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Polyfructans and fructooligosaccharides (FOS) ? occurrence, production and application

100%

Kubik C. , Piasecka K. , Anyszka A. , Bielecki S.

Biotechnologia

2006

nr 2

103-116

Information on beneficial impact of probiotic microorganisms on human health and effects of prebiotic oligosaccharides on proliferation of the latter microflora are presented in the first part of the article. Its further part focuses on fructans, i.e. structures of natural polyfructans and fructooligosaccharides, mechanisms of function of enzymes involved in their synthesis and methods of manufacturing of prebiotic fructooligosaccharides (FOS) in commercial scale.

Dextransucrase of Leuconostoc mesenteroides - mechanism of dextran and oligosaccharides biosynthesis

100%

Kubik C. , Sikora B. , Galas E.

Biotechnologia

2000

nr 2

98-110

Dextransucrase catalyses the synthesis of a-D-glucopyranosyl homopolymers and oligomers from sucrose as a substrate. This paper is a review of reports on the mechanism of dextran and oligosaccharides synthesis. The presence of a low molecular acceptor dextran (3-20 kDa) or low molecular acceptor sugars in reaction mixtures enables obtaining of dextran with molecular weight in a range from 30 to 80 kDa or oligosaccharides with low d.p., which have gained increased nutritional interest (prebiotics). In these reactions, the hydrolytic enzyme, i.e. dextranase, may be involved together with dextransucrase instead of acceptors.