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EN
Bound-constrained Support Vector Machine(SVM) is one of the stateof- art model for binary classification. The decomposition method is currently one of the major methods for training SVMs, especially when the nonlinear kernel is used. In this paper, we proposed two new decomposition algorithms for training bound-constrained SVMs. Projected gradient algorithm and interior point method are combined together to solve the quadratic subproblem effciently. The main difference between the two algorithms is the way of choosing working set. The first one only uses first order derivative information of the model for simplicity. The second one incorporate part of second order information into the process of working set selection, besides the gradient. Both algorithms are proved to be global convergent in theory. New algorithms is compared with the famous package BSVM. Numerical experiments on several public data sets validate the effciency of the proposed methods.
EN
β-cyclodextrin (β-CD) and its derivatives have been widely used to prepare inclusion complexes. However, systematic research on their thermal stabilities, pyrolysis characteristics and kinetics has rarely been reported. In this paper, thermogravimetric analysis was employed to investigate β-cyclodextrin and its two derivatives, 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) and monochlorotriazinyl-β-cyclodextrin (MCT-β-CD). The pyrolysis characteristics and kinetic parameters were obtained. The results show that three stages can be distinguished during the heating process of the above three samples. The temperature of initial decomposition of HP-β-CD (309.5°C is higher than that of β-CD (297.8°C), while the temperature of initial decomposition of MCT-β-CD (231.4°C) is lower than that of β-CD. For the three cyclodextrins, the thermal stability in descending order is HP-β-CD, β-CD and MCT-HP-β-CD. The activation energy values are 350.6, 303.3 and 113.9 KJ/mol, and the pre-exponential factor values are 1.11×1031, 1.37×1026 and 1.39×1010 for β-CD, HP-β-CD and MCT-β-CD respectively.
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nr 1
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Melanophilin (MLPH) gene has been characterized as one of the candidate genes for coat colour dilution in human, mice and dog, but little is known about it in goat. Part of the 5’UTR (650bp,JQ743911) was sequenced leading to identify four polymorphic loci (g.G426A, g.A570G, g.G592T and g.612-614insCTC). The genetic diversity was analysed in 120 individuals of four breeds of goat. The possible transcription factors in the four loci were mined by online tools. The results show that the four loci were in Hardy-Weinberg equilibrium (P>0.05) and there was a significant difference in gene sequence between Jining Grey (JN) and Leizhou Black (LZ) breeds (P < 0.05). JN has the highest allele frequency of A, A, G and insertion CTC at the four loci; and LZ has the highest allele frequency of G, G, T and deletion CTC. JN has the lowest effective allele number and the lowest value of Shannon I at the four polymorphic loci. LZ has the highest effective allele number and the highest Shannon I at the four polymorphic loci. Moreover, JN has the highest Fst and Gst (0.08 and 0.12, respectively) and the lowest Nm (2.80) compared to LZ. In silico analysis showed that the possible transcription factors having a potential binding site in the sequence consisting of A, A and G and insertion CTC in the four polymorphic loci of 5’UTR of MLPH gene were BRN2, CCAT,P, STAT and USF. The sequence consisting of G, G, T and deletion CTC in the 5’UTR region of MLPH formed the possible binding sites of MyoD, c-Myb, v-Myb, AP-1, USF and NKX25. It could be concluded that JN has different gene sequence in the four mutation sites of 5’UTR of MLPH compared to LZ and the mutation caused the variation of possible transcription factors that may play a role in the MLPH gene expression regulation; so JN has dilute, ashen and grey coat colours and LZ is of solid black colour.
EN
On a global basis, cash cropping is usually identified as an important enterprise undertaken by farmers to increase incomes. However, the responses of farmers to these new enterprises vary greatly. Through a case study of a Dai village in Xishuangbanna, China, this paper examines how farmers make decisions about adopting new cash crops by focusing on the farm economy and land conditions. The results show that farmers did not adopt watermelons due to poor irrigation and accessibility conditions, and then they did not adopt bananas due to a transient collapse of banana market, induced by a rumor suggesting that eating bananas causes cancer. Consequently, although these non-adopters benefited from commercial exchanges with external businessmen in terms of ecological experiments and management diversification, and leasing lowlands to external businessmen is a livelihood choice that is based on the outcome of the farmer’s trade-off between profitability and risk, they missed opportunities to substantially increase incomes through cash cropping, as evidenced by the success of the farmers who adopted the cash crops. These findings suggest that the government ought to design tailored extension programs for villages, implement efficient refutation strategies to prevent rumor-induced market collapse and promote extension services as early as possible in the initial stages of transition to cash cropping.
