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Czynniki wplywajace na proces biosyntezy N-glikanow w glikoproteinach

100%

Przybylo M.

nr 1

95-103

Age-related profile of beta-N-acetylhexosaminidase glycosylation in rat liver

72%

Litynska A. , Przybylo M.

tom 45

nr 3

β-N-acetylhexosaminidase was prepared from a liver lysosomal fraction obtained from rats between 18 days of gestation (group I) and 72 weeks of age (groups II-VI). A glycan chain analysis was performed after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lectins. The presence of high-mannose /hybrid type glycans, as well as their fucosylated forms was shown in all the experimental groups. Complex-type glycans with terminal sialic acid or galactose were present in all the groups except for 1-week-old rats in which only a positive reaction with lectins from Galanthus nivalis and Aleuria aurantia - was observed. Thus it may be assumed that age-related changes in the glycosylation pattern occur on the first days after birth.

Effect of pelleted cereal-based feed used in the diet on feed intake, eating behaviour, rumination and nutrient digestibility in antelope sitatunga (Tragelaphus spekii)

58%

Gorka P. , Przybylo M. , Kanski J. , Kloska A.

nr 2

The aim of the study was to determine the effect of pelleted cereal-based feed used in the diet for antelope sitatunga ( ) on feed intake, eating behaviour, rumination and nutrient digestibility. Three male sitatunga were fed a basal diet (chopped dehydrated lucerne, ground cereals, soyabean meal, vegetables and fruits) with access to meadow hay. The animals were allocated to 1 of 3 treatments according to 3 × 3 Latin square design and fed diets where 0, 50 or 100% of dry matter (DM) from basal diet was replaced with a pelleted cereal-based feed. DM intake of the basal diet and meadow hay, as well as overall DM intake and organic matter (OM) intake did not differ between treatments ( > 0.05). The eating rate (g DM · min–1) of the basal diet increased ( = 0.03) whereas eating time (min · day–1) and eating frequency (n · day–1) tended to decrease ( ≤ 0.07) linearly as pelleted feed inclusion in the diet increased. On the other hand, a tendency ( = 0.07) to longer time of hay intake (min · day–1) was observed with increasing inclusion of pelleted cereal-based feed in the basal diet. Rumination frequency (n · day–1), time (min · day–1) and rate (min · g–1 of DM intake) did not differ between treatments ( > 0.05). Apparent total tract digestibility of OM decreased linearly ( = 0.05) with increasing pellet inclusion in the diet. Pelleted cereal-based supplement used in the diet affects feeding behaviour and thus may affect health and welfare of sitatunga in zoological gardens.

Diverse expression of N-acetylglucosaminyltransferase V and complex-type Beta1,6-branched N-glycans in uveal and cutaneous melanoma cells

58%

Pochec E. , Rydlewska M. , Przybylo M. , Litynska A.

nr 2

Differences of alpha3beta1 integrin glycans from different human bladder cell lines

58%

Litynska A. , Przybylo M. , Ksiazek D. , Laidler P.

nr 2

Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of alpha3beta1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of alpha3 and beta1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines alpha3beta1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in beta1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of beta1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in beta1 subunit from Hu456 transitional cell cancer line. On the other hand alpha3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with alpha3 not beta1 subunit.

Characterisation of integrin content in human normal aortic valves and in valves affected by calcific aortic stenosis

51%

Przybylo M. , Stepien E. , Pfitzner R. , Litynska A. , Sadowski J.

tom 07

nr 3