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EN
Eighteen tomato genotypes, with varying degree of response to Alternaria leaf blight disease (ALBD) were used to assess the possible involvement of protease and peroxidase activities in disease response. Preinfectional protease activity varied noticeably in tested genotypes. Highest pre-infectional protease activity was observed in susceptible genotype CLN-2123. Post-infectional protease activity level was generally lower when compared with pre-infectional level in all genotypes with exception of unchanged level in Tibrido. There was no correlation between post-infectional protease activity and percent disease index (%DI). In contrast, pre- and postinfectional leaf peroxidase activities showed a significant (p<0.01) negative correlation with %DI. Genotypes with higher pre-infectional peroxidase activity performed better on exposure to Alternaria alternata infection and accumulate enhanced peroxidase activity. Tibrido accumulated highest peroxidase activity while level was lowest in 1621P, which showed highest ALBD incidence. Moreover, genotypes with better resistance to A. alternata infection maintained higher post-infectional peroxidase activity. In resistant (Tibrido) and all moderately resistant genotypes, leaf peroxidase activity raised after inoculation when compared with the pre-inoculation level. I summary, higher pre- and post-infectional peroxidase activity was found to be associated with Alternaria leaf blight resistance. The peroxidase activity can be used as a biochemical tool in marker-assisted screening of tomato germplasm for Alternaria leaf blight resistance.
EN
New types of dyestuff moieties are being introduced to get the ease in imparting color to various substrates and sorting out the ways to improve the quality of dyed fabric using environmentally friendly techniques. To contribute to this research effort, the present study was carried for dyeing cellulosic fabric using novel bi-functional reactive dye. The synthesized dye contained sulphatovinylsulfone and monochlorotriazine functionality. The important influencing parameters for the exhaust method, e.g., dyeing temperature, concentration of salt, and alkali were optimized and their interaction was studied using central composite design. The results indicated that 61.5ºC was found to be suitable for the maximum exhaustion and fixation of the dye on cotton fabric. The optimum concentration of salt (30.01 g/L) and alkali (22.32 g/L) for dye experimentally resulted in 84.98 (±3)% of exhaustion and 79.00 (±3)% fixation on cellulose fabric. Fastness properties were good-to-excellent for the novel reactive dye applied on cotton fabric. A comparison of the results with the commercial reactive dye showed that attained results were within a practical range suitable for commercial dyeing.
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