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3 Journal of Applied Genetics

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Evaluation of the effect of centromeric heterochromatin polymorphism on pig fertility

100%

Kozubska-Sobocinska A. , Danielak-Czech B. , Slota E. , Rejduch B.

Journal of Applied Genetics

1996

tom 37

nr 3

293-298

C-banding identified centromeric heterochromatin polymorphism, most often located in a pair no 16, was a basis for selecting animals for the experiment.The aim of the experiment was to assess the impact of centromeric heterochromatin polymorphism on pig fertility, expressed by litter size.The research included the first two litters obtained from 30 pairs of parent with different genotypes as regards the parameter under study.A statistical analysis of the number of offspring from different mating types showed no significant differences between the experimental groups of animals.The results obtaned did not confirm suggested correlation between polymorphism of the centromeric heterochromatin regons and pig fertility, estimated by mean size.Thus the polymorphism cannot be regarded as a selection criterion.

The application of human band specific probe (HSA11p15) to identify a corresponding region in the porcine genome

100%

Rejduch B. , Slota E. , Kozubska-Sobocinska A.

nr 3

341-342

The aim of this study was to analyse homology in the telomeric region of chromosome 11 in humans and a corresponding fragment in the porcine genome using the FISH technique. The human band specific probe (HSA 11p15) was used for hybridization with pig chromosomes. The obtained results showed strong signals on human chromosome 11p15, as well as pig chromosome 2p17. Some aspects of the similarity between the human and pig chromosome segments have been discussed.

Detection of the XXY trisomy in a bull by using sex chromosome painting probes

88%

Slota E. , Kozubska-Sobocinska A. , Koscielny M. , Danielak-Czech B. , Rejduch B.

tom 44

nr 3

379-382

Two cattle chromosome painting probes, identifying X and Y heterosomes, were applied to verify the diagnosis of XXY trisomy in an 8-month-old bull of the Polish Red breed. The probes were obtained after chromosome microdissection and labelled with biotin-16-dUTP. In all metaphase spreads, three fluorescence signals were observed - two X and one Y - confirming the diagnosis of a pure XXY trisomy.