Wyniki wyszukiwania - Biblioteka Nauki

1

Biodegradowalne polimery otrzymane na bazie glikolidu, laktydu, epsylon-kaprolaktonu i TMC w długo-terminowym, kontrolowanym transporcie cyklosporyny A i sirolimusu

100%

Jelonek K. , Kasperczyk J. , Dobrzyński P.

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tom R. 9, nr 58-60

129-132

2

Biodegradowalne polimery z pamięcią kształtu – badania na ludzkich chondrocytach w układzie in vitro

75%

Kłosek K. , Komacki J. , Jelonek K. , Orchel A. , Dobrzyński P. , Kasperczyk J. , Dzierżewicz Z.

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2010

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tom Vol. 13, no. 96-98

121--125

PL

W niniejszej pracy analizowano wpływ substancji wymywalnych, otrzymanych w drodze ekstrakcji z bioresorbowalnych terpolimerów zsyntetyzowanych przy użyciu Zr(Acac)4 i przetwarzanych metodą prasowania, na wzrost chondrocytów ludzkich w warunkach in vitro. Matryce wykonane z poli(L-laktydo- ko-glikolido-ko-węglanu trimetylenu) 75:14:11 oraz poli(L-laktydo-ko-glikolido-ko-ε-kaprolaktonu) 55:29:16 charakteryzowano za pomocą spektroskopii NMR, GPC oraz DSC. Ekstrakcję przeprowadzono w medium hodowlanym z matryc polimerowych przed degradacją oraz po 30, 60 i 90 dniach degradacji. Nie stwierdzono zahamowania wzrostu chondrocytów inkubowanych w obecności ekstraktów uzyskanych z matryc P(L-LA-GA-TMC) 75:14:11 w porównaniu z kontrolą (medium hodowlane) oraz UHMWP, niezależnie od zastosowanego rozcieńczenia i stopnia zdegradowania matryc. Zaobserwowano natomiast zahamowanie wzrostu chondrocytów inkubowanych w obecności rozcieńczonych pożywką ekstraktów (1:4), otrzymanych z matryc P(L-LA-GA-CL) 55:29:16, poddanych 60- i 90-dniowej degradacji.

EN

The influence of substances extracted from bioresorbable terpolymers, synthesized with Zr(Acac)4 as initiator and processed by compression, on human chondrocytes growth in vitro, was analyzed in this study. The matrices obtained from poly (L-lactideco- glycolide-co- trimethylene-carbonate) 75:14:11 and poly (L-lactide-co-glycolide-co-ε-caprolactone) 55:29:16 were characterized by means of NMR spectroscopy, GPC and DSC. Extracts were prepared of polymeric matrices before and after 30, 60 and 90 days of degradation in chondrocytes growth medium. The obtained results did not show growth inhibition of chondrocytes incubated with extracts of P(LA-GATMC) 75:14:11, compared to growth medium (control) and UHMWP (negative control), regardless the used dilution and degree of matrices’ degradation. Growth inhibition was observed in the case of chondrocytes incubated with extracts of P(LA-GA-CL) 55:29:16 matrices after 60 and 90 days of degradation diluted with medium to 1:4.

3

Biocompatibility of L-lactide-co-glycolide-co-trimethylene-carbonate shape memory terpolymer with human chondrocytes

75%

Kłosek R. , Komacki J. , Orchel A. , Jelonek K. , Dobrzyński P. , Kasperczyk J. , Dzierżewicz Z.

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tom Vol. 15, no. 113

23--25

EN

Biomedical application of biodegradable shape memory terpolymers obtained from L-lactide, glycolide, trimethylene carbonate (TMC) have been intensively investigated in recent years. All applicable biomedical materials must be biocompatible, which means that they cannot cause toxic, cytotoxic, allergic, or carcinogenic reactions. The first study evaluating the biocompatibility of the material is determination of its cytotoxicity. The purpose of this study was to assess the biocompatibility of poly(L-lactide-co-glycolide-co-trimethylene-carbonate) 75:13:12, synthesized using Zr(Acac)4 as an initiator of polymerization. The terpolymer was degraded for 30, 60 and 90 days at 37§C in water. The effect of degradation products on the growth of human articular chondrocytes was determined using the sulforhodamine B assay. The examined terpolymer was characterized by means of NMR spectroscopy, GPC and DSC. The results showed that the studied terpolymer was biocompatible with tested cells.

4

Wpływ budowy dwuwarstwowych biodegradowalnych systemów polimerowych na proces uwalniania cyklosporyny A

75%

Jelonek K. , Kasperczyk J. , Dobrzyński P. , Jarząbek B. , Klenczar B. , Sobota M.

