulmonary artery. In two foxes 2 specimens of nematodes were detected (male and female, and two females) while 1 female was detected in the other fox. This is the first report of the presence of the nematode A.vasorum in fox in Poland.

7

Analysis of 18S rRNA, ITS2 and cox1 sequences of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) from the European bison, roe deer and red deer in Poland: molecular evidence for a new species

51%

Pyziel A. M. , Laskowski Z. , Demiaszkiewicz A. W. , Hoglund J.

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tom 62

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nr Suppl.

8

Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from South-Western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2)

51%

Pyziel A. M. , Demiaszkiewicz A. W. , Kuligowska I.

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9

The first report of Aelurostrongylus falciformis (Schlegel, 1933) (Nematoda, Metastrongyloidea) in badger (Meles meles) in Poland

51%

Demiaszkiewicz A. W. , Filip K. J. , Pyziel A. M.

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nr 2

EN

Aelurostrongylus falciformis belongs to the superfamily of Metastrongyloidea. This nematode occurs in European badgers and locates in lungs, in respiratory tract. Numerous species of land snails are intermediate hosts of the parasite. In 2015, parasitological necropsy of 9 badgers, shot in the Forest District Głęboki Bród in Augustowska Primeval Forest, was performed. Two examined animals were infected with nematodes A. falciformis. In the lungs of each badger two specimens of nematodes were detected (male and female). In the following article, description, morphometrical data and figures are presented. This is the first report of A. falciformis infection in badgers in Poland.

10

Monitoring parazytologiczny żubrów w rezerwatach hodowlanych Białowieskiego Parku Narodowego

51%

Krzysiak M. K. , Demiaszkiewicz A. W. , Pyziel A. M. , Larska M.

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2015

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tom 71

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nr 12

EN

Parasite invasions are one of the main diagnosed pathologies of European bison. Since the severity and parasite species composition varies depending on the season, parasitological monitoring and deworming of European bison and evaluation of its effectiveness is important in the breeding of European bison. For that reason, parasitological evaluation based on coproscopical methods of live animals was performed. Full parasitological examinations of the digestive system together with necropsies were performed post-mortally. The obtained data enabled the selection of a suitable deworming treatment. The results showed that the parasite species composition in the fenced European bison breeding center was similar to the situation observed in free ranging European bison. The invasion of a blood sucking nematode Ashworthius sidemi was confirmed in European bison closed breeding for the first time. None of the European bison showed any clinical symptoms caused by parasitic factors. The research proved that from the clinical point of view the deworming process is more crucial in the spring, because of increased intensity and extensiveness of invasions during this time and the possibility for the use of chemotherapeutics with a broader spectrum of treatment.

11

Fascioloides magna pasożytem sarny w Borach Zielonogórskich

51%

Demiaszkiewicz A. W. , Kowalczyk R. , Filip K. J. , Pyziel A. M.

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nr 04

EN

The aim of this study was to determine the prevalence of infection with F. magna flukes in roe deer from the conifer forest Bory Zielonogórskie. Parasitological dissection was performed on 20 roe deer hunted in May 2016. Faecal samples from the animals were examined by the decantation method. One roebuck was verified to be infected with F. magna, which yielded a prevalence of 5%. We noted fibrinous deposits on the diaphragmatic surface of the liver with adhesions to the diaphragm, as well as five dark-coloured nodular protrusions on the liver surface. In the cross section view of the infected liver, we saw five pseudocysts corresponding to the nodular protrusions observed on the liver surface. Three of the pseudocysts were filled with dark brown liquid containing a few flukes, their eggs and cell detritus. The other two pseudocysts contained dark fluid and fragments of dead flukes. Although, no liver fluke eggs were detected in the faeces, thousands of eggs were found in the pseudocysts. Previous studies in Poland revealed two foci of this parasitosis: in south-western and south-eastern parts of the country. It is possible that the range of both foci is expanding due to red deer migrations and the spread of infected water snails and larval forms of the parasite in watercourses and rivers. Fascioloides magna infection is a threat for cervids and domestic ruminants. Therefore, it is important to monitor the occurrence of this fluke in Poland. It is essential to determine the range of fascioloidosis, examine the intermediate host species and record cases of F. magna infection in domestic ruminants.

12

Occurrence of tapeworms Moniezia benedeni (Moniez, 1879) in European bison Bison bonasus L. in Białowieża Primeval Forest

51%

Demiaszkiewicz A. W. , Pyziel A. M. , Lachowicz J. , Filip-Hutsch K.

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nr 1

13

Skuteczność odrobaczania żubrów utrzymywanych w Rezerwatach Hodowlanych Białowieskiego Parku Narodowego

45%

Krzysiak M. K. , Demiaszkiewicz A. W. , Lachowicz J. , Pyziel A. M. , Kuligowska I.

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nr 01

14

Nematodes of the large intestine of the European bison of the Bialowieza National Park

45%

Demiaszkiewicz A. W. , Pyziel A. M. , Kuligowska I. , Lachowicz J. , Krzysiak M. K.

