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XVII Miedzynarodowy Kongres International Association of Sexual Plant Reproduction Research [IASPRR]: Plciowe rozmnazanie roslin w naturze i laboratorium [Lublin, 9-13 lipca 2002]

100%

Strubinska J.

nr 3-4

67-71

Localization of vegetative nucleus and generative cell nuclei in branching pollen tubes of Oenothera hookeri L. grown in vitro

63%

Strubinska J. , Sniezko R.

nr 2

Oenothera hookeri L. pollen germination and pollen tube growth proceed in very similar ways in the pistil and in vitro. A characteristic feature of pollen grain germination is the emergence of one or more pollen tubes from 1, 2 or 3 colpi at the same time. The second specific event is the different way pollen tubes branch during their growth. The present study compares germination and pollen tube growth in fresh and frozen pollen grains. Pollen grain viability was estimated by fluorescein diacetate staining and tested by germination on medium. The localization of nuclear and plastid DNA was detected after DAPI staining. Sperm cell and vegetative nucleus positions were observed in only one of the pollen tube branches and pollen tubes germinating from the same pollen grain. The vegetative nucleus was weakly stained and its fluorescence was dim. The nuclei of sperm cells were very well seen and were found at different distances from the pollen tube tip. After a period of incubation the pollen tube containing sperm cells was always longer than other tubes or branches germinating from the same pollen grain. In some tubes the sperm cell nuclei seemed to change position from one branch to another. Sperm cells probably can move within the pollen tube growing in the ovary to the branch closest to the embryo sac.

Localization of nuclei in branching pollen tubes of Oenothera hookeri L. growing in vitro

63%

Sniezko R. , Strubinska J.

nr 1

The influence of lead and selenium interactions on shoot growth and antioxidant enzyme activity in Vicia faba L. minor plants

51%

Mroczek-Zdyrska M. , Strubinska J. , Hanaka A.

nr 3

Microscopic and spectroscopic analyses of selected agricultural formulations containing various nanostructures

51%

Kachel-Jakubowska M. , Strubinska J. , Matwijczuk A. , Gagos M.

nr 4

The aim of the present study was to analyse commercially available compounds containing nanoparticles in the form of Ag, Cu, and Ag sulphate colloids applied in agriculture. The compounds were analysed with TEM and FTIR spectroscopy, i.e., methods that can complement the commonly used research techniques such as EDX or Raman spectroscopy. The results of the microscopic examinations evidenced the presence of single spherical agglomerates (small granules) of the nanoparticle-containing products. Infrared spectroscopy revealed the presence of bands characteristic of vibrations that can be assigned to nanoparticle-X interactions, where X = S, C, or O in aqueous colloid solutions of the analysed samples. Additionally, bands characteristic for C-H stretching vibrations (probably associated with vibrations of the -CH₂ and -CH₃ groups) were observed as bands originating from compounds used as the carriers of the analysed systems. In the case of Ag sulphate, the most distinct band with a maximum at 2,863 cm⁻¹, which is characteristic of SH···Ag vibrations, can be observed, and its intensity may be associated with the nanostructure composition of the formulation, which is more diverse than that in the other two compounds.

Lead induced disturbances in cell divisions and growth in sunflower primary and adventitious roots

51%

Strubinska J. , Pietrusiewicz J. , Sniezko R. , Bednara J.

nr 2 Spec.Vol.