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2009

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tom Vol. 12, no. 89-91

182--185

5

Zmiany struktury i wybranych właściwości terpolimerów z pamięcią kształtu wywołanych w procesie otrzymywania implantów metodą wtrysku oraz ich sterylizacji

63%

Jaros A. , Student Z. , Jelonek K. , Sobota M. , Gębarowska K. , Kasperczyk J. , Dobrzyński P. , Smola A.

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2010

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tom Vol. 13, no. 96-98

34--39

PL

W pracy przedstawiono wyniki badań wpływu formowania implantów polimerowych metodą wtrysku oraz ich sterylizacji wiązką elektronów na zmiany struktury i właściwości termicznych trzech różnych terpolimerów LL-laktydu, glikolidu i węglanu trimetylenu z pamięcią kształtu. Polimery charakteryzowano wykorzystując analizę DSC (właściwości termiczne), GPC (masy cząsteczkowe) i 1H NMR (skład i mikrostruktura). Badano materiał wyjściowy po syntezie, uformowane metodą wtrysku matryce polimerowe, sterylne matryce polimerowe i materiał sterylny nie przetworzony. Oceniono przydatność wybranych metod przetwarzania do zastosowania w produkcji biozgodnych implantów polimerowych. Zauważono wpływ stosowanych metod przetwórstwa na badane własności polimerów.

EN

In the present work the result of obtaining implants by injection moulding method and their high energy electron beam sterilization was presented. Changes of microstructure and thermal properties were observed. Three different terpolymers were characterized by using DSC, GPC and 1H NMR method. Raw material and polymer matrices before and after sterilization were examined. Application of this processing method for production of biocompatible and biodegradable shape memory polymer matrices production was estimated. An impact of applied method on tested polymer property was noticed.

6

Wzrost i różnicowanie ludzkich chondrocytów hodowanych na biodegradowalnych trójwymiarowych nośnikach polimerowych

63%

Orchel J. , Orchel A. , Kasperczyk J. , Pamuła E. , Paduszyński P. , Dobrzyński P. , Pałasz A. , Bielecki I. , Jelonek K. , Dzierżewicz Z.

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2008

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tom Vol. 11, no. 81-84

8--11

7

Właściwości powierzchniowe matryc otrzymanych z poli(L-laktydo-ko-glikolidu) z rysperydonem i ich zmiany po dwóch tygodniach degradacji

63%

Turek A. , Jelonek K. , Wójcik A. , Dzierżewicz Z. , Kasperczyk J. , Dobrzyński P. , Marcinkowski A. , Trzebicka B.

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2010

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tom Vol. 13, no. 96-98

117--120

PL

W celu optymalizacji leczenia schizofrenii proponowanych jest wiele rozwiązań. Do jednego z nich należy zastosowanie biodegradowalnych, implantowanych systemów uwalniających leki. W niniejszej pracy badano za pomocą mikroskopii sił atomowych zmiany powierzchni matryc otrzymanych z poli(L-laktydo-koglikolidu) (PLAGA) i rysperydonu przed i po dwóch tygodniach degradacji. Analizowano także wpływ degradacji na zmiany ubytku masy matryc polimerowych. Wyniki wskazują na duże możliwości matryc PLAGA do inkorporowania i pułapkowania rysperydonu. Po dwóch tygodniach stwierdzono stabilność matryc (nie uległy one gwałtownej i niekontrolowanej degradacji). Matryce otrzymane z PLAGA posiadają właściwości powierzchniowe odpowiednie dla zastosowania jako biodegradowalne systemy o kontrolowanym uwalnianiu rysperydonu.

EN

Various solutions are proposed to optimize the therapy of schizophrenia. One of them is the application of biodegradable implantable drug delivery systems. In this work, surface changes of matrices composed of poly(L-lactide-co-glycolide) (PLAGA) and risperidone before and after two weeks of degradation were determined by atomic force microscopy. The influence of degradation on weight loss of matrices was also observed. The results suggest that PLAGA matrices present great potential for incorporation and trapping of risperidone. After two weeks of the studies, the matrices were stable and were not subjected to rapid and uncontrolled degradation. PLAGA matrices have surface properties useful for designing of biodegradable system of controlled risperidone release.

8

Adhezja i wzrost ludzkich chondrocytów na powierzchni bioresorbowalnych materiałów polimerowych

63%

Orchel A. , Dylla A. , Jelonek K. , Kasperczyk J. , Dobrzyński P. , Owczarek A. , Bielecki I. , Dzierżewicz Z.

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tom R. 9, nr 58-60

223-226

9

Thermal properties and morphology changes in degradation process of poly(L-lactide-co-glycolide) matrices with risperidone

63%

Turek A. , Kasperczyk J. , Jelonek K. , Borecka A. , Janeczek H. , Libera M. , Gruchlik A. , Dobrzyński P.

