Wyniki wyszukiwania - Biblioteka Nauki

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The relationship between alkaline phosphatase and bone alkaline phosphatase activity and the growth hormone/insulin-like growth factor-1 axis and vitamin D status in children with growth hormone deficiency

100%

Witkowska-Sędek E. , Stelmaszczyk-Emmel A. , Majcher A. , Demkow U. , Pyrżak B.

Acta Biochimica Polonica

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2018

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tom 65

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nr 2

269-275

EN

The relationships between bone turnover, the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis and vitamin D are complex, but still not fully explained. The GH/IGF-1 axis and vitamin D can mutually modulate each other's metabolism and influence the activation of cell proliferation, maturation, and mineralization as well as bone resorption. The aim of this study was to evaluate the reciprocal associations between bone formation markers [alkaline phosphatase (ALP), bone alkaline phosphatase (BALP)], the GH/IGF-1 axis and 25-hydroxyvitamin D [25(OH)D] in children with growth hormone deficiency at baseline and during recombinant human growth hormone (rhGH) therapy. ALP, BALP, 25(OH)D and IGF-1 levels were evaluated in 53 patients included in this prospective three-year study. ALP, BALP and IGF-1 increased during rhGH therapy. Baseline ALP activity correlated positively with baseline height velocity (HV). ALP and BALP activity at 12 months correlated positively with HV in the first year of therapy. We found positive correlations between ALP and IGF-1 at baseline and during the first year of therapy, between BALP activity at 12 months and rhGH dose in the first year of therapy, and between doses of cholecalciferol in the first year of rhGH therapy and early changes in BALP activity during rhGH therapy. Our results indicate that vitamin D supplementation enhances the effect of rhGH on bone formation process, which could improve the effects of rhGH therapy. ALP and BALP activity are useful in the early prediction of the effects of rhGH therapy, but their utility as long-term predictors seemed insufficient.

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Rola Chlamydia pneumoniae w patogenezie przerostu i zapalenia tkanki migdałka gardłowego u dzieci

86%

Bielicka A. , Zielnik-Jurkiewicz B. , Podsiadły E. , Prochorec-Sobieszek M. , Rogulska J. , Demkow U.

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tom 70

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nr 5

7-12

PL

STRESZCZENIE: Wstęp. Powinowactwo do komórek nabłonka i limfocytów, zdolność do hamowania apoptozy zakażonych komórek gospodarza oraz występowanie w formie przetrwałej niewrażliwej na antybiotyki – to cechy Chlamydia pneumoniae, które mogą być związane z przewlekłym stanem zapalnym migdałka gardłowego i jego przerostem. Celem pracy była odpowiedź na pytania: 1) Czy w migdałku gardłowym u dzieci zakwalifikowanych do adenoidektomii występuje C. pneumoniae? 2) Czy występuje zależność między obecnością C. pneumoniae w migdałku gardłowym a wielkością migdałka gardłowego? 3) W których komórkach migdałka gardłowego najczęściej występuje C. pneumoniae? Materiał i metody. Grupę badaną stanowiło 200 dzieci w wieku od 2 do 16 lat (średni wiek – 6,4) wybranych spośród pacjentów zakwalifikowanych do adenoidektomii. U wszystkich podczas kwalifikacji do zabiegu wykonywano badanie fiberoskopowe nosogardła. Tkankę usuniętego migdałka gardłowego badano metodą real-time PCR w kierunku C. pneumoniae. Migdałki pobrane od dzieci z dodatnim wynikiem badania metodą PCR oraz od 10 losowo wybranych dzieci z ujemnym wynikiem tego badania, oceniano stosując badanie immunohistochemiczne (IHC). Wyniki. DNA C. pneumoniae w usuniętym migdałku gardłowym stwierdzono u 5,5% dzieci. Dodatnie wyniki uzyskiwano najczęściej w grupie wiekowej od 10. do 16. roku życia (24,1%, 7/29). Wykazano zależność między występowaniem C. pneumoniae w migdałku gardłowym a wielkością migdałka gardłowego. U wszystkich dzieci z dodatnim wynikiem badania metodą PCR potwierdzono obecność C. pneumoniae w migdałku gardłowym przy zastosowaniu IHC. Najczęściej wykrywano C. pneumoniae w limfocytach oraz w komórkach nabłonka. Wnioski. Obecność C. pneumoniae w limfocytach i komórkach nabłonka migdałka gardłowego głównie u dzieci starszych z przerostem migdałka gardłowego potwierdza udział tej bakterii w procesach patologicznych tkanki migdałka gardłoweg

3

Role of Chlamydia pneumoniae in the pathogenesis of hypertrophy and adenoid tissue inflammation in children

86%

Bielicka A. , Zielnik-Jurkiewicz B. , Podsiadły E. , Prochorec-Sobieszek M. , Rogulska J. , Demkow U.

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tom 70

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nr 5

7-12

EN

Objective. A tropism to epithelial cells and lymphocytes, an inhibition of apoptosis in host cells, an ability to occurrence in persistent form resistant to antibiotic treatment are the features of Chlamydia pneumoniae, which can have connection with chronic inflammation of an adenoid tissue and adenoid hypertrophy. This study aimed to (1) detect the C. pneumoniae in an adenoid in children undergoing adenoidectomy, (2) estimate a connection between C. pneumoniae occurrence and the size of adenoid, (3) demonstration in which of adenoid cells C. pneumoniae occurs most often. Material and methods. The examined group consisted of 200 children aged from 2 to 16 years (mean age 6,4) undergoing adenoidectomy. In all children during qualification for adenoidectomy a fiberoscopic examination of the nasopharynx was performed. A part of removed adenoid tissue was analysed by real-time PCR for C. pneumoniae. Adenoids from children with positive PCR examination and from 10 children with negative PCR examination were examined using immunohistochemistry (IHC). Results. C. pneumoniae in the adenoid was present in 5,5% children. Positive results were obtained most frequently (24,14%, 7/29) in the eldest group (10-16 years). A statistical analysis demonstrated the correlation between C. pneumoniae occurrence in an adenoid tissue and the size of adenoid. In immunohistochemistry C. pneumoniae was found the most frequently in lymphocytes and in epithelial cells. Conclusions. A presence of C. pneumoniae in lymphocytes and epithelial cells of the adenoid first of all in older children with adenoid hypertrophy confirms the participation of this bacteria in adenoid pathology.

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Mitochondrial DNA in pediatric leukemia patients

86%

Kodroń A. , Ghanim M. , Krawczyk K. , Stelmaszczyk-Emmel A. , Tońska K. , Demkow U. , Bartnik E.

Acta Biochimica Polonica

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2017

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tom 64

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nr 1

183-187

EN

Numerous studies of mitochondrial DNA (mtDNA) in cancer have shown differences between mtDNA sequences in tumor and normal tissue and at various stages of cancer treatment in the same patient. However, there is little data on acute lymphoblastic leukemia (ALL), the most common type of leukemia in children. In this study we compared mitochondrial sequence variation in the D-loop region and in 5 genes of mtDNA in bone marrow samples of 6 pediatric patients with ALL at various stages of therapy. We found several common polymorphisms and one variant at position 3688 whose level varied during leukemia treatment. Our results suggest that mitochondrial DNA mutations, whose levels change during patient treatment, could be potential biomarkers for monitoring treatment efficacy and disease progression.