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EN
A new generation of photosensitizers for photodynamic therapy (PDT) has been investigated. Different amino acids were bound to protoporphyrin (PP). Two methods of determination the quantum yield of generated singlet oxygen in porphyrin solutions were investigated. In the first method, tryptophan was used as a singlet oxygen acceptor. In the second one, the amount of singlet oxygen was determined by phosphorescence at 1272 nm. The purpose of this work was to evaluate the quantum yield of singlet oxygen of different PP(AA)2Arg2 derivatives, since it is crucial for choosing proper ingredients of photosensitizer for diagnosis and therapy in PDT
EN
An new generation of photosensitizers for photodynamic therapy (PDT) has been investigated. Different amino acids (Ala, Ser, Thr, Phe and Trp) were bound to protoporphyrin (PP). The quantum yield of fluorescence of porphyrin solutions was investigated. Fluorescence spectra and lifetimes of excited porphyrins were determined, too. The purpose of this work was to evaluate the quantum yield of fluorescence of different PP(AA)2Arg2 derivatives, since it is crucial for choosing proper ingredients of photosensitizer for diagnosis and therapy in PDT
EN
Digital volume correlation is an image-based technique for internal 3D displacement and strain fields measurement or analysis widely used in the field of experimental mechanics. A widely used correlation function (criterion) of digital volume correlation is Pearson correlation function, which suffers from the problem of the acquired data being contaminated by salt-and-pepper noise and monotonic nonlinear distortion of the light intensity. In this work, a 3D correlation function called the Spearman correlation function is used to deal with those interferences. A numerical experiment shows that the performance of Spearman correlation function using integer-pixel registration in an environment with 10% salt-and-pepper noise is better than that of Spearman and Pearson correlation functions using sub-pixel registration in an environment with 1% salt-and-pepper noise. As the light intensity distortion is significant, the error of Pearson correlation function is considerable; meanwhile, the error of Spearman correlation function is small. In conclusion, Spearman correlation function is, in particular, practical and useful in digital volume correlation.
EN
The mitogen-activated protein kinase kinases (MKK) represent a small gene family that is located at the center of the MAPK cascade and play an important role in responses to biotic and abiotic stresses and in plant growth and development. Here, we report the cloning of an MKK gene from Brassica napus, BnMKK1 (GenBank Accession No. HQ916282), by RT-PCR. The BnMKK1 cDNA is 1,447 bp in length with an open reading frame of 1,092 bp. The gene encodes a putative MKK protein that contains a conserved motif S/TxxxxxS/T (where x represents any amino acid) and a MPK docking domain in its N-terminal extension. The orthologues of the BnMKK1 protein are highly conserved among mosses, ferns, dicotyledons and monocotyledons. Southern hybridization revealed the presence of more than two copies of the BnMKK1 homologues in the genome of B. napus. Quantitative reverse transcription–polymerase chain reaction analyses showed that the BnMKK1 transcripts accumulated in response to cold, ABA (abscisic acid) and MeJA (methyl jasmonate) but declined in response to mannitol, NaCl, H₂O₂, and SA (salicylic acid). The inhibitors of MAPK activation, PD98059 and U0126, did not inhibit BnMKK1 transcription. BnMKK1 transgenic tobacco plants grew slower and showed significantly delayed flower times compared to the wild type. Their root development was insensitive to treatment with 100 mM IAA (indole-3-acetic acid). The detached leaves from the transgenic BnMKK1 tobacco plants strengthened the inhibition to bacterial development at later growth stages. The overexpression of BnMKK1 leads to rapid water loss and enhanced sensitivities to drought stress in transgenic tobacco plants. These results show that BnMKK1 plays an important role in the response of plants to pathogenic bacteria and drought stress.
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