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tom Vol. 17, no. 1

11--20

EN

Determining thermal properties and morphology seems to be useful in the analysis of release and degradation processes form polymeric materials. Risperidone is available in the formulation of a long-acting injection based on poly(D,L-lactide-co-glycolide). Currently, alternative solutions are also offered, i.e., nano- and microparticles or implants, including copolymers of lactide and glycolide. The effect of risperidone content on the properties of poly(L-lactide-co-glycolide) matrices was determined. The study also involved an assessment of the changes during degradation. Risperidone free matrices and the matrices with risperidone were obtained by solvent casting. Thermal characteristics were tested by means of differential scanning calorimetry, and the morphology was evaluated using a scanning electron microscope. Risperidone did not change significantly semi-crystalline structure of poly(L-lactide-co-glycolide) matrices. The decrease in crystallization temperature and glass transition temperature during degradation was observed. Many pores and their deformation, the widening of pore area, cracks and slits because of degradation were observed. The analysis of thermal properties and morphology allowed us to explain degradation process. Matrices exhibited stable process of degradation, which may be advantageous for development of prolonged risperidone release systems.

10

Effect of vascular scaffold composition on release of sirolimus

63%

Jelonek K. , Dobrzyński P. , Kasperczyk J. , Jaworska J. , Pastusiak M. , Sobota M. , Włodarczyk J. , Karpeta-Jarząbek P.

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tom Vol. 21, no. 148

30

11

Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma

63%

Jelonek K. , Wojakowska A. , Marczak L. , Muer A. , Tinhofer-Keilholz I. , Lysek-Gladysinska M. , Widlak P. , Pietrowska M.

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nr 2

12

Influence of surface and structural properties on the initial release of risperidone from polymeric drug carriers

51%

Turek A. , Kasperczyk J. , Jelonek K. , Dobrzyński P. , Walichiewicz J. , Krzemińska K. , Smola A. , Musiał-Kulik M. , Marcinkowski A. , Libera M. , Gębarowska K.

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tom Vol. 15, no. 116-117 spec. iss.

144--146

EN

In this work, implantable drug formulation with risperidone on the basis of poly(L-lactide-co-glycolide) (L-PLGA) and poly(D,L-lactide-co-glycolide) (D,L-PLGA) as drug carries was developed. The influence of surface and structural properties on the initial release of risperidone during the first twenty four hours was determined. In this aim, high-performance liquid chromatography, nuclear magnetic resonance spectroscopy, scanning electron microscope and atomic force microscope were used. Significant differences between L-PLGA and D,L-PLGA matrices in all analyzed data were noted. The burst effect was not revealed for any of the studied polymers, however the released drug was almost five times larger for D,L-PLGA matrices. The L-PLGA copolymer revealed a significantly longer average length of the lactidyl and glycolidyl blocks than D,L-PLGA. Moreover, various characters of surface for analyzed matrices were shown, i.e. in case of L-PLGA the surface was porous and in case of D,L-PLGA it was nonporous. Undoubtedly, there were dependences between risperidone's initial release and the topography and the structure of polymeric matrices. We suppose that the larger drug release for L-PLGA was more associated with surface properties and thus structure of matrices. The obtained results showed the great potential of both polymers and possibility to choose the optimal polymer.

13

Identification of serum proteome components associated with progression of non - small cell lung cancer

51%

Pietrowska M. , Jelonek K. , Michalak M. , Ros M. , Rodziewicz P. , Chmielewska K. , Polanski K. , Polanska J. , Godowicz-Klosok A. , Giglok M. , Suwinski R. , Tarnowski R. , Dziadziuszko R. , Rzyman W. , Widlak P.

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nr 2

EN

The aim of the present study was to perform comparative analysis of serum from patients with different stages of non-small cell lung cancer (NSCLC) using the three complementary proteomic approaches to identify proteome components associated with the progression of cancer. Serum samples were collected before any treatment from 200 patients with NSCLC, including 103 early stage, 64 locally advanced and 33 metastatic cancer samples, and from 200 donors without malignancy. The low-molecular-weight fraction of serum proteome was MALDI-profiled in all samples. Serum proteins were characterized using 2D-PAGE and LC-MS/MS approaches in a representative group of 30 donors. Several significant differences were detected between serum samples collected from patients with early stage cancer and patients with locally advanced cancer, as well as between patients with metastatic cancer and patients with local disease. Of note, serum components discriminating samples from early stage cancer and healthy persons were also detected. In general, about 70 differentiating serum proteins were identified, including inflammatory and acute phase proteins already reported to be associated with the progression of lung cancer (serum amyloid A or haptoglobin). Several differentiating proteins, including apolipoprotein H or apolipoprotein A1, were not previously associated with NSCLC. No significant differences in patterns of serum proteome components were detected between patients with adenocarcinoma and squamous cell carcinoma. In conclusion, we identified the biomarker candidates with potential importance for molecular proteomic staging of NSCLC. Additionally, several serum proteome components revealed their potential applicability in early detection of the lung cancer.