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Kamica szczawianowo-wapniowa u psów i kotów

100%

Wylupek D. , Madany J.

tom 87

nr 04

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

70%

Banach T. , Adaszek L. , Wylupek D. , Winiarczyk M. , Winiarczyk S.

nr 3

Proteomics including the studies of the structure, function and dependences between proteins is more and more extensively applied in human medicine and veterinary medicine. The analysis of protein profiles of tissues and body fluid from healthy and ill individuals allows to identify diagnostic, prognostic and predictive markers in various pathological states in people and animals. This paper presents preparation of urine samples for analysis in the mass spectrometer MALDI-TOF (Ultraflextreme, Bruker, Bremen, Germany) by means of two methods: liquid chromatography based on the system Nano-LC (PROTEINER FC II, Bruker Daltonics, Bremen Germany). and two-direction electrophoresis 2DE (GE Healthcare, United Kingdom). Both methods enable separation of the mixture under consideration into individual fractions of high purity indispensable for obtaining readable mass spectra. The purpose of this paper is to determine applicability of these methods in analysis of protein composition of urine samples.

Application of rhinoscopy in the diagnostics of nasal tumors in dogs

51%

Adaszek L. , Staniec M. , Buczek K. , Lopuszynski W. , Smiech A. , Milczak A. , Debiak P. , Klimiuk P. , Wylupek D. , Winiarczyk S.

tom 70

nr 01

The aim of this article was to describe cases of nasal tumors in dogs in which a rhinoscopy procedure was used as part of the process of disease diagnosis. The study included two dogs, aged 8 and 11 years, showing symptoms of epistaxis. The animals underwent a radiological examination and a rhinoscopy, during which bioptats were taken for histopathological examination. The radiological examination of the head did not reveal lesions characteristic of a neoplastic process. The rhinoscopic examinations showed a large hyperplasia closing the nasal canal in both dogs. The histopathological examination of the two bioptats sampled from the nose area demonstrated clusters of cells characteristic of a neoplastic process. The dogs were euthanized and subjected to a post-mortem examination. The histopathological examination of samples taken from the lesions in the nasal cavity confirmed olfactory neuroblastoma and transitional cell carcinoma in dogs 1 and 2, respectively. Rhinoscopy is a technique complementary to computer tomography, and, if the latter is impossible, it should represent, along with a radiological examination, the basis for a preliminary diagnosis of a neoplastic process, which ought to be confirmed by a cytological or histopathological examination of bioptats obtained from the sites of the lesions.