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EN
Recent reports indicate that mini chromosomes and other small genetic enti­ties may occur in the nuclei of uninfected higher plants. They become especially abundant under some special growth conditions and, sometimes, resemble extrachromosomal genes of ciliated protozoa. An example of such a gene-sized DNA species was isolated from resting wheat embryos. Hie presence of telo- meric sequences at its termini and the ability to replicate autonomously in wheat nuclei made it possible to distinguish this nuclear mini chromosome from chromosomal DNA fragmentation products. The biological significance of plant mini chromosomes remains to be elucidated.
EN
A fragment of wheat nuclear DNA was shown to be able to replicate autono­mously in wheat nuclei. The fragment was 637-bp long and contained telomeric repeats at both termini. Its replication was manifested by the appearance of a radioactive reaction product of the same approximate size. Further analyses showed that the linear, double-stranded réaction product was labelled along its entire length and contained the same number of similarly located HaelR sites as did the fragment tested. Prokaryotic DNA remained twlabelled under the same assay conditions.
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