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EN
The genotypes worked out by the Wrocław taxonomy method were obtained by means of elimination of the environment influences from values of useful traits of winter wheat phenotypes. The drawn dendrites showed a wide differentiatio of genotypes of the Grana, Kaukaz and Maris Huntsman varieties. Highly fertile strains estimated in the tests of 1985 were bred. Thus it can be concluded about high breeding value of varieties on the basis of measurements of useful traits by the Wrocław taxonomy method.
RU
Из качеств хозяйственно-ценных признаков фенотипов сортов озимой пшеницы было исключено влияние среды и таким образом полученные генотипы были разработаны методом вроцлавской таксономии. Вычерченные дендриты проявляли большую дифференциацию генотипов сортов Грана, Кавказ и Марис Хунтсман. Из гибридов этих сортов были отобраны высокоурожайные роды, которые оценивались в опытах в 1985 г. Следовательно, можно говорить о высоких селекционных качествах сортов, на основании разработанных измерений хозяйственно-ценных признаков методом вроцлавской таксономии.
EN
Ion channels selective for chloride ions are present in all biological membranes, where they regulate the cell volume or membrane potential. Various chloride channels from mitochondrial membranes have been described in recent years. The aim of our study was to characterize the effect of stilbene derivatives on single-chloride channel activity in the inner mitochondrial membrane. The measurements were performed after the reconstitution into a planar lipid bilayer of the inner mitochondrial membranes from rat skeletal muscle (SMM), rat brain (BM) and heart (HM) mitochondria. After incorporation in a symmetric 450/450 mM KCl solution (cis/trans), the chloride channels were recorded with a mean conductance of 155 ± 5 pS (rat skeletal muscle) and 120 ± 16 pS (rat brain). The conductances of the chloride channels from the rat heart mitochondria in 250/50 mM KCl (cis/trans) gradient solutions were within the 70–130 pS range. The chloride channels were inhibited by these two stilbene derivatives: 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene-2,2′-disulfonic acid (SITS). The skeletal muscle mitochondrial chloride channel was blocked after the addition of 1 mM DIDS or SITS, whereas the brain mitochondrial channel was blocked by 300 μM DIDS or SITS. The chloride channel from the rat heart mitochondria was inhibited by 50–100 μM DIDS. The inhibitory effect of DIDS was irreversible. Our results confirm the presence of chloride channels sensitive to stilbene derivatives in the inner mitochondrial membrane from rat skeletal muscle, brain and heart cells.
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