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2 Progress in Health Sciences

2 2013

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Influence of cervical canal shape on embryo transfer feasibility

100%

Mrugacz G. , Grygoruk C. , Grusza M. , Pietrzycki B. , Ratomski K. , Pietrewicz P.

nr 1

78-82

Purpose: To investigate the impact of cervical canal shape on embryo transfers feasibility. Materials and methods: A retrospective study on the influence of the cervical canal shape on ET complications was conducted among one thousand patients undergoing embryo transfers in the period between 01.2011- 08.2012. The patients, based on ultrasound appearance of the cervical canal, were allocated into one of the following groups: group “I” with straight cervical canal, group “J” with cervical canal bended one time, group “C” with cervical canal bended two times in the same direction and group “Z” with cervical canal bended two times in the opposite direction. Results: Out of the one thousand cases studied, the most prevalent cervical canal shapes were type I (440 cases) and type J (321 cases), 19% (185) of the women had a type C cervix and 5% (54) a type Z cervix. The highest rate of uncomplicated embryo transfers was noted in single bended cervical canals, 85%. Double bended cervical canals were characterized by a high rate of complications during embryo transfer reaching 80%. Conclusions: The results of the present study indicated that the shape of cervical canal has a great influence on embryo transfer feasibility. Special attention should be given to patients with double bended cervical canal, where the rate of complication is highest.

Influence of pressure changes during embryo transfer on blastocyst viability.

100%

Mrugacz G. , Grygoruk C. , Gajda B. , Grad I. , Grusza M. , Sieczynski P.

nr 1

null-89

Purpose: The embryo transfer into the uterus by a transcervical catheter is the final stage of in-vitro fertilization procedure. So far, a little attention has been placed on the impact of embryo transfer procedure on embryo viability. The study was design to measure the morphological changes and apoptosis rate in embryos exposed to embryo transfer in in-vitro conditions. Methods: Morphologically normal rabbit blastocysts were divided randomly into control (48) and experimental (48) groups. The experimental group blastocysts were exposed to embryo transfer in in-vitro conditions. Morphological changes in response to embryo transfer were assessed 5 and 60 minutes after ET. The apoptosis rate was measured one hour after embryo transfer. Results: Morphological changes in response to embryo transfer were more prevalent in the experimental group; 14 shrunken and one collapsed blastocysts in experimental group and only two shrunken blastocysts in the control group. The mean DNA fragmented nucleus index in the experimental group was 37.7 % and was significantly higher than in the control group, 8.1 %. Conclusions: Embryo transfer can trigger both morphological and apoptotic changes in rabbit blastocysts. The local pressure fluctuations during embryo transfer could be a major factor responsible for the above-mentioned changes.