Wyniki wyszukiwania - Biblioteka Nauki

1

Reconstituted mononucleosome as a model to study proteins binding to DNA damaged by cis-platinum

100%

Kalinowska M. , Pietrowska M. , Widlak P.

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nr Supplement 1

2

Detection and characterization of rat proteins recognizing and binding DNA damaged by N-acetoxyacetylaminofluorene and cis-platinum

100%

Pietrowska M. , Lanuszewska J. , Rzeszowska-Wolny J. , Widlak P.

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tom 05

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nr 2

3

Polimorphism of XPD gene coding for nucleotide excision repair protein and the risk of head and neck cancers

88%

Polanska J. , Jaworska J. , Pietrowska M. , Butkiewicz D. , Rzeszowska-Wolny J.

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nr Supplement 1

4

The detection and characterization of rat protein recognizing DNA damaged by N-acetoxy-acetylaminofluorene

88%

Pietrowska M. , Lanuszewska J. , Walter Z. , Rzeszowska-Wolny J. , Widlak P.

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tom 05

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nr 4

423-431

EN

Proteins recognizing DNA damaged by the chemical carcinogen N- acetoxy-acetylaminofluorene (AAAF) were analyzed in nuclear extracts from rat tissues, using a 36 bp oligonucleotide as the substrate and an electrophoretic mobility shift assay. Two major proteins that form complexes with DNA damaged by AAAF were detected; one of them also bound DNA damaged by cis-diammine-dichloroplatinum. The complex specific for AAAF-damaged DNA contained protein loosely attached to nuclear components. It was extracted with 0.1 M NaCl. The amount of this protein was estimated at about 105 copies per liver cell nucleus, and its probable size was about 42 kDa as detected by the Southwestern blotting assay. Its affinity for DNA damaged by AAAF was ~10-fold higher than that for undamaged DNA. Analogous AAAF- DDB (damaged-DNA-binding) proteins were also detected in extracts from rat brain, testis and kidney tissue. The levels of such proteins were not affected in rats treated with the carcinogen 2-acetylaminofluorene.

5

Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene

88%

Pietrowska M. , Rusin M. , Widlak P. , Razin S. V. , Rzeszowska-Wolny J.

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nr 3

EN

We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken α-globin gene domain (the fragment was designed as α-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic π-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb α-0 fragment decreased the activity of CAT reporter gene when transfected into chicken erythroblastoid cells.

6

TNFalpha-induced activation of NFkappaB protects against UV-induced apoptosis specifically in p53-proficient cells

75%

Szoltysek K. , Pietranek K. , Kalinowska-Herok M. , Pietrowska M. , Kimmel M. , Widlak P.

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2008

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tom 55

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nr 4

741-748

EN

The signaling pathways that depend on p53 or NFκB transcription factors are essential components of cellular responses to stress. In general, p53 is involved in either activation of cell cycle arrest or induction of apoptosis, while NFκB exerts mostly anti-apoptotic functions; both regulatory pathways apparently interfere with each other. Here we aimed to analyze the effects of NFκB activation on DNA damage-induced apoptosis, either p53-dependent or p53-independent, in a set of human cell lines. Four cell lines, HCT116 and RKO colon carcinoma, NCI-H1299 lung carcinoma and HL60 myeloblastoma, each of them in two congenic variants either containing or lacking transcriptionally competent p53, were used. Cells were incubated with TNFα cytokine to activate NFκB and then treated with ultraviolet or ionizing radiation to induce apoptosis, which was assessed by measurement of the sub-G1 cell fraction. We observed that treatment with TNFα resulted in a significant reduction in the frequency of apoptotic cells in UV-irradiated p53-proficient lines (with exception of the UV-resistant NCI-H1299 cells). This anti-apoptotic effect was lost when cells were pretreated with parthenolide, an inhibitor of NFκB activation. In marked contrast, TNFα-pretreatment of p53-deficient lines resulted in an increased frequency of apoptotic cells after UV irradiation (with exception of HL60 cells). Such anti- and pro-apoptotic influence of TNFα was less obvious in cells treated with ionizing radiation. The data clearly indicates functional interference of both signaling pathways upon the damage-induced apoptotic response, yet the observed effects are both cell type- and stimulus-specific.

7

Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma

63%

Jelonek K. , Wojakowska A. , Marczak L. , Muer A. , Tinhofer-Keilholz I. , Lysek-Gladysinska M. , Widlak P. , Pietrowska M.

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nr 2

8

Identification of serum proteome components associated with progression of non - small cell lung cancer

51%

Pietrowska M. , Jelonek K. , Michalak M. , Ros M. , Rodziewicz P. , Chmielewska K. , Polanski K. , Polanska J. , Godowicz-Klosok A. , Giglok M. , Suwinski R. , Tarnowski R. , Dziadziuszko R. , Rzyman W. , Widlak P.

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nr 2

EN

The aim of the present study was to perform comparative analysis of serum from patients with different stages of non-small cell lung cancer (NSCLC) using the three complementary proteomic approaches to identify proteome components associated with the progression of cancer. Serum samples were collected before any treatment from 200 patients with NSCLC, including 103 early stage, 64 locally advanced and 33 metastatic cancer samples, and from 200 donors without malignancy. The low-molecular-weight fraction of serum proteome was MALDI-profiled in all samples. Serum proteins were characterized using 2D-PAGE and LC-MS/MS approaches in a representative group of 30 donors. Several significant differences were detected between serum samples collected from patients with early stage cancer and patients with locally advanced cancer, as well as between patients with metastatic cancer and patients with local disease. Of note, serum components discriminating samples from early stage cancer and healthy persons were also detected. In general, about 70 differentiating serum proteins were identified, including inflammatory and acute phase proteins already reported to be associated with the progression of lung cancer (serum amyloid A or haptoglobin). Several differentiating proteins, including apolipoprotein H or apolipoprotein A1, were not previously associated with NSCLC. No significant differences in patterns of serum proteome components were detected between patients with adenocarcinoma and squamous cell carcinoma. In conclusion, we identified the biomarker candidates with potential importance for molecular proteomic staging of NSCLC. Additionally, several serum proteome components revealed their potential applicability in early detection of the lung cancer.