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tom 58

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nr 1

EN

The present study was conducted on 31 males and females of the European bison, eliminated during the winter seasons 2007–2011 in the Białowieża Primeval Forest, Poland. The caeca of 14 free-ranging bison, aged from 3 months up to 16 years, the most favorable infection site for the large intestine nematodes, were investigated during the winter of 2007/2008. The parasitological autopsies of the large intestines of 9 free-ranging bison aged from 5 months up to 10 years as well as 9 bison kept in the close reserves aged from one up to 20 years were done during the winter seasons of 2008–2011 to determine localization of nematodes in large intestine and the total intensity of parasite infection. Five species of nematodes (i.e., Trichuris ovis, Oesophagostomum venulosum, O. radiatum, Ashworthius sidemi, Nematodirus helvetianus) were found in dissected caecum of bison culled during the winter of 2007/2008. During the seasons of 2008–2010, 6 species of nematodes were found in the large intestine of bison (i.e., T. ovis, O. venulosum, O. radiatum, A. sidemi, N. roscidus and Bunostomum trigonocephalum). We did not find any nematodes in the lumen of the large intestine of captive bison culled during the seasons of 2010/2011. The results of the present study indicate that the intensity of infection by the large intestine nematodes of the European bison in the Białowieża Primeval Forest has stayed at the comparable level throughout the last 20 years; however the number of nematode species has increased. The observed level of parasitic infection is typical of subclinical parasitoses.

15

Parasitological monitoring of European bison (Bison bonasus) in the Bieszczady Mountains

45%

Demiaszkiewicz A. W. , Pyziel A. M. , Filip-Hutsch K. , Januszczak M. , Olech W.

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nr 02

EN

The aim of our study was to determine the level of parasitic infections in European bison (Bison bonasus) in the Bieszczady Mountains on the basis of coprological methods: flotation, decantation and the Baermann technique. 429 faecal samples were collected from European bison in the following Forest Districts: Baligród, Komańcza, Lutowiska, Stuposiany, Lesko and Cisna. Examination of faeces revealed the presence of eggs of gastrointestinal nematodes from the Trichostrongylidae family, as well as Nematodirus sp., Aonchotheca sp. and Trichuris sp., larvae of lung nematodes Dictyocaulus viviparus, eggs of tapeworms Moniezia sp. and oocysts of 7 species of coccidia, belonging to the genus Eimeria (E. bovis, E. zuernii, E. pellita, E. bukidnonensis, E. subspherica, E. ellipsoidalis and E. brasiliensis). The population of European bison in the Bieszczady Mountains turned out to be the only free-living herd not infected with the liver fluke Fasciola hepatica. The level of parasitic infections, observed during present study, was low and did not indicate clinical signs of parasitic disease, thus it did not require deworming.

16

The expansion of nematodes Ashworthius sidemi in wild ruminants in Poland

45%

Demiaszkiewicz A. W. , Kuligowska I. , Lachowicz J. , Pyziel A. M. , Moskwa B.

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2013

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tom 59

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nr Suppl.

17

Pierwsze ogniska dirofilariozy psow wywolanej przez Dirofilaria repens Railliet et Henry, 1911 w centralnej Polsce

45%

Demiaszkiewicz A. W. , Polanczyk G. , Pyziel A. M. , Kuligowska I. , Lachowicz J.

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nr 4

367-370

18

The prevalence and distribution of Dirofilaria repens in dogs in the Mazovian Province of Central-Eastern Poland

38%

Demiaszkiewicz A. W. , Polanczyk G. , Osinska B. , Pyziel A. M. , Kuligowska I. , Lachowicz J. , Sikorski A.

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nr 4

19

Prevalence and distribution of Dirofilaria repens Railliet et Henry, 1911 in dogs in Poland

38%

Demiaszkiewicz A. W. , Polanczyk G. , Osinska B. , Pyziel A. M. , Kuligowska I. , Lachowicz J. , Sikorski A.

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nr 3

EN

In 2011-2013 1588 samples of dogs’ blood were examined for dirofilariosis using Knott method, as well as the Kingston and Morton method. The species of microfilariae was determined on the basis of morphometric characteristics. Samples were also examined using the Canine Heartworm Antigen Test. Positive samples were examined using a multiplex PCR assay for species confirmation. Microfilariae belonging to the species D. repens were found in the blood samples of dogs from all the provinces of Poland. The mean prevalence of this species observed in Poland was 11.7%. The range of intensity of infection was counted using the number of microfilariae found in 60 μl of blood amounted to between 1 and 158, and the mean intensity was 18 microfilariae. Microfilariae and antigens of D. immitis were not found in any examined blood samples.

20

Prevalence of Dirofilaria repens in dogs in central-eastern Poland and histopathological changes caused by this infection

38%

Osinska B. , Demiaszkiewicz A. W. , Pyziel A. M. , Kuligowska I. , Lachowicz J. , Dolka I.

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tom 58

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nr 1

EN

The aim of the study was to determine the prevalence of canine dirofilariosis in four provinces of central-eastern Poland and to describe pathological changes occurring during the course of this parasitosis. A total of 188 dogs aged from one and a half up to 16 years were examined for dirofilariosis. Blood samples were analysed for the presence of microfilariae using Knott method, as well as the method of Kingston and Morton. Pathomorphological examination of two dogs was also performed. Histological sections were stained with H&E, AB-PAS, van Gieson, and von Kossa methods. Microfilariae of Dirofilaria repens were found in blood samples of dogs that originated from the Mazowieckie, Lubelskie, and Podlaskie provinces. The mean prevalence of that species was 12.7%. Autopsy revealed the adult nematodes in the subcutaneous connective tissue. Microscopic examinations indicated the presence of microfilariae in the liver, kidneys, lungs, heart muscle, and intestines. Necrotic foci, thrombosis, and infiltrations of different intensity with eosinophils in internal organs were also observed. Significant lesions in different internal organs together with the presence of numerous microfilariae suggest their important role in the disease process.