In computational biology the development of algorithms for the identification of tandem repeats in DNA sequences is a challenging problem. Tandem repeats identification is helpful in gene annotation, forensics, and the study of human evolution. In this work a signal processing algorithm based on adaptive S-transform, with Kaiser window, has been proposed for the exact and approximate tandem repeats detection. Usage of Kaiser window helped in identifying short as well as long tandem repeats. Thus, the limitation of earlier S-transform based algorithm that identified only microsatellites has been alleviated by this more versatile algorithm. The superiority of this algorithm has been established by comparative simulation studies with other reported methods.
In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1- O -β-D -galacturonide-4′-(1- O -β-D -glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1 , from the seeds of Cuminum cyminum . Compound 1 was separated isocratically on a C 18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques ( 1 H and 13 C NMR). The purity of 1 was also confirmed by HPTLC.
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