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Patrząc z perspektywy globalnej za uprawy komercyjne uznaje się istotne działania podejmowane przez rolników w celu zwiększenia swoich dochodów. Jednakże zainteresowanie rolników nowymi rozwiązaniami jest bardzo zróżnicowane. Niniejszy artykuł na przykładzie doliny Dai w należącym do Chin rejonie Xishuangbanna pokazuje jakie czynniki finansowe i środowiskowe wpływają na podjęcie przez rolników decyzji odnoszących się do nowych upraw komercyjnych. Otrzymane wyniki pokazują, że za odrzuceniem arbuzów stały ograniczone możliwości nawadniania i ograniczony dostęp, a za odrzuceniem bananów chwilowe załamanie rynku zbytu tych owoców, związane z szerzącą się pogłoską, jakoby konsumpcja bananów mogło być przyczyną nowotworów.Konsekwentnie, chociaż rolnicy odrzucający nowe uprawy korzystali z wymiany handlowej z zewnętrznymi przedsiębiorcami pod kątem eksperymentów ekologicznych i dywersyfikacji zarządzania, a ponadto biorąc pod uwagę, że przeznaczanie pól dla zewnętrznych przedsiębiorców jest decyzją opartą na ocenie możliwych zysków i strat, ci rolnicy stracili szansę na znaczące zwiększenie przychodów z upraw komercyjnych. Pokazuje to przykład rolników, którzy jednak zdecydowali się na nowe uprawy. Uzyskane rezultatu wskazują, że rząd powinien przygotować dostosowane do potrzeb rolników programy i strategie odnoszące się do tych zagadnień.
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Although the relationship between basal (BT), acquired (AT) and maintenance of acquired thermotolerace (MT) has been illustrated in a heat resistant tomato genotype and sensitive tomato genotypes, whether more resistant and sensitive tomato genotypes are satisfied with the rule and the relationships of antioxidant enzymes (AE) activity in three kinds of thermotolerance are not known. Here, we have observed the intension of three kinds of tomato thermotolerance is associated with AE activities. The priming and enhancement of thermotolerance was temperature dependent with stronger thermotolerance priming by moderately high temperatures than warmer temperatures. Interestingly, AE activity also showed significantly higher in seedlings under moderately high temperature than ones at warmer temperatures. While after the first optimized priming of different high temperatures combined with secondary priming for a reasonable period, AE activity and its thermotolerance further enhanced. Surprisingly, these optimized acclimation treatments showed no difference in AE activity and MT intension, suggesting that secondary priming could supply gaps produced by the first priming of warmer high temperatures, through enhancing AE activity. Additionally, the basal heat resistant genotypes showed stronger AE activity and thermotolerance (AT and MT) than sensitive genotypes. Results from this study will provide insights into understanding mechanism behind regulating tomato thermotolerance and facilitate the development of heat tolerant cultivars.
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tom 26
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nr 5
EN
Phosphorus as a major nutrient element in water ecosystems is a key factor affecting algae growth in rivers. This paper adopts the sequential extraction method for phosphorus fractionation to study the distribution of phosphorus forms in riparian soils and peripheral river sediments, then to analyze the relationship between different phosphorus forms and their significance within a watershed environment. The results show that: 1) the contents of NH₄Cl-P, BD-P, and HCl-P in riparian soils are more widespread than those in river sediments, 2) the distribution patterns of phosphorus fractions in different catchments of Jianxi Basin are varied for soil and sediment, and 3) the ratio of BAP/TP is the highest in Chongyangxi catchment and the lowest in Songxi catchment. Phosphorus in riparian soils is mainly affected by agricultural activities, significantly for NaOH-P and HCl-P. And phosphorus forms in sediments are obviously affected by domestic sewage. The phosphorus contents and fractions in riparian soils change more significantly than those in river sediments.
EN
In this paper, ionic gelation method was adopted to produce nanocapsules (CNs) encapsulated tuberose fragrance with chitosan (CS) and sodium tripolyphosphate (TPP) as wall materials. The effects of CS/TPP mass ratio, pH value of CS solution, molecular mass of CS and tuberose fragrance (TF) concentration on particle size and particle size distribution (PDI) of chitosan nanocapsules encapsulated tuberose fragrance (CNTs) were investigated systematically. CNTs were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analyzer (TGA) and differential scanning calorimetry (DSC). The results showed that CNTs were successfully prepared. The optimum preparation conditions were as follows: CS/TPP mass ratio 5:1, pH of CS solution 4.0, and molecular mass of CS 150 kda. CNTs emulsions were also systematically investigated by steady-state shear and oscillatory shear measurements respectively. The rheological behaviors of CNTs were obtained.
