The content of fatty acids extracted from the membranes of E. coli MC1061 harboring the wild-type dnaKdnaJ alleles and its AdnaJ and AdnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42°C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and octadecanol, the latter being correlated with the presence of octadecanoic acid. The amount of LPS extracted from AdnaKdanJ mutant was significantly lower when compared with wild-type strain and AdnaJ mutant.
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