EN
l-menthol has been widely used in flavour, food and pharmaceuticals. Because of its high volatility and whisker growth, l-menthol-hydroxypropyl-β-cyclodextrin inclusion complex was produced to improve shelf-life, provide protection, and enhance the stability of l-menthol. The inclusion complex was characterized by Fourier transform infrared spectroscopy, X-ray diffraction. The results show that l-menthol was successfully encapsulated in hydroxypropyl-β-cyclodextrin. l-menthol loading capacity is about 8.44%. Geometries and binding energies of l-menthol-hydroxypropyl-β-cyclodextrin inclusion complexes were investigated using molecular mechanics calculations. The shape and orientation of the most stable complex, and the minimum binding energy were determined. L-menthol release from complex was determined by thermogravimetric analysis. Two l-menthol release rate peaks were observed at 69.3 and 279.1°C. The l-menthol release reaction order, release activation energy and the preexponential factor were obtained.
EN
To understand physiological acclimation of psammophyte to repeated soil drought and rewatering, two psammophytes (Setaria viridis and Digitaria ciliaris) were subjected to three cycles of soil drought and rewatering. The response process of leaf relative water content (RWC), membrane permeability, lipid peroxidation, gas exchange characteristics, antioxidant enzymes, soluble protein, and free proline was examined. Leaf RWC, the net photosynthesis rate, stomatal conductance, and water use efficiency decreased, while membrane permeability, lipid peroxidation, intercellular CO₂ concentration, soluble protein, and free proline increased during three soil drought periods for both psammophytes. These physiological characteristics were recovered to the control levels following rewatering for 4 days. However, activities of SOD, CAT, and POD were induced continuously under soil drought conditions, and remained higher than those in the control throughout the whole experiment period, which agrees with our hypothesis that drought hardening activates defensive systems of both psammophytes continuously. Decreasing level of leaf RWC and increasing levels of leaf membrane permeability and lipid peroxidation were suppressed with increasing the number of drought cycles, suggesting that drought hardening alleviates damages of both psammophytes and improves their drought tolerance and acclimation to soil drought conditions in the future. Additionally, the photosynthesis decreased more slowly in the subsequent drought cycles than in the first cycle, allowing both psammophytes to maximize assimilation in response to repeated soil drought conditions. Thus, both psammophytes acclimatize themselves to repeated soil drought.
EN
As a member of tyrosinase-related family, tyrosinase-related protein-1 (TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about its role in goat. In this study, almost complete goat TYRP1 gene (17554 bp, HMO70243) encompassing complete CDS was identified, the coding region amounting to 1614 bp, distributed in 7 exons (2-8), and intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeat (SSR) motifs were detected in goat TYRP1 gene, in which 4 SSR motifs in intron 5 are combined in a tandem. Correlation analysis showed the allele A to be advantageous compared to C at site g.1263A>C and allele C compared to T at g.1428C>T, implyimg that haplotype AC is in favour of eumelanin biosynthesis, and haplotype CC is in favour of pheomelanin formation.Marked deficiency of heterozygotes occurred in Nanjiang Yellow Goat Black strain and Nanjiang Yellow Goat Fast Grow strain, indicating the high inbreeding in both strains.
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Sonneratia alba (S. alba) is a mangrove species grown in brackish water of tropical and subtropical regions. Due to its unique environment, it has evolved various mechanisms for modulating salt and metal levels. In order to find the genes connected with bioaccumulation of metals, the root transcriptome annotation of Sonneratia alba was analyzed and a new metallothionein (MT) gene was cloned. Sequence analysis found that the new MT gene belongs to type 3 MT, which is mostly expressed in roots. A simple and efficient method was used to express the type 3 MT of S. alba (SaMT3) by transforming the recombinant expression vector pET15b-SaMT3 into Escherichia coli (E. coli) Rosetta-gami and induction with the optimal conditions of 500 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 24ºC for 12 h. OD₆₀₀ of E. coli cells expressing His fused SaMT3 protein after treated with 500 μM Cu²⁺ or 500 μM Pb²⁺ for 12 h can reach 1.01 or 0.98, while OD₆₀₀ of control cells expressing His-tag can reach only 0.81 or 0.75. Both control cells and the cells expressing SaMT3 accumulated metals. Cells expressing SaMT3, however, accumulated more Pb²⁺ and Cu²⁺ (more than two times) than control cells. In vivo, real-time PCR showed that the SaMT3 transcript was induced significantly when stimulated with 250 μM, 500 μM, or 1,000 μM Cu²⁺ or Pb²⁺ for 24 h and 48 h. Taken together, the expression of SaMT3 can increase Cu²⁺ and Pb²⁺ resistance and binding capacity of E. coli.
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